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36results about How to "Improve the effect of electrophoresis" patented technology

Copolymers for capillary gel electrophoresis

InactiveUS6926815B2Facilitates high level of separation performanceEnsure solubilitySludge treatmentVolume/mass flow measurementCapillary electrophoresisElectrophoresis
This invention relates to an electrophoresis separation medium having a gel matrix of at least one random, linear copolymer comprising a primary comonomer and at least one secondary comonomer, wherein the comonomers are randomly distributed along the copolymer chain. The primary comonomer is an acrylamide or an acrylamide derivative that provides the primary physical, chemical, and sieving properties of the gel matrix. The at least one secondary comonomer imparts an inherent physical, chemical, or sieving property to the copolymer chain. The primary and secondary comonomers are present in a ratio sufficient to induce desired properties that optimize electrophoresis performance. The invention also relates to a method of separating a mixture of biological molecules using this gel matrix, a method of preparing the novel electrophoresis separation medium, and a capillary tube filled with the electrophoresis separation medium.
Owner:APPL BIOSYSTEMS INC

Preparation method of enamel coating

The invention discloses a preparation method of an enamel coating. The method comprises the following steps that 1, a metal matrix is pretreated, wherein the metal matrix is subject to heat preservation for 20-40 min at the temperature ranging from 950 DEG C to 980 DEG C and then decarburized, the decarburized metal matrix is placed into an acid solution for acid pickling and taken out till a clean metal surface is obtained, clean water is used for repeated washing to remove surface residual acid liquor, and finally the metal matrix is placed at the temperature ranging 200 DEG C to 300 DEG C, rapidly dried and placed to a dry pollution-free place for standby application; 2, ceramic glaze powder is prepared; 3, ceramic slurry is prepared, and 4, enamelling and firing are carried out. According to the preparation method, the production process is simplified, the production efficiency is improved, an electrophoresis method is adopted for forming a uniform low-porosity porcelain enamel premade coating, the coating is uniform and compact in texture, oxide particles are small, grains are refined, the macro surface of the coating is flat, smooth and glossy, and cracking and block disengagement phenomena are effectively reduced.
Owner:GUIZHOU XINGREN COUNTY RONGKAI HARDWARE ENAMEL PROD

Process for coloring aluminum sections with pure black pigment

The invention discloses a process for coloring aluminum sections with pure black pigment. The process is mainly used in coloring of aluminum alloy sections after anodic oxidation. A coloring producing process is adjusted, nickel salt deposition in oxidation film holes is changed, then the pure black pigment for coloring of aluminum section after anodic oxidation is produced through a electrolytic coloring single nickel salt coloring process, and a tradition that the anodic oxidation pure black pigment cannot be produced through the electrolytic coloring single nickel salt coloring process is broken.
Owner:肇庆新亚铝铝业有限公司

Semiconductor wafer electroplating clamping device

The invention discloses a semiconductor wafer electroplating clamping device. The device comprises a worktable, a hydraulic rod, a cylinder, a clamping main plate and an electroplating disc; the worktable is of an L-shaped structure, a transverse through bottom groove with a rectangular structure is formed in the bottom end surface of the inner side, a hydraulic rod is mounted on the side, far from the vertical end, of the top end surface of the outer side, and a support top plate is arranged on the top end surface of the inner side; the bottom end of a piston rod of the hydraulic rod penetrates through the worktable, and is fixedly welded with the support top plate; a motor vertically arranged is welded at the bottom end of the support top plate; and a machine shaft of the motor is connected with stirring blades through a support base plate. The semiconductor wafer electroplating clamping device can stir electroplating liquid in an electroplating tank, can guarantee full stirring andflowing of the electroplating liquid to guarantee the electroplating deposition thickness uniformity and to improve the electroplating effect, and finishes the clamping through cylinder pushing to achieve a better and more stable clamping effect.
Owner:扬州国润半导体科技有限公司

Method for producing black aluminum profile by using anodized single nickel salt for coloring and aluminum profile

The invention provides a method for producing a black aluminum profile by using anodized single nickel salt for coloring. The method specifically comprises the steps of aluminum profile surface pre-treatment, aluminum profile surface anodizing, alternate-current electrolytic coloring, boiling water sealing and the like, and in the process of alternate-current electrolytic coloring, the aluminum profile is subjected to voltage control coloring at three stages. Meanwhile, the invention provides the black aluminum profile obtained according to the method. The method for producing the black aluminum profile by using the anodized single nickel salt for coloring is implemented under a normal-temperature environment, and energy consumption is low; a dense anode oxide film with high surface smoothness is obtained after anodizing is completed; and the continuous and stable pure black anodized aluminum profile is produced after three-stage coloring treatment is carried out, and is uniform and bright in surface, small in color difference, good in decoration effect, and well resistant to rust, salt water, salt spray, humidity, heat, cold and scratch.
Owner:肇庆亚洲铝厂有限公司

Hanging tool for electrophoresis

The invention relates to a hanging tool for electrophoresis. The hanging tool comprises a workpiece (5), an end cap (50), an upper piece frame (1) and a support (2), wherein the workpiece (5) is cylindrical; one end of the workpiece (5) is closed and the other end of the workpiece (5) is open; the open end of the workpiece (5) is sealed by virtue of the end cap (50); the workpiece (5) is clamped between the upper piece frame (1) and the support (2); the closed end of the workpiece piece (5) pushes against the upper piece frame (1); and the end cap at the open end of the workpiece piece (5) pushes against the support (2). The hanging tool for electrophoresis is suspended on a conveying machine of an electrophoresis production line and can be used for performing electrophoresis on the cylindrical workpiece; and the workpiece is fixed without moving during electrophoresis, and an electrophoresis solution cannot flow into the workpiece, so that the electrophoresis effect at the outer part of the workpiece is very good.
Owner:江苏舜天新盈轻工业有限公司

Production process of biodegradable electrostatic flocked paper product

The invention relates to the technical field of electrostatic flocked products, and concretely relates to a production process of a biodegradable electrostatic flocked paper product. The production process comprises the following steps: S1, cutting; S2, dyeing; S3, electro-deposition treatment; S4, drying; S5, screening; S6, gluing; S7, flocking; and S8, drying. Added substances comprise, by mass,50 parts of short fibers, 180-200 parts of water, 10-15 parts of a reactive dye, 3-5 parts of a substituted alkali, 1-2 parts of magnesium sulfate, 0.5-1 part of silica sol, 0.4-0.8 part of aluminumacetate, 1-2 parts of a softening agent and 0.3-0.5 part of water glass; and an adhesive comprises, by mass, 80-85 parts of polylactic acid, 1-3 parts of sodium carboxymethyl cellulose and 0.5-1 partof chitosan. The electrostatic flocked paper has the effect of facilitating good biodegradation of the electrostatic flocked paper product.
Owner:广东顺德贰发毛绒有限公司

Lifting device for electrophoresis production line

The invention provides a lifting device for an electrophoresis production line. The lifting device comprises L-shaped conveyor lines, ribbed slabs, tracks, a conveyor car, an electric cylinder, a lifting frame, an I-shaped turning block A, a connecting band and an I-shaped turning block B. The ribbed slabs are also connected between the outer side of the L-shaped conveyor line and the bottom end face of the top plate. The conveyor car is slidingly installed on the tracks of the two L-shaped conveyor lines through conveyor wheels at two ends of the conveyor car. The electric cylinder penetratesthrough a spindle sleeve B in the middle of the conveyor car to be welded with the lifting frame. The top of the electric cylinder is also provided with a spacing board. A rotating shaft of a motor penetrates down through a spindle sleeve A of the top plate to be connected with the I-shaped turning block A. an auxiliary rotating plate penetrates down through the spindle sleeve A of the top plateto be connected with the I-shaped turning block B. The I-shaped turning block A and the I-shaped turning block B are connected with the connecting band. The rotating connecting band drives the electric cylinder to rotate, and then the lifting frame rotates. Electrophoresis effect of parts suspended on the lifting frame is raised, and a liquid in an electrophoresis pool also can be stirred.
Owner:十堰市中仁汽车零部件有限公司

Fine electret particles and process for producing same

The present invention provides uniformly electrified electret fine particles having excellent electrophoretic properties. Specifically, the present invention provides a core-shell electret fine particle comprising a core part and a shell part, the core part containing a material that has an ability to disperse a pigment therein, the shell part containing an electret resin, and the electret resin being a fluorine-containing resin and being electretized by electron ray irradiation, radial ray irradiation, or corona discharge treatment.
Owner:SAKURA COLOR PRODUCTS CORPORATION

Surface rust removal treatment process for electrophoresis machining

ActiveCN113070279AAchieve the purpose of rust removal without dead endsImprove the effect of electrophoresisCleaning using liquidsElectrophoresesStructural engineering
The invention relates to the technical field of surface rust removal treatment, in particular to a surface rust removal treatment process for electrophoresis machining. A rust removal device used in the process comprises a rectangular pool, a discharging barrel, an ultrasonic generator and a controller; one side wall of the rectangular pool is rotationally connected with a rotating rod; one end of the rotating rod is hinged to a rotating shaft; the discharging barrel is arranged on the rotating shaft in a sleeving manner; the rotating shaft is located at the center axis of the discharging barrel and fixedly connected with the inner wall of the discharging barrel through a supporting rod; and an opening is formed in the end, away from the rotating rod, of the discharging barrel. According to the rust removal device used in the surface rust removal treatment process for electrophoresis machining, a workpiece is driven by the discharging barrel to rotate in the rectangular pool, and then ultrasonic waves emitted by an ultrasonic wave emitter are used in cooperation, so that the workpiece in the discharging barrel is subjected to rust removal while shaking, the purpose of dead-corner-free rust removal of the workpiece is achieved, the cleanliness of the surface of the workpiece is improved, and the electrophoresis effect of the workpiece is promoted.
Owner:佛山市顺德区锦上花涂装工艺有限公司

Modified copper phthalocyanine, preparation method, application and blue electronic ink

The invention discloses modified copper phthalocyanine, a preparation method, application and blue electronic ink. The modified copper phthalocyanine is prepared from copper phthalocyanine, ionic liquid and absolute ethyl alcohol. The ionic liquid is pyridine ionic liquid or pyrrolidine ionic liquid; the prepared modified copper phthalocyanine has good chromaticity and density, is uniform in size, and has excellent dispersion stability and electrophoresis performance in a non-polar medium, and the zeta potential of the modified copper phthalocyanine can reach more than + 50 mV; the modification method is physical adsorption, and the chromaticity and optical performance of pigment particles are reserved to the maximum extent; the preparation method is simple, short in time consumption and remarkable in effect; the blue electronic ink is prepared from modified copper phthalocyanine, tetrachloroethylene and Span80, the blue electronic ink is applied to electrophoretic display liquid, and tests show that under the bias voltage of 30V, the response time of a simple EPD device is 11121 ms, the maximum contrast ratio is 3.173, and the blue electronic ink shows huge practical application potential in color electrophoretic display.
Owner:UNIV OF ELECTRONICS SCI & TECH OF CHINA ZHONGSHAN INST

Girder reinforcing plate structure

The invention discloses a girder reinforcing plate structure, which comprises a rear girder assembly, a rear girder reinforcing plate and a rear floor assembly, wherein the rear girder reinforcing plate is positioned between the rear floor assembly and the rear girder assembly; a cavity is formed between the lower surface of the front end of the rear girder reinforcing plate and the upper surfaceof the front end of the rear girder assembly; the lower surface of the rear end of the rear girder reinforcing plate is fixed to the upper surface of the rear girder assembly; the upper surface of therear girder reinforcing plate is fixed to the lower surface of the rear floor assembly; and seats in the rear row of an automobile are detachably fixed to the front end of the corresponding rear girder reinforcing plate. According to the girder reinforcing plate structure, the lower surface of the front end of the rear girder reinforcing plate is separated from the upper surface of the front endof the rear girder assembly, electrophoresis liquid can be induced to enter the space between the rear girder reinforcing plate and the rear girder assembly, the electrophoresis effect is improved, and the corrosion resistance of an automobile body is improved.
Owner:SAIC-GM-WULING AUTOMOBILE CO LTD

High-strength antistatic 3D sheet and preparation method thereof

The invention discloses a high-strength antistatic 3D sheet and a preparation method thereof. The preparation method of the 3D sheet material comprises the following steps; the method comprises the following steps: (1) preparing a glue solution, and coating the surface of glass fiber cloth with the glue solution through an electrophoresis method to form a prepreg; (2) attaching a release film to the surface of the prepreg, slitting the prepreg into different shapes, stripping and removing the release film, and stacking the prepreg layer by layer to form a to-be-pressed prepreg; and (3) putting the prepreg to be pressed into a mold, and carrying out compression molding to obtain the high-strength antistatic 3D sheet. The 3D sheet prepared by the invention has good mechanical strength, excellent antistatic property, heat dissipation property and heat resistance, and can be widely applied to cover plates and protective jackets of mobile phones, communication equipment and other electronic products.
Owner:HUIZHOU ZONGSHENG ELECTRONICS MATERIAL CO LTD

A car body electrophoresis spreader

The invention discloses an automobile body electrophoresis sling including a sling support, the sling support is in a rectangular structure, the upper side of the sling support is provided with a sling chain, the lower side of one end of the sling support is provided with a front hanging arm, the lower side of the other end of the sling support is provided with a back hanging arm, a plurality of water pumps are arranged on the periphery of the upper side of the sling support, water inlet ends of the water pumps are connected with a water inlet pipe, the outer end of the water inlet pipe is fixedly connected to the bottom of the front hanging arm and the back hanging arm, water outlet ends of the water pumps are connected with a water outlet pipe, the outer end of the water outlet pipe faces towards the lower side of the sling support, the front hanging arm and the back hanging arm are also provided with chains, the outer ends of the chains are provided with connecting hooks. Therefore, the automobile body electrophoresis sling can maintain the automobile body to be suspended in electrophoretic liquid, the sling may not contacts with automobile body, the electrophoresis effect is good, at the same time automobile body process holes are not stressed, and the automobile body electrophoresis sling is not easy to damage.
Owner:ZHEJIANG GEELY HOLDING (GROUP) CO LTD +2

A hanger for electrophoresis

The invention relates to a hanging tool for electrophoresis. The hanging tool comprises a workpiece (5), an end cap (50), an upper piece frame (1) and a support (2), wherein the workpiece (5) is cylindrical; one end of the workpiece (5) is closed and the other end of the workpiece (5) is open; the open end of the workpiece (5) is sealed by virtue of the end cap (50); the workpiece (5) is clamped between the upper piece frame (1) and the support (2); the closed end of the workpiece piece (5) pushes against the upper piece frame (1); and the end cap at the open end of the workpiece piece (5) pushes against the support (2). The hanging tool for electrophoresis is suspended on a conveying machine of an electrophoresis production line and can be used for performing electrophoresis on the cylindrical workpiece; and the workpiece is fixed without moving during electrophoresis, and an electrophoresis solution cannot flow into the workpiece, so that the electrophoresis effect at the outer part of the workpiece is very good.
Owner:江苏舜天新盈轻工业有限公司

Agarose capsule and preparation method thereof

The invention discloses an agarose capsule, and relating to the field of agar production and application. The agarose capsule comprises a capsule shell and agarose. The raw material components of thecapsule shell comprise sucrose, maltose and honey, the capsule shell comprises an upper shell body and a lower shell body, the agarose is constructed into a plurality of agarose particles with fixed weight, and the upper shell body and the lower shell body can be connected in a sleeving manner so as to encapsulate all the agarose particles in the capsule shell. The capsule shell is prepared from sucrose, maltose and honey which are easy to obtain and low in cost, so that the cost of the agarose capsule is reduced; cane sugar and maltose are bonded through honey, so that low quality of the capsule shell caused by dehydration and condensation of the raw materials in the drying process is avoided; the agarose capsule is arranged in a form of sleeving the upper half body and the lower half body, so that agarose can be conveniently filled into the capsule, and the agarose capsule is simple to manufacture.
Owner:浙江诗韵医疗科技有限公司

Small-size workpiece electrophoresis tool

The invention discloses a small-size workpiece electrophoresis tool which comprises an electrophoresis frame; frameworks of the electrophoresis frame are closed through filter plastic nets; the filterplastic net on the upper side of the electrophoresis frame is detachably installed; a plurality of hook units, each of which comprises a cross rod, vertical rods and hangers, are horizontally arranged in the electrophoresis frame at intervals; each cross rod is horizontally arranged on the electrophoresis frame; a plurality of vertical rods, each of which is uniformly provided with a plurality ofhangers, are hung on each cross rod; three hooks are arranged in each hanger; an included angle between every two hooks is 120 degrees; a plurality of vertical columns are arranged on the electrophoresis frame along the side surfaces respectively; a plurality of cross rods are arranged between the corresponding symmetric vertical columns; the end of each cross rod is connected with the output endof an ultrasonic generator; a through hole is formed in each vertical column; an outer sleeve ring is in interference fitting to the interior of each through hole; an inner sleeve ring is placed in each outer sleeve ring; and a spring is arranged between each inner sleeve ring and the corresponding outer sleeve ring. The small-size workpiece electrophoresis tool provided by the invention has thebeneficial effects of good electrophoresis effect and high electrophoresis efficiency.
Owner:成都大运汽车集团有限公司

Saddle electrophoresis clamping device

The invention relates to a saddle electrophoresis clamping device which comprises a fixing table, a rotating motor is fixed to one side of the fixing table, the output end of the rotating motor penetrates through the fixing table and is fixedly provided with a first supporting plate, and a second supporting plate and a third supporting plate which are symmetrically distributed are fixed to the top end and the bottom end of the side, away from the rotating motor, of the first supporting plate; a plurality of evenly-distributed first clamping mechanisms are arranged on one side of the first supporting plate and used for clamping the outer surface of the saddle, and a plurality of evenly-distributed second clamping mechanisms are fixed to the upper surface of the third supporting plate and used for clamping the inner surface of the saddle; and the saddle electrophoresis clamping device solves the problems that in the using process of an existing saddle electrophoresis clamping device, clamping is not firm enough, a saddle is likely to fall off, the electrophoresis process is affected, meanwhile, the clamping mode is single, and large limitation exists.
Owner:SUSONG ANBAO BUSINESS MACHINE

Rotating electrophoresis tooling

The invention discloses a rotating electrophoresis tooling, which comprises an electrophoresis frame , wherein a rotating crawler belt (a front and back position rotating crawler belt) is arranged ineach of the front and back side positions of the electrophoresis frame; one installing block is fixed onto each crawler belt section of the rotating crawler belt; a transverse rod is arranged betweenthe front and back opposite installing blocks through a bearing; a vertical rod is hung on the transverse rod; a plurality of hooks are uniformly arranged on the vertical rod; the left and right endsof the rotating crawler belt are respectively sleeved on the rotating shaft; the rotating shaft is arranged on the electrophoresis frame through the support seat; the rotating shaft is also connectedwith an input motor through a belt; each surface of the electrophoresis frame is also sealed through a plastic net; in addition, the plastic nets are detachably installed. The rotating electrophoresistooling has the beneficial effect that the electrophoresis effect is good.
Owner:成都大运汽车集团有限公司

Liquid developer

[Problem] To provide a liquid developer for electrophotography or electrostatic recording, said developer having good electrophoretic properties and good transferability to print base materials. [Solution] Provided is a liquid developer obtained by dispersing colored resin particles in an insulating solvent, said colored resin particles comprising at least a pigment, a pigment dispersant, and a binder resin, and being obtained by coacervation, wherein the insulating solvent is mixed: in such a manner that the ratio of C11-12 aliphatic hydrocarbons is 8.0 to 20.0 mass% with respect to the total amount of the insulating solvent, the ratio of a C17-30 aliphatic hydrocarbon solvent is 2.0 to 45.0 mass%, and the total ratio of the C11-30 aliphatic hydrocarbon solvent is 99 mass% or higher; and in such a manner that the viscosity at 25 DEG C is 2.0 to 10.0 mPa*s.
Owner:SAKATA INX

Molecular method for determining virulence of phytophthora sojae on disease-resistant gene Rps3a(Chapman)

The invention relates to a molecular method for determining virulence of phytophthora sojae on a disease-resistant gene (i)Rps3a( / i)(Chapman), comprising the steps: extracting genome DNA of phytophthora sojae to be detected, carrying out PCR amplification to obtain a DNA fragment containing avirulence gene(i)Avr3a( / i), carrying out enzyme digestion with restriction enzyme (i)Bpu( / i)10I, carrying out agarose gel electrophoresis on the enzyme digestion product to obtain an agarose gel electrophoretogram of the enzyme digestion product; and observing the agarose gel electrophoretogram of the enzyme digestion product, if 2 DNA bands exist in a 250bp-500bp range, the corresponding phytophthora sojae strain to be detected is an avirulent strain, and if 1 DNA band exists under 750bp in the electrophoretogram after enzyme digestion, the corresponding phytophthora sojae strain to be detected is a virulent strain. The method has the advantages of short determination cycle, no intermediate type, accuracy and reliability, and can determine with a high flux reactor the virulence of phytophthora sojae on the disease-resistant gene (i)Rps3a( / i)(Chapman).
Owner:HENAN UNIV OF SCI & TECH

A surface derusting treatment process for electrophoresis processing

ActiveCN113070279BAchieve the purpose of rust removal without dead endsImprove the effect of electrophoresisCleaning using liquidsElectrophoresesEngineering
The invention relates to the technical field of surface derusting treatment, in particular to a surface derusting treatment process for electrophoresis processing. The derusting device used in the process includes a rectangular pool, a discharge cylinder, an ultrasonic generator and a controller; One of the side walls of the rectangular pool is rotatably connected to the rotating rod; one end of the rotating rod is hinged to the rotating shaft; the discharging cylinder is sleeved on the rotating shaft; The inner wall of the discharge cylinder is fixed; the end of the discharge cylinder away from the rotating rod is provided with an opening; the derusting device used in the surface rust removal process for electrophoresis processing according to the present invention drives the workpiece through the discharge cylinder. Rotate in the rectangular pool, and then cooperate with the ultrasonic waves emitted by the ultrasonic transmitter, so that the workpiece in the discharge barrel can be derusted while shaking, so as to achieve the purpose of derusting the workpiece without dead ends, so that the cleanliness of the workpiece surface is improved. Promotes the effect of workpiece electrophoresis.
Owner:佛山市顺德区锦上花涂装工艺有限公司

Electrophoresis auxiliary device for automobile parts production

The invention provides an electrophoresis auxiliary device for auto part production. The device comprises a corrugated rubber section, a belt wheel protecting hood, a functional rod and baffle pieces;the left and right end faces of an electrophoresis tank body are provided with a left working position upright frame and a right working position upright frame respectively, and the structures of thetwo working position upright frames are completely the same; the left and right working position upright frames are of sliding groove structures, and bearing seats which are used for achieving cooperative fixing of the two top ends of a lead screw are installed on the top faces and bottom faces of grooved cavities of the left and right working position upright frames; two first motor boxes are arranged, the baffle pieces are densely arranged on the functional rod so that work pieces hung on the two working positions can be moved when the first motor boxes are rotated to allow the work piece to be in a moving state swinging left and right, the electrophoresis effect is better, the electrophoresis speed is improved, vibration treatment can be conducted after electrophoresis coloring is completed, to-be-dried work pieces after electrophoresis is conducted are swung to contact with air to a further extent, rapid drying is convenient, and the working efficiency is improved.
Owner:湖南聚鑫科技有限公司

An electrophoretic spreader for automobile beams

The invention discloses an electrophoresis lifting tool for an automotive frame. The electrophoresis lifting tool comprises a bracket which is of an I-shaped structure; two iron chain mounting holes which are symmetric to a long shaft of the bracket are respectively fixedly connected to two ends of the upper surface of the bracket; a positioning rod assembly is fixedly connected below the bracket, comprising three groups of positioning frames which are of the same structure and in the shape of a Chinese character Wang (king), wherein the three groups of positioning frames are respectively fixedly mounted at the left, middle and right of the bracket; each positioning frame in the shape of the Chinese character Wang (king) comprises a plurality of positioning hinge pins; a plurality of circular holes which are uniformly linearly distributed are formed in the positioning hinge pins; a positioning pin is arranged on each positioning hinge pin; the positioning pins pass through the circular holes to be fixed to the positioning hinge pins. With the adoption of the lifting tool, the automotive frame can be stably suspended in an electrophoresis pond when in electrophoresis; the positioning pins cannot fall due to moving up and down in the electrophoresis pond; therefore, the electrophoresis effect is good; the separation with the lifting tool is avoided; the use safety is improved; the lifting tool is suitably widely popularized and used in the automobile production and manufacturing industry.
Owner:XINXIANG VOCATIONAL & TECHN COLLEGE

Molecular method for determining the pathogenicity of the Phytophthora soybean avirulence gene avr3a to the differential host l83-570

The invention relates to a molecular method for determining the pathogenicity of phythophthora sojae avirulent genes Avr3a to a differential host L83-570.The genome DNA of phythophthora sojae to be determined is extracted; DNA fragments including complete avirulent genes Avr3a are obtained through PCR amplification; DNA fragments are obtained through enzyme digestion and amplification of restriction endonuclease Bmg BI; the enzyme digestion product is subjected to agarose gel electrophoresis, wherein the corresponding phythophthora sojae strains to be determined have no pathogenicity to the differential host L83-570 if an agarose gel electrophoretogram is provided with two DNA bands within the range of 250 bp to 500 bp, and the corresponding phythophthora sojae strains to be determined have the pathogenicity to the differential host L83-570 if one DNA band exists within the range of below 750 bp after enzyme digestion.The determination period of the method is short, no intermediate form exists, and the pathogenicity of the multiple phythophthora sojae avirulent genes Avr3a to the differential host L83-570 can be simultaneously determined.
Owner:HENAN UNIV OF SCI & TECH
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