The invention relates to a Hi-C high-
throughput sequencing
library building method suitable for watermifoil. The method includes the steps of firstly, performing
formaldehyde crosslinking on a watermifoil sample to obtain a crosslinking material; secondly, performing
cell lysis to release
chromatin; thirdly, collecting the
chromatin, and sequentially subjecting the
chromatin to SDS deactivation endogenous
enzyme and MboI
endonuclease digestion breaking to obtain a material after
digestion; fourthly, performing
tail end filling to obtain
tail-end-filled
DNA, and performing intramolecular connection on the
tail-end-filled
DNA; fifthly, decrosslinking the intramolecular connection material and purifying
DNA, and performing DNA breaking to form fragments beneficial to sequencing; seventhly, building a Hi-C
library for high-
throughput sequencing; eighthly, specifically capturing
biotin marked DNA fragments, and performing amplification; ninthly, performing
library quality inspection. The method has the advantages that the appropriate
cell breaking method,
formaldehyde crosslinking condition, endogenous
enzyme deactivation condition and appropriate tool enzymes are designed according tothe special structure of watermifoil cells, the problem that the
cell wall structure of the watermifoil affects a Hi-C experiment, Hi-C high-
throughput sequencing library building of the watermifoil is achieved, and the application range of the Hi-C technology is expanded.