Construction method of gene overexpression chimeric animal model based on hNPY and hAgRP and application

A gene overexpression, animal model technology, applied in the biological field, can solve problems such as unfavorable large-scale application, long animal model cycle, poor character stability, etc., to achieve the effect of short construction period, convenient large-scale application and low cost

Pending Publication Date: 2020-05-19
四川横竖生物科技股份有限公司
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Problems solved by technology

In the process of completing the present invention, the inventors found that the method takes a long time to construct an animal model, is grea

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  • Construction method of gene overexpression chimeric animal model based on hNPY and hAgRP and application
  • Construction method of gene overexpression chimeric animal model based on hNPY and hAgRP and application
  • Construction method of gene overexpression chimeric animal model based on hNPY and hAgRP and application

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[0048] In order to make it easier for those skilled in the art to further understand the present invention, and to clearly understand the technical solutions described in this application, and to fully and fully disclose the relevant technical content of the present invention, the specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings Description, obviously, the described specific implementation is only an example to give a part of the embodiments of the present invention, to help understand the present invention and its core idea.

[0049] Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative work, and / or without departing from the spirit and essence of the present invention, even if the order of the various steps Changes have been made, and various corresponding changes and deformations have been made according to the pres...

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Abstract

The invention discloses a construction method of a gene overexpression chimeric animal model based on hNPY and hAgRP and application of the construction method. The method comprises the following steps: taking constructed Synapsin as a promoter to drive an hNPY and hAgRP lentiviral expression vector; injecting the vector into the paraventricular nucleus of macaque; and transcribing hNPY and hAgRPinto paraventricular nucleus cell gene to realize overexpression of hNPY and hAgRP genes, and inducing phenotype generation to form the gene overexpression chimeric animal model based on the hNPY andhAgRP. The method can be used for pathogenesis research of related diseases, screening, verification and testing of therapeutic drugs researched by clinical treatment means, pre-clinical related research and the like. The method has the characteristics of short animal model construction period, high efficiency, low cost, high phenotypic explicit rate, good synchronism, high repeatability, low difficulty, convenience in large-scale application and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the construction technology of animal models, in particular to the construction method and application of animal models realized in somatic cells by transgenic technology. Background technique [0002] Animal disease models (animal models) are mainly important tools for research in experimental physiology, experimental pathology and experimental therapeutics (including new drug screening). The development of human diseases is very complicated. Using humans as experimental objects to deeply explore the mechanism of disease occurrence and promote the development of medicine has come slowly. The accumulated clinical experience is not only limited in time and space, but also many experiments are morally unreasonable. and methods are also limited. With the help of indirect research on animal models, factors that are impossible or difficult to exclude under natural conditions can be consciou...

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Application Information

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IPC IPC(8): C12N15/867A01K67/027
CPCG01R33/44A61B5/055
Inventor 王正武黄行许赵旭东樊均明唐珂周涛
Owner 四川横竖生物科技股份有限公司
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