53 results about "Adenine dinucleotides" patented technology

The invention relates generally to the field of industrial microbiology and alcohol production. More specifically, the invention relates to the use of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) to improve butanol production. Butanol production can be improved by providing sufficient amounts of thiamine, biosynthetic precursors of thiamine, nicotinic acid, nicotinamid, nicotinic acid riboside, nicotinamid riboside, or other biosynthetic precursors of nicotine adenine dinucleotide (NAD) in the production media.

The present invention provides a novel L-glutamate oxidase, a gene encoding the enzyme, and a method for producing the enzyme. By use of a gene encoding the enzyme, L-glutamate oxidase can be readily prepared at low costs through a recombinant DNA technique. The novel L-glutamate oxidase has the following physicochemical properties: (A) action: catalyzing the following reaction: L-glutamic acid+O2+H2O→α-ketoglutaric acid+H2O2+NH3; (B) substrate specificity: being specific to L-glutamic acid; (C) molecular weight and subunit structure: molecular weight as determined through SDS-polyacrylamide gel electrophoresis of 70,000±6,000, molecular weight as determined through gel filtration of 140,000±10,000, and being a dimer formed of the same subunits having a molecular weight of 70,000±6,000; (D) optimum pH: around pH 6.0 to 8.5; (E) heat stability: being stable up to 60° C. at a pH of 7.4 for 30 minutes; and (F) coenzyme: flavin adenine dinucleotide (FAD).

The invention discloses a construction method of a saccharomyces cerevisiae strain with a high yield of isobutanol and relates to preparation of microorganism by a recombinant DNA (Deoxyribonucleic Acid) technology. According to the method, the saccharomyces cerevisiae strain with the high yield of isobutanol is constructed by reforming saccharomyces cerevisiae cells by using molecular biology and the gene recombination technology. The method comprises the following steps: S1, constructing plasmids of over-expressing ILV2 (Induced Leukemia Virus), ILV3, ILV5 and ZWF1 genes of saccharomyces cerevisiae; and S2, constructing a saccharomyces cerevisiae strain HBGDAL-103 with the high yield of isobutanol. The method disclosed by the invention overcomes the deficiency that further improvement of the yield of isobutanol is restricted by factors such as imbalance of a coenzyme NADPH / NADP<+> (Reduced Form of Nicotinamide-Adenine Dinucleotide Phosphate) / (Nicotinamide-Adenine Dinucleotide Phosphate<+>) from pyruvic acid to 2-one isovaleric acid due to over-expressed ILV2, ILV3 and ILV5 in the saccharomyces cerevisiae cells of yielding isobutanol in the prior art.

It is intended to highly efficiently produce a large amount of novel FAD-GDH capable of more accurately measuring a glucose level. A transformant transfected with a DNA encoding a flavin adenine dinucleotide-binding glucose dehydrogenase selected from the group consisting of (a) a DNA encoding an amino acid sequence represented by SEQ ID NO: 20; (b) a DNA composed of a base sequence represented by SEQ ID NO: 19; (c) a DNA having a base sequence homologous to the base sequence represented by SEQ ID NO: 19 and encoding a protein having a flavin adenine dinucleotide-binding glucose dehydrogenaseactivity; (d) a DNA encoding an amino acid sequence represented by SEQ ID NO: 34; (e) a DNA composed of a base sequence represented by SEQ ID NO: 33; and (f) a DNA having a base sequence homologous to the base sequence represented by SEQ ID NO: 33 and encoding a protein having a flavin adenine dinucleotide-binding glucose dehydrogenase activity is provided. Further, a method for producing a flavin adenine dinucleotide-binding glucose dehydrogenase using the transformant is provided.