39 results about "Associated Study" patented technology

The invention discloses a reference gene developed based on a transcriptome sequence of miscanthus sinensis and application thereof. The three reference gene sequences of the invention come from the transcriptome sequence of miscanthus sinensis, and have the advantages of good specificity and high stability compared with the previous reference genes commonly used in other species. At the same time, the expression analysis of SOD and CAT genes under drought stress of miscanthus sinensis proves that the developed three reference genes have better calibration capability than other general reference genes, and provides an effective reference gene calibration tool for the analysis, screening and validation of the gene expression of miscanthus sinensis in the future. In addition, the newly developed reference gene enriches the available number of plant reference genes, as well as is applied to the related research on other andropogoneae and miscanthus plants. The reference gene provided by the invention is suitable for popularization and application in the field of reference genes.

The present invention amplifies the nucleoprotein gene N of SVCV through molecular biology techniques, and constructs recombinant plasmid pMD TM 18‑T‑SVCV‑N, using this plasmid as a template to establish an absolute quantitative SVCV copy number method based on the TaqMan probe method qPCR as a standard curve. At the same time, the protein concentration of SVCV was measured by Bradford method, and the correlation with the copy number of SVCV measured by the qPCR method was established. For the first time, a method for directly measuring protein concentration to determine the copy number of SVCV was established. This method can realize the simple and low-cost determination of the copy number of SVCV, and at the same time has important reference significance for the quantification of other viruses, and has important value in the related research of SVCV and other viruses.

The invention belongs to the field of biotechnology, particularly discloses a primer group and method for specific amplification of Cricetulus and Lasiopodomys Vkorc1 gene coding sequence region. Theprimer group includes 3 pairs of primers for respectively amplifying three exons of Vkorc1 genes, the primer sequence for amplifying the first exon is shown in SEQ ID NO.1-2, the primer sequence for amplifying the second exon is shown in SEQ ID NO.3-4, and the primer sequence for amplifying the third exon is shown in SEQ ID NO.5-6. The invention provides the amplification method based on the primer group through optimizing the amplification condition, the Cricetulus and Lasiopodomys Vkorc1 genes are amplified, and a good foundation is laid for previous related research on the Cricetulus and Lasiopodomys Vkorc1 genes and resistance to drugs.