Cervinae activin A protein and preparation method and application thereof

Abstract

The invention relates to the field of molecular biology and particularly relates to a cervinae activin A protein and a preparation method and application thereof. The activin A protein is composed oftwo cervinae activin beta A subunits isolated from cervinae animals. The study on the activin A protein is still relatively rare in the cervinae. The preparation method acquires the isolated gene sequence, improves its high-purity protein expression method, provides research basis and novel ideas for related research and greatly increases the research and application prospect of the activin A protein.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a deer subfamily activin A protein and its preparation and application. Background technique [0002] Activin (Actvin, ACT), also known as activin, is a glycoprotein hormone firstly isolated and purified from porcine follicular fluid by Vale et al. and Ling et al. It is named after the inhibition of pituitary cell secretion of follicle stimulating hormone (Follicle Stimulating Hormone, FSH), which belongs to the transforming growth factor β superfamily member. Activin is mainly synthesized and secreted by ovarian granulosa cells and placenta. Because it has the biological activity of specifically promoting the secretion and synthesis of follicle stimulating hormone by pituitary cells, it is named activin. [0003] Both ACT and INH are dimers composed of two subunits. ACT is composed of β subunits, including homodimer ACTA (βA-βA) and ACTB (βB-βB) or heterodimer ACTAB (βA-βB). ...

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Problems solved by technology

[0004] However, there are still few domestic studies on ACTA in the subfamily Cervidae, and there is no report on ACTA protein and cDNA sequences in the subfamily Cervidae. and the research on the fecundity of deer subfamily will cause obstacles

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