SARS-CoV-2 susceptible gene combination and application in preparation of COVID-19 susceptible population screening kit

A susceptibility gene, gene combination technology, applied in the field of medical testing

Pending Publication Date: 2022-01-21
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no direct evidence to prove that people who carry a specific combination of genes will definitely be infected by a specific virus, but from a population level, it is widely recognized that people who carry specific genes have a higher probability of developing specific diseases

Method used

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  • SARS-CoV-2 susceptible gene combination and application in preparation of COVID-19 susceptible population screening kit
  • SARS-CoV-2 susceptible gene combination and application in preparation of COVID-19 susceptible population screening kit
  • SARS-CoV-2 susceptible gene combination and application in preparation of COVID-19 susceptible population screening kit

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Embodiment 1

[0031] In the previous work, the applicant speculated that there were certain susceptibility genes in the patients with the new crown. Therefore, the applicant conducted a genetic screening specifically for the genes of the Chinese population, selected blood samples from patients with the new crown and healthy people, and used the PCR-SSP method Screening the existing known alleles, we found more susceptibility risk genes for the new coronavirus. After strict comparison, we found that the existing gene combination (KIR2DL2 and HLA-C1 / C1) is the most common in the Chinese population. Representative risk genes.

[0032] The HLA-C gene family includes 15 genes, as shown in the following table:

[0033] Gene combinations associated with human SARS-CoV-2 virus susceptibility:

[0034] gene name gene bank no KIR2DL2 FJ188688 HLA-C*01 AM418559 HLA-C*02 MN509075 HLA-C*03 AJ298837 HLA-C*04 MH534947 HLA-C*05 LN879398 HLA-C*06 ...

Embodiment 2

[0054] Application of reagents for detecting human gene combinations in the preparation of human SARS-CoV-2 susceptibility gene screening kits:

[0055] The applicant conducted a retrospective study on the gene carrier status of 98 healthy volunteers not infected with SARS-CoV-2 and 257 COVID-19 patients.

[0056] According to the Novel Coronavirus Diagnosis and Treatment Guidelines (Eighth Edition) issued by the National Health Commission of China, COVID-19 patients were divided into a mild group of 99 and a severe group of 158. Among them, mild disease was defined as no respiratory symptoms, no lung imaging findings; or mild respiratory symptoms, radiological evidence of pneumonia. Severe cases are defined as SpO 2 ≤93% need oxygen support; or need heart-lung machine support for acute respiratory distress syndrome (ARDS).

[0057] 257 samples of blood cell DNA recovered from COVID-19 and 98 people not infected with SARS-CoV-2 were used to detect KIR2DL2 and HLA-C genes usi...

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Abstract

The invention belongs to the field of medical detection, and particularly relates to an SARS-CoV-2 susceptible gene combination and application in preparation of a COVID-19 susceptible population screening kit. The human gene combination disclosed by the invention is KIR2DL2 and HLA-C, and specifically is HLA-C*01, HLA-C*02, HLA-C*03, HLA-C*04, HLA-C*05, HLA-C*06, HLA-C*07, HLA-C*08, HLA-C*09, HLA-C*10, HLA-C*12, HLA-C*14, HLA-C*15, HLA-C*16 and HLA-C*17. At present, early-stage prediction related researches on the occurrence of SARS-CoV-2 virus infection and susceptible populations do not exist in the world. The kit provided by the invention has great application value in disease susceptible population.

Description

technical field [0001] The invention belongs to the field of medical detection, and in particular relates to a SARS-CoV-2 susceptibility gene combination and its application in the preparation of a COVID-19 susceptible population screening kit. Background technique [0002] The World Health Organization declared the coronavirus disease 2019 (COVID-19) outbreak a public health emergency on January 30, 2020 [1] . The causative agent of COVID-19 is coronaviruses (CoVs) (SARS-CoV-2), which belong to the Coronaviridae family and are characterized by positive-sense single-stranded ribonucleic acid (RNA). Coronavirus genomes are highly mutable, leading to gene drift and evasion of immune recognition [2] . [0003] In the study of SARS-CoV-2 infection, some familial disease characteristics were found, mainly in the direct family members with blood relationship, suggesting that the occurrence and development of the disease may be closely related to the immune genetic background of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12Q1/6881C12N15/11
CPCC12Q1/6883C12Q1/6881C12Q2600/156
Inventor 胡松孙宾莲刘钰晨舒细记彭倩万和杏刘小龙黄艺翟昊阳
Owner JIANGHAN UNIVERSITY
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