Construction method of gene overexpression chimeric animal model based on hNPY and hAgRP and application

Abstract

The invention discloses a construction method of a gene overexpression chimeric animal model based on hNPY and hAgRP and application of the construction method. The method comprises the following steps: taking constructed Synapsin as a promoter to drive an hNPY and hAgRP lentiviral expression vector; injecting the vector into the paraventricular nucleus of macaque; and transcribing hNPY and hAgRPinto paraventricular nucleus cell gene to realize overexpression of hNPY and hAgRP genes, and inducing phenotype generation to form the gene overexpression chimeric animal model based on the hNPY andhAgRP. The method can be used for pathogenesis research of related diseases, screening, verification and testing of therapeutic drugs researched by clinical treatment means, pre-clinical related research and the like. The method has the characteristics of short animal model construction period, high efficiency, low cost, high phenotypic explicit rate, good synchronism, high repeatability, low difficulty, convenience in large-scale application and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology and relates to the construction technology of animal models, in particular to the construction method and application of animal models realized in somatic cells by transgenic technology. Background technique [0002] Animal disease models (animal models) are mainly important tools for research in experimental physiology, experimental pathology and experimental therapeutics (including new drug screening). The development of human diseases is very complicated. Using humans as experimental objects to deeply explore the mechanism of disease occurrence and promote the development of medicine has come slowly. The accumulated clinical experience is not only limited in time and space, but also many experiments are morally unreasonable. and methods are also limited. With the help of indirect research on animal models, factors that are impossible or difficult to exclude under natural conditions can be consciou...

AI Technical Summary

Problems solved by technology

In the process of completing the present invention, the inventors found that the method takes a long time to construct an animal model, is greatly affected by individual animals and the environment, and has poor stability of properties, which is not conducive to large-scale application

Images