The invention discloses a waterborne interference type pearly flashing paint for an automobile and a preparation method of the waterborne interference type pearly flashing paint. The waterborne interference type pearly flashing paint is prepared from the following components: mica pearl powder dispersion paste, waterborne polyurethane dispersoid, self-thickening acrylic emulsion, waterborne amidogen resin, a thickening agent, an amine neutralizer, a diluent and water. The waterborne interference type pearly flashing paint has scientific matching, the components are adjusted mutually, the drying speed is high, the construction performance is good, the requirements of 2C1B coating process on coating are met, costs are greatly saved, energy consumption is reduced, and the water-soluble interference type pearly flashing paint has the advantages of energy conservation and emission reduction. A paint film formed by the pearly flashing paint has gorgeous and gentle gloss, has pearly colors, is full of sense of beauty, does not have phenomena such as color difference, floating, seediness, dulling or poor pearl effect, has excellent water resistance and corrosion resistance performance andgood weather resistance performance, and meets the decorative requirements of limousines.