Bioartificial liver system

Abstract

The invention belongs to the technical field of biomedicine, and provides a bioartificial liver system. The system comprises a plasma separation / retransformation circulation part and a biological liver circulation part. The two parts are connected with each other through a circulation pipeline. The biological liver circulation part comprises a constant-temperature box with a heat preservation effect, a double-cavity blood storage bag, an oxygenation column, a plasma circulating pump, a dissolved oxygen monitoring and temperature instrument and a flow guide type micro capsule suspended fluidized bed bioreactor, wherein the double-cavity blood storage bag, the oxygenation column, the plasma circulating pump, the dissolved oxygen monitoring and temperature instrument and the flow guide type micro capsule suspended fluidized bed bioreactor are sequentially connected to form a circulating system. By means of a turbine flow guide type inlet, flow distribution is carried out on a part of high-speed fluid of the inlet by the bioreactor, lateral shear force borne by micro capsules in a fluid force field is effectively reduced, damage to the micro capsules is reduced, and contact efficiency of cells cultured in the bioreactor and perfusate is improved. The bioreactor is used for bioartificial liver supportive treatment system in order to obtain more ideal effects on the aspects of treating serious hepatitis, hepatic insufficiency and other diseases.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to an artificial liver medical system. Background technique [0002] As one of the effective treatment methods for patients with severe hepatitis and liver failure, artificial liver support therapy has been recognized all over the world. It includes three categories: non-biological type, biological type and mixed type. At present, biological and hybrid artificial liver support therapy has become a research hotspot of artificial liver. Among them, the bioartificial liver mainly includes the plasma separation / reinfusion circulation part and the biohepatic circulation part. Among them, the biological hepatic circulation part is the main body, which is based on the culture of liver cells and uses the bioreactor as the core carrier to provide functions such as secretion, metabolism, biotransformation and detoxification. The role of the plasma separation part is to separate the blood ...

AI Technical Summary

Problems solved by technology

In response to these shortcomings, Li Lanjuan's laboratory developed a microcapsule suspension fluidized bed bioreactor for artificial liver (patent number: ZL 200820167026.5), and built a bioartificial liver system based on it. Animal experiments showed that the The system has a good curative effect, but it is also found that there are still some areas for improvement and improvement: 1. A high liquid flow rate in the reactor is required to fully suspend and fluidize the microencapsulated hepatocytes, but the microencapsulated cells The fluid shear force that the capsule can withstand is limited; 2. The fluid mechanics test has confirmed that there is a flow dead space in the funnel-shaped fluidized bed bioreactor, and some microencapsulated hepatocytes cannot fully contact the fluidized liquid; The liquid inlet of capsule-suspended fluidized bed bioreactor is small, and increasing the flow rate can lead to too fast local liquid flow at the inlet, and the fluid shear force on local microcapsules is too large, which in turn leads to an increase in the microcapsule breakage rate

4. The dissolved oxygen content must be detected at the inlet and outlet of the reactor, and the previous contact detection method cannot guarantee the biological safety during the treatment process

CN103170021 (flow-guiding microcapsule suspension fluidized bed bioreactor for artificial liver) discloses a flow-guiding microcapsule suspension fluidized bed bioreactor, but the artificial liver system disclosed at that time needs further improvement

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