36results about How to "Improve the conversion rate of enzymatic hydrolysis" patented technology

The invention discloses a combined preprocessing method of lignocellulose. The lignocellulose is arranged in a recycle reactor after being crushed, and dilute acid is injected; a recycle pump starts up, the circular reaction is acted under the temperature ranging from 50 DEG C to 200 DEG C, and the obtained hydrolysate is disacidified and then is used for ferment after the reaction is finished; the surplus lignocellulose in the recycle reactor is put in a globe mill for ball milling through adding lye after the surplus lignocellulose is washed, and the surplus lignocellulose is used in subsequent zymohydrolysis after ball milling and alkali removing. The invention effectively increases the hemicellulose percent conversion, the lignin removal rate and the cellulose zymohydrolysis percent conversion in the lignocellulose, reduces fermentation inhibition products, and reduces the enzyme usage when in zymohydrolysis, shortens the reaction time and increases the sugar consistence at the same time; in addition, the invention can set the water consumption during the preprocessing according to the needs of subsequent ferment consistence.

The invention discloses a method for preprocessing lignocellulose, which comprises: installing the lignocellulose into a recycle reactor, injecting diluted acid, opening a circulating pump to carry out cyclic reaction under the temperature of 50DEG C-200DEG C, acid-removing and filtering hydrolyzate which is obtained after finishing the reaction, and using the hydrolyzate to ferment. The invention also discloses a system which is applied in the method. The method of the invention can effectively increase the conversion rate of hemicellulose and the conversion rate of cellulase hydrolysis in the lignocellulose, fermentation inhibiting products are reduced, simultaneously, the time for preprocessing also can be shortened, and the energy consumption is lowered.

The invention relates to a comprehensive utilization method of lignocellulose biomass. The method comprises the following steps of (a) performing acid hydrolysis on the lignocellulose biomass to obtain a pentose solution and acid hydrolysis residues after separation; (b) treating the acid hydrolysis residues with an alkali solution so as to extract alkali lignin; (c) performing enzymatic hydrolysis on the alkali hydrolysis residues obtained by alkali solution treatment in the step (b) by using cellulose so as to obtain a glucose solution and enzymatic hydrolysis residues, wherein the cellulose is obtained by culturing a strain of penicillium, the penicillium is named as penicillium recumbent PD-G3-08; and (d) returning the enzymatic hydrolysis residues obtained by alkali solution treatment to the step (b) so as to perform alkali solution treatment, or firstly the enzymatic hydrolysis residues with fresh acid hydrolysis residues and then carrying out the alkali solution treatment of the step (b), performing the step (c) and the step (d) successively, repeating such process, and further extracting the lignin and performing cellulose enzymatic hydrolysis. The above method increases conversion rate of cellulose enzymatic hydrolysis and realizes comprehensive utilization of the lignocellulose biomass resources.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing acid hydrolysis of the lignocellulose biomass, separating to obtain a pentose solution and acid hydrolysis residues; (b) processing the acid hydrolysis residues by using an alkaline solution so as to extract alkaline lignin; (c) performing enzymatic hydrolysis of the alkaline hydrolysis residues obtained by alkaline solution processing in step (b) by using cellulase to obtain a glucose solution and enzymatic hydrolysis residues; (d) after step (c) is complete, returning the obtained enzymatic hydrolysis residues to step (b) for alkaline solution processing, or combining the enzymatic hydrolysis residues with new acid hydrolysis residues and performing alkaline solution processing in step (b), then orderly performing steps (c) and (d), cycling the steps so as to further extract lignin and to perform enzymatic hydrolysis of the celluose. The method improves the conversion rate of cellulose enzymatic hydrolysis, and realizes comprehensive resource utilization of lignocellulose biomass.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing alkaline hydrolysis processing; (b) performing acid hydrolysis of the alkaline hydrolysis residues, separating to obtain a pentose solution and acid hydrolysis residues; (c) performing enzymatic hydrolysis of the acid hydrolysis residues by using cellulase to obtain a glucose solution and enzymatic hydrolysis residues; (d) returning the enzymatic hydrolysis residues to step (b) for acid hydrolysis, or combining the enzymatic hydrolysis residues with new alkaline hydrolysis residues and performing acid hydrolysis processing in step (b), then orderly performing steps (c) and (d), cycling the steps so as to further convert the hemicelluloses into pentose and to perform enzymatic hydrolysis of the celluose into glucose. The method realizes the maximum resource utilization of lignocellulose biomass.

The invention relates to a herba epimedii total flavonoid enzymatic product. Herba epimedii total flavonoid is generated through immobilized helicase, and the enzymatic product has higher antineoplastic activity than the herba epimedii total flavonoid archetype and free enzyme enzymatic product and has high oral absorptivity. The invention further discloses a preparing method of the enzymatic product. The immobilized enzyme is used for enzymolysis of herba epimedii total flavonoid, selected helicase is high in combining efficiency with a fixed carrier and high in stability, the enzymolysis conversion rate is large, the immobilized enzyme can be used circularly, resources can be saved, and green production is achieved. The invention further relates to application of the enzymatic product in antitumous effect, and the herba epimedii total flavonoid enzymatic product obtained through the technical scheme has a remarkable anti-proliferative effect on the human lung cancer cell A549, liver cancer HepG2 cell, breast cancer MCF-7 cell, cervical cancer Hela cell and can be used for treating the lung cancer, liver cancer, breast cancer and cervical cancer.

The invention discloses a method for preparing chitooligosaccharide, which comprises the following steps of: firstly, pretreating chitin to obtain nano chitin, and then hydrolyzing the nano chitin by using chitinase SbChiAJ143 to obtain the chitooligosaccharide; wherein the amino acid sequence of the chitinase SbChiAJ143 is as shown in SEQ ID NO. 1. The mode of pretreating the chitin to obtain the nano chitin is that a grinding machine is used for grinding a chitin aqueous solution. The method for preparing chitooligosaccharide combines a physical grinding method and an enzyme method, and is high in efficiency. Compared with a method for preparing chitooligosaccharide through single enzymatic hydrolysis of colloid chitin, the method has the advantages that no chemical reagent is used in chitin treatment, the cost is saved, the steps are simplified, and environmental pollution is avoided. The method for preparing chitooligosaccharide is green, environment-friendly and efficient, and has good industrial application potential.

The invention provides a biomass processing method. Ammonia water is adopted in preprocessing of biomass raw materials for separating lignin from cellulose and hemicellulose, and a certain amount of environmentally-friendly auxiliary agents is added to increase the removal rate of the lignin and enhance the subsequent conversion efficiency of an obtained solid substrate. The preprocessed solid substrate is rich in the cellulose and the hemicellulose, and can be hydrolyzed to monosaccharides and used for the fermentation of ethyl alcohol and other products. A preprocessing liquid obtained by the method is rich in lignin degradation products and a small amount of hemicellulose degradation products, and the lignin degradation products and the hemicellulose degradation products can be recycledby acid precipitation, centrifugation or membrane separation and other techniques. Most of the ammonia water and the auxiliary agents used in the preprocessing are in the preprocessing liquid, can beseparated by waste steam recovery, reduced pressure distillation and other methods, and can be reused for biomass preprocessing. By adopting the preprocessing method, the preprocessing effect of theammonia water on biomass is obviously improved by the addition of the small amount of low-cost auxiliary agents, and the preprocessing method has a relatively good application prospect.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing N-stage acid hydrolysis of lignocellulose biomass, wherein the concentrations of the acid solutions decrease gradually from the first stage to the Nth stage by 10-40% of the concentration of the first stage acid solution,, and N is an integer more than or equal to 2; (b) processing the acid hydrolysis residues by using an alkaline solution so as to extract alkaline lignin; (c) performing enzymatic hydrolysis of the alkaline hydrolysis residues obtained by alkaline solution processing in step (b) by using cellulase to obtain a glucose solution and enzymatic hydrolysis residues; (d) after step (c) is complete, returning the obtained enzymatic hydrolysis residues to step (b) for alkaline solution processing, or combining the enzymatic hydrolysis residues with new acid hydrolysis residues and performing alkaline solution processing in step (b), then orderly performing steps (c) and (d), cycling the steps so as to further extract lignin and to perform enzymatic hydrolysis of the celluose. The method realizes the maximum resource utilization of lignocellulose biomass.

The invention relates to a comprehensive utilization method of lignocellulose biomass. The method comprises the following steps of (a) performing acid hydrolysis on the lignocellulose biomass to obtain a pentose solution and acid hydrolysis residues after separation; (b) treating the acid hydrolysis residues with an alkali solution so as to extract alkali lignin; and (c) performing enzymatic hydrolysis on the alkali hydrolysis residues obtained by alkali solution treatment in the step (b) by using cellulose so as to obtain a solution with glucose as a main component, wherein the cellulose is obtained by culturing a strain of penicillium, the penicillium is named as penicillium recumbent PD-G3-08 which is preserved in Wuhan University, China Center for Type Culture Collection, with a preservation number of CCTCC M 2011195. The above method increases conversion rate of cellulose enzymatic hydrolysis and realizes comprehensive utilization of the lignocellulose biomass resources.

The invention discloses a lignocellulose pre-processing method, which comprises the following steps: pre-impregnating, dehydrating, steam-exploding or extruding and swelling. According to the lignocellulose pre-processing method disclosed by the invention, in a pre-processed product, a hemicellulose recovery rate and a cellulase enzymatic hydrolysis conversion rate are high, and a material processing degree is high; and in addition, the acetic acid concentration of a lignocellulose-containing raw material, which is pre-processed by the method disclosed by the invention, is significantly reduced during fermenting, namely the yield of a fermentation inhibitor drops significantly.

The invention relates to a comprehensive utilization method of lignocellulose biomass. The method comprises the following steps of (a) performing N-stage hydrolysis on the lignocellulose biomass, wherein reaction kettles of the N-stage hydrolysis are connected end to end, one of the reaction kettle is designed as a first stage and a final reaction kettle is designed as an N stage, a freshly prepared acid solution is added in the first stage reaction kettle, a final pentose solution obtained by hydrolysis is discharged from the N stage reaction kettle, final acid hydrolysis residues obtained by acid hydrolysis are discharged from the first stage reaction kettle, and new materials are loaded on the first stage reaction kettle, the first stage hydrolysis reaction time is 1-4 hours, the subsequent hydrolysis reaction time is reduced by 0.25-1.5 hours stage by stage, and N is an integer larger than or equal to 2; (b) performing enzymatic hydrolysis on the acid hydrolysis residues in the step (a) by using cellulose, so that a glucose solution and enzymatic hydrolysis residues are obtained; and (c) treating the enzymatic hydrolysis residues produced in the step (b) with an alkali solution, so that lignin in the enzymatic hydrolysis residues can be extracted. The above method realizes maximized resource utilization of the lignocellulose biomass.

The invention discloses a method for extracting and separating kaempferol from Ligustrum sinense branches and leaves, which comprises the following steps: carrying out thermal-soak extraction on Ligustrum sinense twice, regulating the pH value, enriching with macroporous adsorbent resin, and flushing the columns with deionized water flushing liquid; after the effluent liquid becomes clear, eluting the flushed column filler with 0.8-1 wt% sodium hydroxide eluting liquid, and collecting the eluate; and regulating the pH value of the eluate, carrying out enzymolysis, crystallizing, filtering, and recrystallizing multiple times, thereby obtaining the yellow acicular crystal kaempferol refined product. The method has the advantages of low production cost and wide industrialization prospects, is simple to operate and can easily implement industrial production.

A disclosed method for obtaining high-purity hesperetin from valeriana jatamansi residue comprises: heating valeriana jatamansi residue from which total terpenoid is extracted, leaching for 2 times, adjusting pH of the leaching solution, utilizing macroporous adsorption resin column for gathering hesperetin, firstly eluting with deionzed water, when the eluate is cleared, then eluting with a sodium hydroxide solution with a mass concentration of about 1%, gathering the eluate, adjusting the pH value of the eluate, and performing enzymatic hydrolysis, centrifugation filtering and recrystallization to obtain high-purity hesperetin which is light yellow acicular crystal. The method is simple in operation, low in production cost and high in source utilization rate, and is easy for industrialized production.

The invention relates to the field of bioengineering and the field of chemical engineering, and discloses a pre-treatment method of lignocellulose. The method comprises the step of sequentially performing rinsing impurity removal treatment, extrusion dewatering treatment and steam blasting treatment on lignocellulose raw materials, or sequentially performing rinsing impurity removal treatment, uniform mixing treatment with acid-containing or alkali-containing liquid, extrusion dewatering treatment and steam blasting treatment on the lignocellulose, wherein before the extrusion dewatering treatment, the lignocellulose raw materials are in contact with acid-containing or alkali-containing liquid in at least one treatment process. The method provided by the invention has the advantages that the hemicelluloses recovery and utilization rate and the cellulose enzymolysis conversion rate can be greatly improved; impurities can also be effectively removed; and the operation cost is reduced.

The invention relates to a comprehensive utilization method of lignocellulose biomass. The method comprises the following steps of (a) performing N-stage hydrolysis on the lignocellulose biomass, wherein a reduced range of the concentration of an acid solution from a first-stage to an N-stage stage by stage is 10-40% that of the first-stage acid solution, and N is an integer larger than or equal to 2; (b) performing enzymatic hydrolysis on the acid hydrolysis residues in the step (a) by using cellulose, so that a glucose solution and enzymatic hydrolysis residues are obtained; (c) treating the enzymatic hydrolysis residues produced in the step (b) with an alkali solution, so that lignin in the enzymatic hydrolysis residues can be extracted and alkali hydrolysis residues are obtained; and (d) returning the alkali hydrolysis residues obtained by alkali hydrolysis treatment to the step (b) after the step (c) is finished so as to perform enzymatic hydrolysis, or firstly mixing the alkali hydrolysis residues with fresh acid hydrolysis residues and then carrying out the enzymatic hydrolysis of the step (b), performing the step (c) and the step (d) successively, repeating such process, and further extracting the lignin and performing cellulose enzymatic hydrolysis. The above method realizes maximized resource utilization of the lignocellulose biomass.

The invention provides a method for promoting production of ethanol from poplar through hydrothermal and NaOH aqueous solution pretreatment. The method comprises the following steps: crushing poplar, reacting with water at 180-200 DEG C for 5-30 minutes, mixing filter residues obtained by separation with a NaOH aqueous solution, reacting at 80-120 DEG C for 30-120 minutes, separating to obtain pretreated residues, adding cellulase, a saccharomycetes activating solution and a nutrient solution, and carrying out diastatic fermentation. According to the method, a hydrothermal and NaOH aqueous solution two-section pretreatment combined mode is adopted, the influence of lignin on the enzymolysis and fermentation process is reduced, on the premise that the alkali concentration is reduced, production of ethyl alcohol from poplar can be effectively promoted, and the ethyl alcohol yield reaches up to 50.91%.

The invention relates to a comprehensive utilization method of lignocellulose biomass. The method comprises the following steps of (a) performing acid hydrolysis on the lignocellulose biomass to obtain a pentose solution and acid hydrolysis residues after separation; (b) treating the acid hydrolysis residues with an alkali solution so as to extract alkali lignin; and (c) performing enzymatic hydrolysis on the alkali hydrolysis residues obtained by alkali solution treatment in the step (b) by using cellulose so as to obtain a solution with glucose as a main component, wherein the cellulose is obtained by culturing a strain of penicillium, the penicillium is named as penicillium recumbent PD-G3-08 which is preserved in Wuhan University, China Center for Type Culture Collection, with a preservation number of CCTCC M 2011195. The above method increases conversion rate of cellulose enzymatic hydrolysis and realizes comprehensive utilization of the lignocellulose biomass resources.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing acid hydrolysis of the lignocellulose biomass, separating to obtain a pentose solution and acid hydrolysis residues; (b) performing enzymatic hydrolysis of the acid hydrolysis residues in step (a) by using cellulase to obtain a glucose solution and enzymatic hydrolysis residues, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC NO: M 2011195; (c) processing the enzymatic hydrolysis residues produced in step (b) by using an alkaline solution so as to extract lignin from the enzymatic hydrolysis residues. The method realizes the maximum resource utilization of lignocellulose biomass.

The invention discloses a method for extracting and separating kaempferol from Ligustrum sinense branches and leaves, which comprises the following steps: carrying out thermal-soak extraction on Ligustrum sinense twice, regulating the pH value, enriching with macroporous adsorbent resin, and flushing the columns with deionized water flushing liquid; after the effluent liquid becomes clear, eluting the flushed column filler with 0.8-1 wt% sodium hydroxide eluting liquid, and collecting the eluate; and regulating the pH value of the eluate, carrying out enzymolysis, crystallizing, filtering, and recrystallizing multiple times, thereby obtaining the yellow acicular crystal kaempferol refined product. The method has the advantages of low production cost and wide industrialization prospects, is simple to operate and can easily implement industrial production.

The invention relates to an enzymatic hydrolysis product of total flavonoids of Epimedium, which is produced by enzymatic hydrolysis of total flavonoids of Epimedium through immobilized snail enzymes, and the enzymatic hydrolysis product has higher Better anti-tumor activity and better oral absorption. The invention also discloses a preparation method of the enzymatic hydrolysis product. The method uses immobilized enzymes to enzymolyze the total flavonoids of Epimedium herba, and the selected helicase has high binding efficiency, good stability, and high enzymatic hydrolysis conversion rate with the immobilized carrier. Moreover, the immobilized enzyme can also be recycled, which can save resources and realize green production. The present invention also relates to the application of the enzymatic hydrolyzate in anti-tumor. The total flavonoid enzymatic hydrolyzate of Epimedium herba obtained according to the technical scheme provided by the present invention is effective against human lung cancer A549 cells, liver cancer HepG2 cells, breast cancer MCF‑7 cells, cervical cancer Hela cells have significant proliferation inhibitory effect and can be used for the treatment of lung cancer, liver cancer, breast cancer and cervical cancer.

A method for preparing lignocellulose from biomass based on low-intensity steam explosion is disclosed. The method includes 1) biomass raw material treatment, 2) low-intensity steam explosion pretreatment, 3) extraction to separate hemicellulose, and 5) cellulase enzymolysis and saccharification. Carbon in the wooden biomass is converted into useful products to the utmost, the cellulose enzymolysis conversion rate is further improved, and the conversion cost is reduced so that lignocellulose resources are fully utilized. A treatment process does not use acid, alkali or products containing sulfur and chlorine, and is a green pollution-free separation process. The method has low requirements on equipment. The hemicellulose and lignin are separated by adopting a pressurizing rapid solvent extraction manner, thus greatly reducing the cost of extraction separation. Only water and ethanol which are easy to recover are adopted in the method, thus achieving zero emission, and the method is ofpositive significance for environment protection.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing alkaline hydrolysis of the lignocellulose biomass to extract lignin; (b) performing acid hydrolysis of the residues obtained in the alkaline hydrolysis processing , separating to obtain a pentose solution and acid hydrolysis residues; (c) performing enzymatic hydrolysis of the residues obtained by alkaline hydrolysis processing in step (b) by using cellulase to obtain a solution with a main component of glucose, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M 2011195 and a preservation date of June 13, 2011. The method improves the extraction ratio of cellulose enzymatic hydrolysis, and realizes comprehensive utilization of lignocellulose biomass resources.

The invention relates to a comprehensive utilization method of lignocellulose biomass. The method comprises the following steps of (a) performing acid hydrolysis on the lignocellulose biomass to obtain a pentose solution and acid hydrolysis residues after separation; (b) treating the acid hydrolysis residues with an alkali solution so as to extract alkali lignin; (c) performing enzymatic hydrolysis on the alkali hydrolysis residues obtained by alkali solution treatment in the step (b) by using cellulose so as to obtain a glucose solution and enzymatic hydrolysis residues, wherein the cellulose is obtained by culturing a strain of penicillium, the penicillium is named as penicillium recumbent PD-G3-08; and (d) returning the enzymatic hydrolysis residues obtained by alkali solution treatment to the step (b) so as to perform alkali solution treatment, or firstly the enzymatic hydrolysis residues with fresh acid hydrolysis residues and then carrying out the alkali solution treatment of the step (b), performing the step (c) and the step (d) successively, repeating such process, and further extracting the lignin and performing cellulose enzymatic hydrolysis. The above method increases conversion rate of cellulose enzymatic hydrolysis and realizes comprehensive utilization of the lignocellulose biomass resources.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing alkaline hydrolysis of the lignocellulose biomass to extract lignin; (b) performing acid hydrolysis of the residues obtained in the alkaline hydrolysis processing , separating to obtain a pentose solution and acid hydrolysis residues; (c) performing enzymatic hydrolysis of the residues obtained by alkaline hydrolysis processing in step (b) by using cellulase to obtain a solution with a main component of glucose, wherein the cellulase is obtained by culturing a strain of penicillium which has a classification name of Penicillium decumbens PD-G3-08, and is preserved in Wuhan University China Center for Type Culture Collection with a preservation number of CCTCC M 2011195 and a preservation date of June 13, 2011. The method improves the extraction ratio of cellulose enzymatic hydrolysis, and realizes comprehensive utilization of lignocellulose biomass resources.

A comprehensive utilization method of lignocellulose biomass comprises the following steps: (a) performing N-stage acid hydrolysis of lignocellulose biomass, wherein the concentrations of the acid solutions decrease gradually from the first stage to the Nth stage by 10-40% of the concentration of the first stage acid solution,, and N is an integer more than or equal to 2; (b) processing the acid hydrolysis residues by using an alkaline solution so as to extract alkaline lignin; (c) performing enzymatic hydrolysis of the alkaline hydrolysis residues obtained by alkaline solution processing in step (b) by using cellulase to obtain a glucose solution and enzymatic hydrolysis residues; (d) after step (c) is complete, returning the obtained enzymatic hydrolysis residues to step (b) for alkaline solution processing, or combining the enzymatic hydrolysis residues with new acid hydrolysis residues and performing alkaline solution processing in step (b), then orderly performing steps (c) and (d), cycling the steps so as to further extract lignin and to perform enzymatic hydrolysis of the celluose. The method realizes the maximum resource utilization of lignocellulose biomass.

The invention discloses a method for preprocessing lignocellulose, which comprises: installing the lignocellulose into a recycle reactor, injecting diluted acid, opening a circulating pump to carry out cyclic reaction under the temperature of 50DEG C-200DEG C, acid-removing and filtering hydrolyzate which is obtained after finishing the reaction, and using the hydrolyzate to ferment. The inventionalso discloses a system which is applied in the method. The method of the invention can effectively increase the conversion rate of hemicellulose and the conversion rate of cellulase hydrolysis in the lignocellulose, fermentation inhibiting products are reduced, simultaneously, the time for preprocessing also can be shortened, and the energy consumption is lowered.