The invention relates to a method for preparing sodium gluconate through adopting a semicontinuous fermentation technology. The method comprises the following steps: 1, putting a fermentation medium into a fermentation tank, accessing an Aspergillus niger spore liquid, and carrying out temperature maintenance culture; 2, discharging 20-50% of the obtained culture solution, and simultaneously supplementing the fresh medium which is isometric to the discharged culture solution to culture; 3, circulatingly carrying out step 2; and 4, discharging the obtained material from the tank. Compared with traditional fermentation technologies, the preparation method of the invention, which adopts the fermentation medium with the sugar concentration of 5-10% higher than sugar concentrations of traditional fermentation mediums, has the advantages of improvement of the content of sodium gluconate in the total fermentation liquid, reduction of the steam disinfection frequency of the fermentation tank and a pipeline thereof, steam consumption reduction, strong production continuity, guarantee of the uniformity and the continuity of post-extraction production, and finished product quality stability guarantee.