Fluorescent quantitative reference genes of different tissues of siberian wildrye and primers and application thereof
An internal reference gene, fluorescence quantitative technology, applied in the field of plant molecular biology, can solve the problem of less research on gene specific expression
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[0035] 1. Test materials
[0036] The test material was ZHN03 wild sage wheat germplasm collected from Zhuoni County, Gansu Province (see Table 1 for specific information). Place the seeds of the ZHN03 germplasm in a petri dish to germinate for 2-3 weeks, and then transplant the seedlings into a flowerpot (10cm in diameter). The flowerpot is filled with turfgrass soil, vermiculite and sand (volume ratio: 2: 2: 1). Plant materials are cultivated in the artificial intelligence greenhouse on the Yuzhong campus of Lanzhou University, where the day and night temperature is 28 / 14°C, the relative air humidity is 30%, and the conventional management methods are adopted to cultivate until the flowering period. Select healthy plants with similar growth, and sample the roots, stems, leaves, flowers and tiller buds of the plants. After sampling, the samples are quick-frozen with liquid nitrogen, and then transferred to the ultra-low temperature refrigerator (-80℃) for storage for RNA extrac...
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