81results about How to "The test result is simple" patented technology

The invention discloses a full automatic biochip detection system belonging to the clinical detection field of multi-marker biochips. The system adopts modular structure and comprises an automatic sample processing module, a reaction washing module, a detection module, a Tip head storage module, a reagent storage module, a biochip storage module, a sample storage module, a system base, an electric control box and a computer, the electric control box and all the modules are operated under the control of software, so as to realize the automatic sampling, the sample adding, the reaction, the washing, the detection and other processes. All the modules can work independently and can also be combined together and matched for work. The full automatic biochip detection system can overcome the shortcomings of the existing biochip detector and reduce the errors caused by human operation, and has stable and reliable detection result and good flexibility, so that the full automatic biochip detection system can not only greatly improve the detection efficiency, but also can be conductive to the accuracy of the detection result; the system has simple operation, therefore, the system is not only applicable to the scientific research and the applications of laboratories and hospitals, but also can be used for large-scale sample screening of large-scale hospitals and blood stations.

The invention relates to a large-caliber high-gradient optical mirror surface on-line measuring system which comprises an optical fiber light source, a converging light path light split system, a replaceable measuring grating, a high-precision plane reflecting mirror, a large-caliber high-gradient optical mirror surface, a digital CCD (Charge Coupled Device) and a computer information processing system. In the invention, after light emitted out from a numerical aperture optical fiber light source passes through the measuring grating, a group of shadow fringes is generated, and after passing through the converging light path light split system and the high-precision plane reflecting mirror, the shadow fringes are projected onto the large-caliber high-gradient optical mirror surface and then reflected back by the large-caliber high-gradient optical mirror surface according to the original path; when the shadow fringes pass through the high-precision plane reflecting mirror and the converging light path light split system, another light path is generated, and a conjugate image of the light source is generated in the light path; after the conjugate image is acquired by the digital CCD, the conjugate image is transmitted to a computer and analyzed and processed by the computer information processing system; and the surface shape error of the large-caliber high-gradient optical mirror surface is worked out through comparing the deviations between the returned image and an ideal image.

The invention discloses a quick African swine fever virus detection card and application thereof, so as to solve the technical problem of hard African swine fever virus detection. Polyclonal antibodies PoAbI against African swine fever virus p30, P54 and p72 protein labeled with colloidal gold are absorbed on a colloidal gold labeling pad of the detection card; a detection membrane is provided with goat or rabbit anti-mouse IgG antibodies or a quality control line C printed by staphylococcus aureus SPA; and a detection line T printed by polyclonal antibodies PoAbII against African swine fevervirus p30, P54 and p72 protein is also arranged. The invention also discloses a using method for the quick African swine fever virus detection card. The method comprises steps: a to-be-detected sampleis acquired, and PBS or water is added for suspension or grinding; the sample is dripped to the sample loading end of the quick African swine fever virus detection card; and horizontal placing is carried out to observe a result. The quick African swine fever virus detection card adopts a double antibody sandwich method, and has the advantages of strong specificity, high sensitivity, high detection efficiency, high efficiency and practicability, and important practical application value.

The invention relates to a servo motor test bench and a test method. The invention aims to provide a servo motor test bench which has high detection precision and is not related to the rotating speed of a motor, and a test method. The servo motor test bench comprises a test bench and a load motor mounted on the test bench; a rotor of the load motor is connected with a rotor of a to-be-tested servo motor; a stator of the to-be-tested servo motor is fixedly connected with the test bench; a stator of the load motor is movably connected with the test bench; and the test bench is also provided with a pressure sensor for detecting the rotation moment of the stator of the load motor. The servo motor test bench has the advantages that the torque change process of the to-be-tested servo motor can be detected when the to-be-tested servo motor is subjected to load change, the detection precision is not affected by the given velocity value, the requirement for the torque detection of the large-range speed value of the servo motor can be met, and the detection results are visual and brief.

The invention discloses a kit and a method for detecting the methylation level of liver cancer risk gene TSPYL5, and belongs to the field of biotechnologies. The kit for detecting the methylation level of the TSPYL5 comprises a primer pair A used for an MSRE-qPCR method or a primer pair B used for a BSP method, wherein the primer sequence is shown in SEQ ID NO. 1-22. According to the kit and the method disclosed by the invention, the relevance between the methylation of the TSPYL5 gene and liver cancer occurrence is found, and the methylation loci of the TSPYL5 are authenticated; the methylation method for the TSPYL5 by virtue of fluorescent quantitative PCR is established by combining specific methylation sensitive restriction endonuclease with the specific primers; the methylation level of the TSPYL5 is detected through the kit and the method based on MSRE-qPCR, the sensitivity is up to 83.9%, and the specificity is up to 93.25%.

The invention discloses a kit for detecting pig pseudorabies. The kit comprises an assay plate coated with pig pseudorabies virus gE antigen, a rabbit anti-pig IgG antibody marked by HRP (horseradish peroxidase), fast coating buffer, fast blocking buffer, sample diluents, TMB substrate, stop buffer, 20 times wash solution, negative control, and a positive control. Through adopting pig pseudorabies virus specificity gE antigen with high purity and high activity expressed through gene engineering to coat the assay plate, and through adopting HRP-conjugate of rabbit anti-pig IgG monoclonal antibody containing HRP, the kit for detecting pig pseudorabies, provided by the invention, has the advantages of strong specificity, high sensibility, less susceptibility to misjudge of false positive or false negative and the like; moreover, the kit is simple in structure, low in detection cost, convenient and fast in operation and strong in timeliness, has a wide market prospect, and can create larger social benefits.

The invention relates to a chemical etching measurement method for optical element sub surface damages, an auxiliary experiment device and a test method. In the chemical etching measurement method, through measuring the roughness of a sample during the etching process, an evolutional relation curve for roughness outlines along with the etching time is drawn, and a sub surface damage depth value isthus obtained. The chemical etching auxiliary device is used to realize etching, cleaning and drying on the optical element, an ultrasonic vibration instrument is added during the cleaning process toassist cleaning, and a hair drier and a motor are added during the drying process to assist drying. The sub surface damage depth can be quickly and accurately measured; a set of etching auxiliary device is designed, the device is simple in operation, high in efficiency and low in danger, sediments generated during the etching process can be effectively reduced, and influences on etching and measurement are greatly reduced.

The invention discloses a test strip that is used for detecting antibodies of porcine parvovirus and porcine encephalitis B virus in one step, which comprises a supporting layer, an adsorptive layer and a protecting layer, and the adsorptive layer consists of a sample fiber layer, an adsorptive gold mark fiber layer, a fibrin membranous layer and a water absorptive material layer sequentially from a testing end; the adsorptive gold mark fiber layer adsorbs any one or two from antigen liquids of gold colloid marked SPA or gold colloid marked pure PPV and JEV; a detecting print and a contrast print are arranged on the fibrin membranous layer, the detecting print is printed with any one or two from the purified PPV and JEV antigen liquids, and the contrast print is printed with an IgG solution of ovine or rabbit anti-SPA or an IgG solution of any one or two viruses from ovine or rabbit anti-PPV and anti-JEV. The test strip of the invention has strong detecting specificity and high sensitivity, avoids additional equipment and reagents, can be operated by every person, and is particularly applicable to the quick specific antibody detection of porcine diseases on the spot.

The invention provides an N gene specific primer pair, a method and a kit for detecting resistance of tobacco to a TMV. The specific primer pair comprises an upstream primer and a downstream primer, wherein the nucleotide sequence of the upstream primer is as shown in SEQ ID NO:1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO:2. The method comprises the following steps: designing a pair of specific primers in a tobacco N gene sequence specific zone; with the DNA of a to-be-detected tobacco variety as a template, performing PCR amplification on the N gene specific primer pair; and detecting a PCR amplification product by electrophoresis and judging whether the PCR amplification product contains a 865bp characteristic band, wherein if so, the tobacco has resistance to TMV, and otherwise, the tobacco does not have N gene mediated resistance to TMV. The method has the advantages of reliable result, high detection speed and the like and is simple to operate, the breeding process of TMV-resisting breeding materials can be greatly accelerated, the breeding period is shortened and the breeding efficiency is enhanced.

The device consists of multiple immediate static electricity detector, an OR-logic circuit and a general actuator as immediate static electricity detector is equipped with a RC-oscillator, pulse width regulation controller, voltage comparator, driving amplifier and personal warner. The automatic immediate static electricity warning device can check out at any time whether static electricity collection component is accessorized on operator at each production line or not and it can launch alarm in both modes of sound and light if it finds that something is wrong.

The invention particularly relates to florfenicol on-site test paper, and preparation and use methods thereof and belongs to the field of immunology. The test paper includes anti-florfenicol amine secreted from hybridoma FFA-C which is assigned the accession number CCTCC-C201575, a monoclonal antibody C marked by colloidal gold and a carrier plate having adhesive sticker, wherein a sample adding end water absorption layer and a hand holding end water absorption layer are formed at two ends of the carrier plate respectively. A test layer is arranged on the middle of the carrier plate. A glass fiber layer block, on which the monoclonal antibody C marked by colloidal gold is supported, is arranged at the boundary between the test layer and the sample adding end water absorption layer. One end of the glass fiber layer block is arranged under the sample adding end water absorption layer and the other end of the glass fiber layer block is arranged above the test layer. A test line and a quality control line are formed on the test layer extended from the glass fiber layer block, wherein the test line and the quality control line are respectively coated by an anti-florfenicol amine polyclonal antibody and sheep-anti mouse IgG. The test paper quickly, simply and accurately detects the florfenicol and the metabolite, florfenicol amine, thereof on site, and can be used for crude screening test of drug residue of the florfenicol.

The invention relates to a detection method for the pathogenicity of maize bacterial wilt source fusarium in a maize seedling stage. The detection method comprises the following steps of: preparing maize infected by the maize bacterial wilt source fusarium, designing and synthesizing a primer, extracting the DNA (deoxyribonucleic acid) of the maize infected by the maize bacterial wilt source fusarium, amplifying and separating target genes, separating amplified end-products, carrying out sequencing evaluation, comparing in a Genbank, and determining that the maize is infected by the maize bacterial wilt source fusarium. The detection method for the pathogenicity of the maize bacterial wilt source fusarium in the maize seedling stage has the advantages that an identification method is simple in operation, an evaluation result is obtained rapidly and accurate, and the detection method can be applied to the actual production.

The invention designs an internal reference detection system, and concretely relates to an internal reference detection system for the isothermal nucleic acid amplification reaction, a kit containing the internal reference detection system and an application of the system. The internal reference detection system is used for detecting the false negative reaction in the isothermal the nucleic acid amplification reaction, and comprises template DNA with a hairpin structure, a specific primer 1 and a specific primer 2, wherein the specific primer 1 is complementary with the sequence of a ring in the hairpin structure, and the specific primer 2 is complementary with the 3'-terminal sequence at the stem in the hairpin structure. The invention also relates to the kit of the internal reference detection system for the isothermal nucleic acid amplification reaction, and the kit comprises a strand displacement-function DNA polymerase-mediated isothermal nucleic acid amplification system and an internal reference detection system. The internal reference detection system of the invention has the advantages of exquisite design, sensitive reaction and suitableness for the large-scale popularization and use.

The invention relates to a method for checking cucumber fusarium axysporum molecules. The invention solves the problems that the specificity of fungi general primers is not strong and the method is complicated by using the PCR-RFLP method and the nested PCR method needs two steps for amplification and is easy to pollute environment in the existing methods for checking the cucumber fusarium axysporum moleculars. The method for checking the cucumber fusarium axysporum moleculars comprises the following steps: (1) primers Fc-1 and Fc-2; (2) extracting ailing sample genome DNA to perform the PCR amplification; and (3) performing electrophoresis for a PCR amplification product and analyzing the product by a gel imaging system, wherein if a special strip appears at the position of 315bp, the ailing sample is provided with the cucumber fusarium axysporum, therefore the checking step is finished. The primers Fc-1 and Fc-2 have high specificity and sensitivity; and the checking result can be obtained in 2 hours with only one step, and the method is fast, simple and not easy to pollute environment.

The invention provides a method for detecting bactrocera dorsalis based on a visual LAMP (loop-mediated isothermal amplification) technology, which comprises the following steps: 1) designing a specific LAMP primer group by taking a section containing a polymorphic variation site in a bactrocera dorsalis mitochondrial COI gene as a target gene; (2) carrying out LAMP reaction by using the specific LAMP primer group and taking the whole genome DNA of a sample to be detected as a substrate; and 3) after the LAMP reaction is finished, judging whether the sample to be detected is the bactrocera dorsalis or not according to the color development result of the nucleic acid dye for detection. The invention also provides a corresponding primer group and application thereof. The method and the primer group provided by the invention can accurately detect bactrocera dorsalis in 73 species of bactrocera, have high sensitivity, and can be used for visual rapid detection of a detected material in biological quarantine.

The invention discloses a method for sampling detection of the bonding strength of an exterior facing pre-buried stone. The method comprises the following steps: superposing the center of a sample region, the center of an anchoring part and the center of a drawing tester, removing adverse factors for eccentric loading, pulling a facing stone sample from a concrete wall body through the drawing tester, and calculating the bonding strength of the exterior facing pre-buried stone according to a maximum drawing force value and a sectional area. The method is easy and convenient to operate and lowin cost and can directly and accurately reflect the bonding strength of the exterior facing pre-buried stone and provide a basis for quality detection and authentication; meanwhile, the adverse factors for eccentric loading are eliminated, and a detection result is reasonable and reliable; furthermore, the method can be applicable to four conventional anchoring part forms, and fills the blank in the aspect of sampling detection of the bonding strength test of the exterior facing pre-buried stone; a practical and quantifiable method is provided for guaranteeing the bonding quality of the exterior facing pre-buried stone; and the used drawing tester is simple in structure, low in cost, accurate in detection result and favorable for being popularized and applied.

The invention discloses a toxoplasma gondii dense granular protein GRA1. A nucleotide sequence for encoding the protein is shown as SEQ ID NO:1. The invention further discloses an application of the dense granular protein GRA1 to the preparation of an enzyme-linked immunoassay detecting kit for treating toxoplasmosis and an enzyme-linked immunoassay method for detecting a sheep toxoplasma gondii IgG antibody. The method has the advantages such as high sensitivity, strong specificity, good accuracy as well as high speed, simplicity and convenience when being used for detecting the toxoplasmosis.

The invention provides a kit for detecting cadmium ions based on a direct competition enzyme-linked immunoadsordent assay. The kit comprises a detection plate coated with a goat anti-mouse IgG secondary antibody, a Cd-ITCBE chelate hapten with the mass concentration of 20mu g / mL to 50mu g / mL, an anti-cadmium ion monoclonal antibody solution with the mass concentration of 10mu g / mL to 20mu g / mL, 1.0mol / L to 3.0mol / L of a stopping solution, a sample diluting solution, a substrate color developing solution, a washing solution, a cadmium ion standard product solution and an EDTA (Ethylene Diamine Tetraacetic Acid) treatment solution with the mass concentration of 10mg / mL to 20mg / mL, wherein the detection plate is sealed and packaged in vacuum; the coating concentration of the goat anti-mouse IgG secondary antibody is 50mu g / mL to 200mu g / mL; the anti-cadmium ion monoclonal antibody solution is prepared from Cd-ITCBE-cBSA immunogen immunized Balb / C mice. The kit provided by the invention has the characteristics of high sensitivity and high specificity and also has the advantage of short detection time.

The invention relates to an initiative detection device for an oil pollution degree. The device mainly comprises an oil inlet end cover, a circular shell, a 15mu m grain filtering piston, a 5mu m grain filtering piston, reset springs, a spoiler, throttle valve rods, an oil outlet end cover and a pressure sensor, wherein the 15mu m grain filtering piston, the 5mu m grain filtering piston and the spoiler are arranged in the circular shell and are connected through two throttle valve rods; two conical valve elements are arranged on the throttle valve rods, and the throttle valve rods are respectively matched with valve holes of the corresponding pistons; and the reset springs are arranged between the 15mu m grain filtering piston and the spoiler and between the 5mu m grain filtering piston and the oil outlet end cover. The obvious change time interval of the pressure value of the pressure sensor is acquired according to the magnitude of the oil pollution degree, and the obvious change time interval is compared with a database result, so that the pollution degree of the to-be-detected oil can be acquired. According to the invention, oil and grain pollutants can be uniformly mixed, filtering cloth is not blocked, the detection operation is simple, the data is reliable and effective, and personal errors are reduced.

The invention discloses a spot welding strength detection mechanism. The detection mechanism comprises a detection table surface; an air cylinder is arranged on the detection table surface; the lowerend of the air cylinder is movably connected with a pressure head; a detection base fixed on the detection table surface is arranged below the air cylinder; the detection base has an upward opening; and the size of the opening of the detection base is smaller than the size of a metal plate I at an upper layer of a to-be-detected metal workpiece and is slightly larger than that of a metal plate IIat a lower layer of the to-be-detected metal workpiece. According to the detection mechanism, by adopting an air pressure of the preset air cylinder, and using the pressure head connected with the aircylinder to apply a force to welded lower layer metal, whether the welding spots of the lower layer metal and upper layer metal have potential failure or not is detected, so that unqualified welded parts are removed, and the potential failure risk caused by the fact that the welding spots of spot welding are not visible and cannot be detected is avoided; and meanwhile, the requirements of semi-automatic operation on operators are relatively low, a detection result is simple and intuitive, and the detection rate is high.

The invention discloses a field mouse babesia RPA molecular detection method. The field mouse babesia RPA molecular detection method comprises the following steps: designing a pair of specific primersand probes according to field mouse babesia mitochondria COX I gene sequence, wherein the nucleotide sequences of the primer pair is shown as SEQ ID NO: 2 to SEQ ID NO: 4 respectively; then extracting total DNA inside a dog blood sample to carry out recombinase combined enzymatic amplification reaction; and finally adopting a lateral flow analysis test paper strip to carry out detection on an amplification product. The method is high in sensitivity and high in specification, and field mouse babesia can be identified rapidly.

The invention discloses a real-time detection system and method for nucleic acid of pathogens. The system comprises: a lysis buffer, which is used for carrying out inactivation lysis on a to-be-detected sample to release RNA; a RT premixed solution, which is used for reversely transcribing RNA in a sample into DNA; a RPA premixed solution, which is used for carrying out RPA reaction on the sample treated by the RT premixed solution so as to realize amplification of DNA in the sample; a CRISPR reaction premixed solution, which is used for carrying out specific cleavage reaction on the sample treated by the RPA premixed solution; and colloidal gold test paper, which is used for developing the sample treated by the CRISPR reaction premixed solution so as to confirm whether the sample contains nucleic acid of pathogens or not. According to the above design scheme, a RPA method, a CRISPR method and a colloidal gold method are coupled, so the real-time detection system has the advantages of simplicity, sensitivity and specificity at the same time, does not need instruments and equipment, and is a real-time detection system capable of quickly obtaining a result through simple operation at room temperature.

The invention discloses an LAMP primer set for quickly detecting pseudomonas fluorescens producing thermostable lipase in raw milk and a method and belongs to the field of food safety inspection, andbelongs to the field of food safety detection. By taking a pseudomonas fluorescens lipase gene as a target gene, a set of specific detection primer set and an LAMP detection system and a reaction condition which are suitable for the primer set are designed and screened. A conventional detection method is long in time and consumes both manpower and physical materials. The adopted method is high insensitivity, strong in specificity and short in time; the cost is low, the operation process is simple, and a PCR instrument or an electrophoresis apparatus is not needed in the reaction process; andany instrument is not needed in the result judgment aspect. The shortcomings of the conventional method are overcome to a certain degree. The LAMP primer set and the method have significance on quickly detecting the quality of the raw milk. The adopted method is suitable for bed-layer on-line monitoring and field detection.

The invention belongs to the field of biotechnology detection, and particularly provides a Prader-Willi Syndrome detection kit and a use method thereof. According to the present invention, snO-lncRNA1, snO-lncRNA2, snO-lncRNA3, snO-lncRNA4 and snO-lncRNA5 are extremely highly expressed in human embryonic stem cells, and abnormally stable, so that the detection kit prepared by taking snO-lncRNA1, snO-lncRNA2, snO-lncRNA3, snO-lncRNA4 and snO-lncRNA5 as detection genes can detect samples such as common blood, saliva, urine and the like so as to provide guarantee for realizing non-invasive examination of Prader-Willi Syndrome; the use method is simple to operate and has low requirements on instruments and equipment; the input amount of the required sample is small, and the sample source is highly inclusive; the kit has the characteristics of high sensitivity, high specificity and the like; the detection result is visual, simple and easy to understand; and by using the detection method, Prader-Willi Syndrome can be detected in a short time.

The invention provides a device and method for accurate leak checking on blast furnace tuyere small sleeves, and belongs to the technical field of blast furnace inspection tools and methods. The device and method are used for leak checking on blast furnace tuyere small sleeves. According to the technical scheme, a water inlet pipe chuck front connecting part is connected with one end of an unfastened water inlet pipe chuck trough front threads, a water inlet pipe chuck rear connecting part is connected with the other end of the unfastened water inlet pipe chuck through rear threads, and a steam trap is installed between the water inlet pipe chuck front connecting part and the water inlet pipe chuck rear connecting part. According to the checking method, the characteristic that the potassium permanganate changes the color after dissolving in water is utilized, which sleeve is leaking water can be determined according to the changes of the color of leaking water, and the results of leak checking are visual and accurate. The device is simple in structure and convenient to operate. The device and the method are the pioneering technologies for checking leak of blast furnace tuyere small sleeves, the problem, which is not effectively solved for a long time, that whether water leak happens cannot be judged is solved with the simple checking device and method, the problem that in the prior art, the sleeve which is leaking cannot be accurately positioned is further solved, and therefore, the device and the method have completely positive practical effect on production.

The invention discloses a zinc complex, synthesis thereof and a method for recognizing Fe < 3 + > ions in fluorescence. The method comprises the following steps: S1, synthesis of a complex [Zn (3-bpam) (6-HNA) 2]; and S2, the recognition performance of the complex [Zn (3-bpam) (6-HNA) 2] on cations. Compared with traditional detection methods such as atomic absorption spectrometry, inductively coupled plasma and electrochemical methods, the obtained chemical sensor has the advantages of being high in sensitivity, convenient to operate, short in response time, capable of achieving real-time monitoring, low in cost and the like, and the obtained chemical sensor is based on the Fe < 3 + > ion fluorescence enhancement principle. Therefore, the problem that a detection result is distorted due to inaccurate light-emitting signals or wrong response to sensing signals of a traditional fluorescence quenching sensor is solved, Fe < 3 + > ions are easier to recognize, and an accurate detection result is obtained.

The invention provides a kit for detecting lead ions based on a direct competition enzyme-linked immunoadsordent assay. The kit comprises a detection plate coated with a goat anti-mouse IgG secondary antibody, a Pb-ITCBE chelate hapten with the mass concentration of 20mu g / mL to 50mu g / mL, an anti-lead ion monoclonal antibody solution with the mass concentration of 10mu g / mL to 20mu g / mL, 1.0mol / L to 3.0mol / L of a stopping solution, a sample diluting solution, a substrate color developing solution, a washing solution, a lead ion standard product solution and an EDTA (Ethylene Diamine Tetraacetic Acid) treatment solution with the mass concentration of 10mg / mL to 20mg / mL, wherein the detection plate is sealed and packaged in vacuum; the coating concentration of the goat anti-mouse IgG secondary antibody is 50mu g / mL to 200mu g / mL; the anti-lead ion monoclonal antibody solution is prepared from Pb-ITCBE-cBSA immunogen immunized Balb / C mice. The kit provided by the invention has the characteristics of high sensitivity and high specificity and also has the advantage of short detection time.

The invention discloses a kit for detecting pig pseudorabies. The kit comprises an assay plate coated with pig pseudorabies virus gE antigen, a rabbit anti-pig IgG antibody marked by HRP (horseradish peroxidase), fast coating buffer, fast blocking buffer, sample diluents, TMB substrate, stop buffer, 20 times wash solution, negative control, and a positive control. Through adopting pig pseudorabies virus specificity gE antigen with high purity and high activity expressed through gene engineering to coat the assay plate, and through adopting HRP-conjugate of rabbit anti-pig IgG monoclonal antibody containing HRP, the kit for detecting pig pseudorabies, provided by the invention, has the advantages of strong specificity, high sensibility, less susceptibility to misjudge of false positive or false negative and the like; moreover, the kit is simple in structure, low in detection cost, convenient and fast in operation and strong in timeliness, has a wide market prospect, and can create larger social benefits.