The invention relates to a pulsation combustion type ultrasonic-assisted reinforced heat transfer boiler. A pulsation combustion system comprises a gas mixing chamber and a pulsation combustor, the gas mixing chamber is connected with an air inlet pipe and a natural gas inlet pipe correspondingly, a plurality of straight pipe heat exchange pipes, a liquid inlet, a liquid outlet, a mixed gas inlet,a mixed gas outlet, a liquid inlet pipe plate and a condensed water drain outlet are arranged on a heat exchanger, the liquid inlet is connected with the liquid inlet pipe plate, the plurality of straight pipe heat exchange pipes are connected between the liquid inlet pipe plate and the liquid outlet in parallel, a flame nozzle of the pulsation combustor is connected to the mixed gas inlet, the mixed gas outlet is connected with a chimney, and the ultrasonic-assisted reinforced heat transfer system comprises an ultrasonic transducer and a pressure regulator, and the ultrasonic transducer is connected to the pressure regulator. The system is scientific and reasonable in structural design, the problems of scaling of the boiler, pipe explosion of a water-cooling wall pipe and an arch pipe and overheating bulging of the pot barrel lower part can be solved, meanwhile, the combustion efficiency of the natural gas can be improved, the energy is saved, and the heat exchange efficiency is improved.