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Carbon dioxide measurement kit

A technology of carbon dioxide and kits, applied in the field of kits, can solve the problems of poor opening stability of reagents, lack of opening stability, and decreased substrate concentration, and achieve the effects of good stability, simple operation, and convenient use

Inactive Publication Date: 2014-02-05
QINGDAO JINYANG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] In recent years, the technology of enzymatic determination of carbon dioxide has developed rapidly. It is suitable for automatic biochemical analyzers with fast detection speed and high accuracy. 2 The reaction occurs, resulting in the decrease of the substrate concentration and the poor opening stability of the reagent. At present, domestic research on solving the poor opening stability of the carbon dioxide determination reagent is also very lacking.

Method used

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Effect test

Embodiment 1

[0049] The carbon dioxide measurement kit of the present embodiment is a single reagent, comprising:

[0050] Tris-hydrochloric acid buffer: 50mM,

[0051] Glycerin: 10%,

[0052] Triton X-100: 0.1%,

[0053] Sodium azide: 0.1g / L,

[0054] Magnesium sulfate: 1mM,

[0055] 3-acetylpyridine adenine dinucleotide (reduced form): 2mM,

[0056] Phosphoenolpyruvate: 5g / L,

[0057] Phosphoenolpyruvate carboxylase: 50U / L,

[0058] Malate dehydrogenase: 1KU / L,

[0059] 3-acetylpyridine adenine dinucleotide (reduced) cyclic regeneration system:

[0060] (1) Glucose: 2g / L,

[0061] (2) Glucose dehydrogenase: 100U / L,

[0062] (3) 3-acetylpyridine adenine dinucleotide (reduced form): 1 mM.

[0063] After all the reagents are dissolved and prepared, place them overnight at 4°C, and divide them into bottles.

[0064] Mix the measured carbon dioxide sample and the reagent so that the volume ratio of the sample to the reagent is 1:100, mix evenly, react at 37°C for 1 minute, and dete...

Embodiment 2

[0071] The carbon dioxide measurement kit of the present embodiment is a single reagent, comprising:

[0072] Tris-hydrochloric acid buffer: 200mM,

[0073] Glycerin: 40%,

[0074] Dithiothreitol: 1mM,

[0075] Triton X-100: 5%,

[0076] Sodium azide: 5g / L,

[0077] Sodium sulfate: 20mM,

[0078] 3-acetylpyridine adenine dinucleotide (reduced form): 10mM,

[0079] Phosphoenolpyruvate: 50g / L,

[0080] Phosphoenolpyruvate carboxylase: 500U / L,

[0081] Malate dehydrogenase: 3KU / L,

[0082] 3-acetylpyridine adenine dinucleotide (reduced) cyclic regeneration system:

[0083] (1) Glucose 6 phosphate: 20g / L,

[0084] (2) Glucose 6-phosphate dehydrogenase: 1000U / L,

[0085] (3) 3-acetylpyridine adenine dinucleotide (oxidized form): 5 mM.

[0086] After all the reagents are dissolved and prepared, place them overnight at 4°C, and divide them into bottles.

[0087] Mix the measured carbon dioxide sample and the reagent so that the volume ratio of the sample to the reagent is...

Embodiment 3

[0095] The carbon dioxide measurement kit of the present embodiment is a single reagent, comprising:

[0096] CAPSO buffer: 200mM,

[0097] Glycerin: 40%,

[0098] Dithiothreitol: 1mM,

[0099] Bovine serum albumin: 5g / L,

[0100] Triton X-100: 1%,

[0101] PC-300: 0.1g / L,

[0102] Potassium sulfate: 5mM,

[0103] 3-acetylpyridine adenine dinucleotide (reduced form): 2mM,

[0104] Phosphoenolpyruvate: 5g / L,

[0105] Phosphoenolpyruvate carboxylase: 200U / L,

[0106] Malate dehydrogenase: 2KU / L,

[0107] 3-acetylpyridine adenine dinucleotide (reduced) cyclic regeneration system:

[0108] (1) Glucose: 10g / L,

[0109] (2) Glucose dehydrogenase: 500U / L,

[0110] (3) 3-acetylpyridine adenine dinucleotide (reduced form): 2 mM.

[0111] After all the reagents are dissolved and prepared, place them overnight at 4°C, and divide them into bottles.

[0112] Mix the measured carbon dioxide sample and the reagent so that the volume ratio of the sample to the reagent is 1:100, m...

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Abstract

The invention provides a carbon dioxide measurement kit. The carbon dioxide measurement kit mainly comprises the following components: 50 to 200mM of a buffer solution, 10 to 50% of a stabilizer, 0.1 to 5% of a surface active agent, 0.1 to 5g / L of a preservative, 1 to 20mM of a reaction accelerator, 2 to 10mM of 3-acetylpyridine adenine dinucleotide (reduction type), 5 to 50g / L of phosphoenolpyruvic acid, 50 to 500U / L of phosphoenolpyruvate carboxylase, and 1 to 3KU / L of malate dehydrogenase; a recycling system of 3-acetylpyridine adenine dinucleotide (reduction type) comprises (1) 2 to 20g / L of glucose substrate, (2) 100 to 2,000U / L of corresponding glucose dehydrogenase, and (3) 1 to 5mM of 3-acetylpyridine adenine dinucleotide. The carbon dioxide measurement kit is convenient to use and simple to operate; the components participating in coupling reaction are extra added, so that no pollution caused by endogenous and allogenic substances are introduced; the rate of the recycling system is below the main reaction rate, thus the main reaction cannot be interfered; the carbon dioxide measurement kit is high in stability and can be used for a long time.

Description

technical field [0001] The invention relates to a kit, in particular to a carbon dioxide measuring kit commonly used in the fields of medicine, food and environmental monitoring. Background technique [0002] Blood carbon dioxide (CO 2 ) content plays an important role in the regulation of acid-base balance in the human body, and the constant blood pH is an essential condition for maintaining life activities. In metabolic acidosis and respiratory alkalosis, the concentration of carbon dioxide in human serum (plasma) decreases; while in metabolic alkalosis and respiratory acidosis, the concentration of carbon dioxide in human serum (plasma) increases. Therefore, the determination of carbon dioxide in serum (plasma) plays an important role in clinical diagnosis and treatment observation. [0003] The determination methods of total carbon dioxide and bicarbonate can be divided into direct measurement and indirect calculation. Direct determination mainly includes measuring ga...

Claims

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Application Information

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IPC IPC(8): G01N33/52
CPCG01N33/52
Inventor 周玭温云飞
Owner QINGDAO JINYANG BIOTECH
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