63results about How to "Increase cell concentration" patented technology

Novel methods and apparatus are disclosed for cell culture and cell recovery. The methods and apparatus simplify the process of cell separation from media, minimize potential damage to gas permeable devices during fluid handling, and allow closed system automated cell culture and cell recovery from gas permeable devices.

A method for repairing tissue of a selected organ from among heart, brain, liver, pancreas, kidney, glands, and muscles in a patient's body. Adult stem cells that have the capability to repair tissue of the selected organ are recovered by harvesting from the patient's body. The harvested stem cells are then intraluminally applied through a designated natural body vessel. During the time the stem cells are being applied to the targeted tissue downstream, the designated vessel or duct is selectively occluded to increase concentration and pressure of the applied adult stem cells by the vessel.

The invention provides a method for producing biogas through high-solid two-phase three-stage anaerobic digestion by using perishable organic wastes, which comprises the following steps: the perishable organic wastes are hydrolyzed in a hydrolysis acidogenic reactor, and a mixture obtained after hydrolysis is acidified to generate a large number of organic acid products with low molecular weight; the bottom of the hydrolysis acidogenic reactor is communicated with the bottom of a methanogenic reactor through a circulation pump and a pipeline; the top of the methanogenic reactor is communicated with the top of the hydrolysis acidogenic reactor; and the acetic acid producing reaction is carried out in the methanogenic reactor and the methane producing reaction is continuously carried out. The invention can separate the hydrolysis acidogenic process from the acetic acid and methane producing process in the anaerobic digestion process so as to prevent organic acids generated by the perishable organic wastes from inhibiting methanogenesis. The device has simple processing procedure, low cost, easy maintenance and stable and reliable performance, is suitable for anaerobic digestion treatment of various perishable organic wastes, and has highly efficient and stable biogas production capacity of the perishable organic wastes through the anaerobic digestion.

The invention discloses a cultivating and producing method for haematococcus pluvialis. A haematococcus pluvialis green swarm cell is inoculated to a culture medium in a sealed tank body, a manual light-emitting diode (LED) light source is utilized for irradiation cultivating, the wavelength range of a monochromatic light emitted by the LED light source is 450nm to 640nm with the bandwidth of 30nm, two types of light sources with different wavelengths are simultaneously used according to the proportion of light intensity of red lights and blue lights, which is in a range from 2:1 to 5:1, the total light intensity is in a range from 80mu E / m2.s to 1500mu E / m2.s, the temperature during the cultivating process is maintained in a range from 15 DEG C to 28 DEG C, and the potential of hydrogen (pH) of the cultivating environment is controlled in a range from 8.0+ / -1.0 by adding carbon dioxide. The energy consumption of temperature controlling is remarkably saved, the cultivating period is shortened by above 50% compared with that of traditional methods, and the concentration of the obtained haematococcus pluvialis cell in a green algae liquid is 2 to 5 times of that of the haematococcus pluvialis cell in a green algae liquid by traditional methods.

The invention provides a device for producing biogas by two-phase three-section anaerobic digestion of high solids of perishable organic wastes. The device comprises a hydrolysis acid-production reactor and a methane-production reactor, wherein the two reactors are connected with each other, and the bottoms of the reactors are communicated through a circulating pump and a pipe; the hydrolysis acid-production reactor is a solid infiltration bed reactor, the top is provided with a water distributor and a spray header, the middle-upper part is provided with a feeding port, the middle-lower part is provided with a perforated plate and a discharging port, the bottom is provided with a residue-discharging port, and infiltration fillers are arranged on the upper surface of the perforated plate; and the methane-production reactor consists of a filter filler bed and a fiber filler bed, wherein the filter filler bed is arranged on the lower part of the methane-production reactor, the filter filler bed is arranged on the upper part of the methane-production reactor, filter fillers are arranged in the filter bed, a fiber filler frame is arranged in the fiber filler bed, and the fiber filler frame is wound by fiber fillers. The device has the advantages of simple processing procedures, low cost, easy maintenance, stable and reliable performance and suitability for the anaerobic digestion treatment of various perishable organic wastes, and has the capability of producing the biogas in high efficiency and stably by the anaerobic digestion of perishable organic wastes.

The invention relates to a method for preparing fecal degradation bacterial agent, which comprises the following steps: inoculating fecal degradation bacteria on the beef extract peptone culture medium culture medium bevel; culturing under the temperature of 32-38 DEG C for 18-24 h to obtain the bevel, inoculating the bevel seed in the liquid seed culture medium, oscillating, aerating and culturing under the temperature of 32-38 DEG C and the rotary speed at 120-200 r / min for 18-24 h to obtain the liquid seed, inoculating the liquid seed in the solid culture medium whose water content is 50-70% under the inoculation quantity of 1-10%, blending evenly, aerating and culturing statically under the temperature at 32-37 DEG C for 48-72 h, finishing culturing when the cell density reaches to 10 <6>-10 <9> g, obtaining the solid culture, drying the solid culture or drying under the temperature at 20-60 DEG C, grinding the dried solid culture into the fecal degradation bacterial agent. The bacterial agent of the invention is very simple in preparation, low in cost, long in preservation and convenient in use.

The invention discloses a nutrient solution for arranged flowers. The nutrient solution comprises the raw materials in percentage by weight: 0.8-1.2% of saccharose, 0.7-1.4% of glucose, 0.08-0.105% of edible salt, 0.025-0.035% of aspirin, 0.1-0.14% of vitamin C, and the balance of water. Compared with the prior art, the nutrient solution has the following advantages that nutrient components required by arranged flowers are supplied to guarantee normal metabolism of the arranged flowers; the breath speed of the arranged flowers is slowed down, and the aging and withering of the arranged flowers is reduced; and because the activity of microorganism is blocked, the phenomenon of untimely rotting and decaying of the arranged flowers is avoided. By using the nutrient solution for the arranged flowers, the freshness retaining time of the arranged flowers can keep for 20-30 days, and the color is bright and the luster is lovely.

A method is described for repairing tissue of a selected organ from among heart, brain, liver, pancreas, kidney, glands, and muscles in a patient's body. In the method, adult stem cells that have the capability to repair tissue of the selected organ are recovered by harvesting from the patient's body. The harvested stem cells are then intraluminally applied through a designated natural body vessel or duct leading to a predetermined target site of the tissue of the selected organ to be repaired. During the time the stem cells are being applied to the targeted tissue downstream, the designated vessel or duct is selectively occluded to increase concentration and pressure of the applied adult stem cells at the target site of the organ tissue.

The invention discloses a method for leaching chalcopyrite through a reinforced iron oxidized culture and belongs to the technical field of bioleaching. The method includes the steps that on the basis of a 9K-chalcopyrite complex medium, a ferrous energy substrate is added through an impulse type supplementary material in the middle and later periods of cultivation, and ferric oxide thiobacillus is cultured at high density; centrifugal operation and suspended elution cells of the 9K medium are adopted for removing jarosite, the cells not containing jarosite are adopted for inoculation, the inoculation size is increased properly, and meanwhile ferrous ions are supplemented and included to shorten a lag phase; and the pH of lixivium is lowered level by level in the later leaching period, accumulation of the jarosite is reduced, and the leaching effect of the chalcopyrite is improved in the whole process. By the adoption of the method, the iron oxidized culture can be more efficiently cultured, the lag phase can be shortened, the passivation effect caused by accumulation of the jarosite can be weakened, the leaching process of the chalcopyrite is improved while iron metabolism is reinforced, operation is easy and feasible, and the method is suitable for being applied and popularized on a large scale.

The invention discloses a preparation method of high-activity lactobacillus paracasei N1115 ready-to-eat probiotic powder. The preparation method comprises four steps of sequentially performing strainactivation, performing proliferation culturing, collecting thalli, performing freeze drying and performing milling to obtain powder. The high-activity lactobacillus paracasei N1115 ready-to-eat probiotic powder provided by the invention can also maintain the state that the viable count is not less than 108CFU / g at the later stage of the quality guarantee period of 18 months, and the mouth feel isgood. The preparation method is used for preparing the ready-to-eat probiotic powder, and after the ready-to-eat probiotic powder is taken, the blood sugar can be reduced.

The invention relates to the microorganism agent for degrading hogwash garbage and the preparation craft, which is formed by mixing seven active germ powders in certain matching. The seven germs are as following: Bacillus subtilis E4, H11, Bacillus licheniformis A2, I1, Bacillus cereus F3, F6 and Bacillus stearothermophilus H13. The invention resolves the problems of bad degrading result and complicated preparation of the hametz due to overfull germ sorts existing in the import microorganism agent degrading the oil component in the garbage, and adopts bacillocin as the strain to obtain the germ powder through the crafts of seed culturing, fermentation culturing and sponging drying. The invention has the advantages of small sorts of germ, simple fermenting process, high concentration of germ in the germ powder and good degrading result, and can achieve minimization treatment, innocent treatment and treatment of being turned into resources, therefore can not only improve the environment sanitary of the city, but also lay a solid foundation for large-scale commercial process. The invention also has an advantage of good economic benefit.

An arrangement for taking a sample of cells from a suspicious lesion or a tumour with the so called fine needle aspiration technique, provides a good penetration of tumours, especially small and / or hard fibrous tumours, and in the meantime yielding an increased amount of cells in the sample, by applying a longitudinal movement to the needle when the needle is penetrating the tumour and by applying both a rotational and a longitudinal movement to the needle when the needle is positioned inside the tumour. The arrangement is further provided with heat generating elements in order to apply a short pulse of heat to the needle in order to lower the risk for the tumour to spread.

The invention discloses a bioreactor for high-density large-scale animal cell culture and application thereof, and belongs to the field of biological cell culture. According to the bioreactor for high-density large-scale animal cell culture, sufficient oxygen is provided in a low shear environment to meet the need of cell growth by using multi-stage microporous aeration combined with slight axial agitation or circulation, and also attenuate the dissolved oxygen gradient within the reactor. At the same time, a wire mesh demister is mounted on the top of the reactor, and external circulation spray is combined to eliminate physical damage of the cells caused by bubble breakage. The bioreactor for high-density large-scale animal cell culture has the following beneficial effects: a demisting device is arranged on the liquid layer of the reactor so that bubbles do not break on the surface of the liquid; and in addition, a gas permeable membrane is used to selectively remove dissolved carbon dioxide in the reactor to eliminate inhibition of the carbon dioxide on cell growth.

The invention relates to a growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and a using method of the growth promoting agent. The growth prompting agent is obtained as follows: adding 1 part weight of one or a mixture of sorbic acid, potassium sorbate or sodium sorbate to 1-4 parts by weight of alcohol for stirring, mixing and dissolving. The using method of the growth promoting agent is as follows: adding the growth promoting agent to a prepared photosynthetic bacteria liquid medium for stirring until the growth promoting agent is sufficiently dissolved; adding active photosynthetic bacteria strains for filling to a sealed or semi-open light transmission container for culturing at 25-32 DEG C by radiating under 1500lux-1600lux, wherein sorbate radial concentration of the growth promoting agent, which is added to the photosynthetic bacteria liquid medium, is controlled within a range of 0.005 mmol / L-1.0 mmol / L. The growth promoting agent for promoting photosynthetic bacteria strain to quickly breed is simple to use, less in usage, low in cost and capable of promoting photosynthetic bacteria strain to quickly breed. Moreover, the using method of the growth promoting agent is safe, ecological and environment-friendly and extensive in market prospect.

The invention provides a method for fermenting and producing coenzyme Q10, comprising the following steps: firstly carrying out high-density aerobic fermentation for 60 to 84 hours on agrobacterium tumefaciens by inoculum size of 5 to 15 percent; then carrying out separation on the obtained fermentation liquor and obtaining separated thallus; and finally suspending the separated thallus, then backflowing, extracting and obtaining the coenzyme Q10, wherein the fermentation culture medium comprises the following components by weight percent: 12 to 20 percent of glucose, 0.5 to 1.5 percent of ammonium sulfate, 0.1 to 0.5 percent of monopotassium phosphate, 0.01 to 0.05 percent of magnesium sulfate, 0.5 to 2.5 percent of corn steep liquor and 0.2 to 1 percent of diammonium hydrogen phosphate; and pH is 6 to 7.5 and the fermentation temperature is 28 to 31 DEG C. The method has simple technique, low thallus concentration of the fermentation liquor and low production cost and is applicable to large-scale production.

A method for treating wastewater containing organic contaminants is disclosed. Wastewater containing organic contaminants is fed into an outer pipe of a pipe-in-pipe assembly, wherein the outer pipe concentrically surrounds an inner pipe. Oxygen is fed into the inner pipe which is rotatably mounted and is provided with openings, thereby to provide different sizes of oxygen bubbles to the outer pipe. The oxygen is dispersed into an annular portion between the outer pipe and the inner pipe thereby contacting the wastewater with oxygen; and the thus treated wastewater is collected. The inner pipemay be a tunable membrane material, and the outer pipe may have a biocatalyst material present on its inner surface.

The present invention provides one method of culturing photosynthetic bacteria with ultrahigh cell density. The present invention features that in the photosynthetic bacteria culturing medium, ethanol is used as the unique organic carbon source and the ethanol concentration in the liquid culture medium is controlled in 1.0-10.0 g / L. The present invention has the advantage of making photosynthetic bacteria grow continuously to reach high cell density while remitting the suppression of raised pH value on the growth of photosynthetic bacteria.

The invention discloses a Chlorella zofingiensis heterotrophic high-density culture method which is characterized in that Chlorella zofingiensis is inoculated into a fermentation tank and cultured, aused basal culture medium and a used fed-batch culture medium take ammonium salt as a nitrogen source, the carbon nitrogen ratio of the basal culture medium is 5:(1-80):1, the carbon nitrogen ratio ofthe fed-batch culture medium is 5-20 times that of the basal culture medium, the pH (potential of hydrogen) value of a culture system is monitored in real time during culture, adjusted by adding ammonia water according to monitoring results and kept 6.5+ / -0.2 to supplement the nitrogen source, the glucose concentration of the culture system is monitored in real time during culture, feeding of thefed-batch culture medium is started when the glucose concentration of the basal culture medium is reduced to 3-4 g / L, and the variation amplitude of the glucose concentration of the culture system islower than 20% within any two hours by the aid of feeding speed. According to the characteristic of pH reduction in the ammonium salt using process of cells, the pH is regulated by the aid of the ammonia water to supplement the nitrogen source. The culture method can effectively avoid the inhibitory effect of nitrogen source excess or deficiency in an existing fixed carbon nitrogen ratio feedingculture mode on cell growth.

The invention discloses a complex method for improving efficiency of chalcopyrite leaching conducted through sulfur oxidation cultures and belongs to the technical field of biology. The method comprises the steps that on the basis of a Starkey-chalcopyrite composite culture medium, an external-source energy substrate, namely elemental sulfur, is supplemented in a pulsed manner at the middle and later culture stages, and high-density culture of thiobacillus thiooxidans is achieved; a proper amount of elemental sulfur and iron ions are added while cell inoculation is conducted, start of a leaching biochemical reaction cycle is accelerated, the lag phase is shortened, and the inoculum size is increased; and at the later leaching stage, the low constant pH is maintained, jarosite stress is inhibited, sulfur oxidation culture cells are added as supplementation, iron metabolism is promoted while sulfur metabolism is improved, and furthermore, the leaching microenvironment is improved. By means of the method, the sulfur oxidation cultures can be cultured more efficiently, the leaching lag phase is shortened, and active biochemical reaction state of the leaching microenvironment is maintained better, and thus the leaching rate is increased; and in addition, the method is easy to operate and implement and suitable for being applied and popularized in similar bioleaching processes on a large scale.

The invention relates to a culture method of microbe algae and in particular relates to an industrialized culture method for high-protein desert algae. The method comprises the following steps: 1, separating a desert alga culture; 2, inoculating a single desert alga colony to a fixed plate, and repeatedly culturing until eight or ten generations are inoculated to obtain the algae; 3, inoculating the algae into a liquid culture medium, and performing industrialized enclosed culture for 3-15 days. The desert algae are cultured at first, the cell concentration in the desert soil culture solution is improved, a target alga desert soil culture solution with high target alga biomass is obtained, the target algae are separated and purified to obtain high-yield target algae, and finally large-scale industrialized culture is performed, so that the target algae can be successfully separated. Moreover, the adopted culture method is totally sterile culture, pollution caused by pollution sources and harmful bacteria is avoided, and the desert algae can be cultured in a large scale under the condition that the yield of the desert algae is guaranteed.

Fine needle arrangement for taking a sample of cells from suspicious lesions by using fine needle aspiration (FNA) technique, including a tubular needle member (1), a storage compartment (2) enclosing a chamber, and a coupling (3) to which a connector may be attached. The chamber is configured with a gradually increasing cross-sectional area from the distal part of the chamber to the proximal part of the chamber, such that the chamber has no residual spaces where cell samples may be trapped, and that an efficient air streaming is achieved.

The invention discloses a method for improving efficiency of leaching chalcopyrite from thiobacillus and belongs to the technical field of bioengineering. According to the method disclosed by the invention, on the basis of an improved Starkey-chalcopyrite composite culture medium, iron ions and ferrous ions are supplemented at the initial stage of culture and elemental sulfur is added at stages inthe bio-leaching process, various biochemical reactions in the leaching environment are regulated, chemical and biological factors in the bio-leaching process are enhanced, iron metabolism is promoted while sulfur metabolism is improved, and production of sulfur passivation films and iron passivation films is reduced, so that the leaching rate is improved. The method is simple and feasible in operation, and is applicable to large-scale popularization and application of similar bio-leaching processes.

The invention provides a method for culturing Clostridium butyricum T4 with ultra-high cell concentration. The method is characterized by comprising the following steps of: promoting the Clostridium butyricum T4 to grow in the conventional Clostridium butyricum T4 culture medium by using urea as physiologically alkaline salt; and controlling the concentration of the urea in the liquid culture medium to be within the range of between 1.0 and 3.0g / L. The method has the advantages that: the inhibition effect produced on the growth of the Clostridium butyricum T4 due to the overquick reduction of the pH value of the culture medium can be relieved to a certain degree; meanwhile, the sustained growth of the Clostridium butyricum T4 can also be promoted so as to finally achieve the higher cell concentration. The industrialized production of a Clostridium butyricum T4 liquid with ultra-high cell concentration is promoted.

The invention discloses a biologic fresh-keeping microbial inoculum fermented with yellow serofluid generated during making bean curds and an application of the biologic fresh-keeping microbial inoculum to fresh keeping of fruits and vegetables. According to the biologic fresh-keeping microbial inoculum, fermentation liquid obtained by fermenting the yellow serofluid which is generated during making bean curds and is used as the fermentation raw material with bacillus subtilis is directly used as fresh-keeping microbial inoculum, wherein the thalli concentration in the fermentation liquid achieves 1*10<13>-2.7*10<13>CFU / ml, the gamma-polyglutamic acid concentration achieves 30-50g / L, and the antibacterial peptide yield achieves 1.2-2g / l. Compared with obtaining methods of other biologic fresh-keeping microbial inoculum, the obtaining method of the biologic fresh-keeping microbial inoculum is lower in cost and more environmental-friendly, waste resources namely the yellow serofluid generated during making bean curds can be used, so that the potential safety hazards that during natural fermentation of the yellow serofluid during processing of the bean products, sour pulp can generatepathogenic bacteria can be reduced, and besides, the gamma-polyglutamic acid content and the antibacterial material content in fermented products are high; and therefore, the biologic fresh-keeping microbial inoculum has favorable fresh-keeping effects on picked fruits and vegetables, is free from pollution and free from nuisance, and has long development prospects and industrial utilization rate.

The invention discloses a carrier attachment type cyclic culture device of a heterotrophic nitrification-aerobic denitrification bacterial agent. The carrier attachment type cyclic culture device comprises a main reactor and a pre-reactor, wherein a material supplement port and a culture medium injection port are formed at the top of each reactor, an aeration device, a sedimentation area and a discharge valve are arranged at the lower part of each reactor, the reactors are respectively connected with two solid-liquid separators through the discharge valves; and the main reactor is communicated with the solid-liquid separator of the pre-reactor through the material supplement port. The pre-reactor utilizes a residual solution after culture of the main reactor for re-culture and an unsaturated carrier is further transferred into the main reactor for further culture. The carrier attachment type cyclic culture device disclosed by the invention can fully utilize a culture medium solution, provide a pre-attachment process for the carrier, improve the utilization rate of a culture medium and the attachment efficiency of the carrier, avoid the excessive loss of the bacterial agent, improve the stability and the tolerance of the bacterial agent and realize the self-control continuous operation of the two reactors.

The invention discloses a cyclic beta-1,2-glucan fermenting method capable of inhibiting chromatogenesis and belongs to the field of intelligent light industry process control. The cyclic beta-1,2-glucan fermenting method disclosed by the invention successfully inhibits chromatogenesis in a Rhizobium radiobacter NBRC 13259 fermenting process, improves a cell concentration to 8.0g / L, solves the problems of difficulty in controlling chromatogenesis and difficulty in improving the cell concentration in the Rhizobium radiobacter NBRC 13259 fermenting process and establishes foundation for improving a cyclic beta-1,2-glucan concentration in a unit volume.

The invention discloses a cosmetic peptide composition modified by a targeting peptide. The cosmetic peptide composition is a covalent / non-covalent compound composed of a targeting peptide and a cosmetic peptide. The targeting peptide is a linear or annular peptide composed of 3-30 amino acids, and comprises RGD polypeptide of targeting cell surface integrin alpha v beta 3, Otx2 polypeptide of targeting cell surface chondroitin sulfate or cell-penetrating peptide in targeting cell. The cosmetic peptide comprises acetylated, fatty acid acylated or non-acylated active peptides which are common in the market and exert effects inside and outside cells, and the targeted cosmetic peptide composition does not affect the activity of the cosmetic peptide, is used for skin cosmetic treatment, promotes cell surface aggregation or intracellular guidance of the cosmetic peptide, and improves the skin whitening effect. According to the cosmetic peptide, the combination opportunity of the cosmetic peptide and intracellular and extracellular receptors is improved, the use effect of the cosmetic peptide is enhanced, especially the effects of resisting oxidation and aging, increasing the collagen content of skin tissue and repairing the keratinous structure of skin cells are achieved, and the cosmetic peptide has good application potential in the field of cosmetic raw materials.

The invention discloses a carrier attached cyclic culture method of heterotrophic nitrification-aerobic denitrifying bacteria, wherein a light spherical filler having the diameter of 3cm is taken as a carrier, beer wastewater and various kinds of industrial wastewater are taken as a culture base fluid, and a culture device is composed of a main reactor and a pre-reactor. The pre-reactor is used for subculture by using the residual liquid left after the culture in the main reactor is finished; the unsaturated carrier is then transferred to the main reactor for further culture; the culture base liquid is utilized sufficiently and a pre-attachment process is provided for the carrier; therefore, the utilization rate of the culture medium and the attachment efficiency of the carrier are improved, and the self-control continuous operation of the two reactors is realized. The carrier attached cyclic culture method avoids too much loss of the bacteria; the bacterial can be saved more conveniently after being air-dried, high cell concentration is obtained, and the stability and endurance capacity of the bacteria are improved.

The invention discloses a method for improving soluble expression quantity of beta-cyclodextrin glucosyltransferase, and belongs to the technical field of enzyme engineering and fermentation engineering. In order to improve the yield of beta-cyclodextrin glucosyltransferase, the invention provides a strategy for improving the soluble expression of foreign protein in recombinant escherichia coli by changing the dissolved oxygen level of a fermentation culture medium and adding metal ions. An effective strategy is provided for efficient expression of the beta-cyclodextrin glucosyltransferase, the operation process is simple, the cost is low, the effect is remarkable, a certain foundation is laid for industrial production of the beta-cyclodextrin glucosyltransferase, and potential industrial application value is achieved.

The invention belongs to the technical field of antifreezing agents for fruit trees and specifically relates to an environment-friendly antifreezing agent for the fruit trees and a preparation methodof the antifreezing agent. The invention provides the environment-friendly antifreezing agent for the fruit trees and the preparation method thereof, aiming to overcome defects in the prior art. According to the preparation method, algal polysaccharides and plant-sourced small-molecule polypeptide are prepared into a nano-like lipidosome solution, prohexadione calcium is prepared into nano microcapsule dispersion liquid, and he environment-friendly anti-freezing agent for the fruit trees is formed through the nano-like lipidosome solution, the nano microcapsule dispersion liquid, glycerinum, vitamin E, sodium alginate, 50 wt% aqueous polyethyleneimine (PEI) solution and mannitol. The prepared antifreezing agent can achieve quick-acting and long-acting antifreezing effects, is applicable tomultiple economic crops, such as apple trees, tea, mulberries, strawberries, grapes and various flower and fruit seedlings, and has the antifreezing effect especially for flower buds, sprouts and leaves of the apple trees and the like.