An automatic test method for the movement, learning and memory powers of mouse after stressed includes such steps as fixing a mouse to a experiment table with annular screen and camera, aligning the camera with the eyeball of mouse, focusing to obtain a clear eyeball image, rotating said annular screen to capture the image information of eyeball, inputting it to a computing unit, storing and processing the information to obtain the boundary information and central position of pupil, coinciding the circular boundary curve with the created pupil boundary, repeatedly recording the Nth central position of pupil and the position of a point on created pupil boundary at time intervals, and calculating the variation. Speed and amplitude of pupil within time T is calculated.