The invention relates to a healthcare wine for nourishing yin, tonifying yang, invigorating the middle-warmer and benefiting qi and a preparing method thereof. The healthcare wine is prepared by adding a grain wine into traditional Chinese medicine raw materials comprising barbary wolfberry fruit, Chinese date, grosvenor momordica fruit, saussurea involucrate, red ginseng, cogon grass rhizome, centella asiatica, common yam rhizome, honey, longan pulp, young pigeon, and the like, and dipping. The traditional Chinese medicines are derived from the nature and are low in toxic and side effects for human liver and low in anaphylaxis, the wine is a water-cereal substance and is capable of unblocking blood vessels, activating blood circulation to remove stasis and tonifying spleen and stomach, and reasonable compatibility of the traditional Chinese medicines and the wine can regulate human body function balance by utilization of functions of the traditional Chinese medicines and the wine. The healthcare wine is capable of nourishing yin, tonifying yang, invigorating the middle-warmer, benefiting qi and regulating spleen and stomach, has obvious healthcare effects for deficiency of yin in the liver and kidney, soreness and weakness of waist and knees, dizziness, insomnia and dreaminess, and the like, has a sweet, mild and smooth taste, and is a good healthcare product for people of all ages.