The invention provides a preparation method of a fluorescent powder ball mill. The preparation method of ball milled powder comprises the steps of, 1 raw material preparation, wherein the fluorescent powder ball mill, a PEO glue solution and an aluminum oxide dispersion solution are prepared independently in advance; 2, powder syrup preparation, wherein the prepared fluorescent powder ball mill is placed into a powder syrup preparation machine, then, the PEO glue solution, the aluminum oxide dispersion solution and recycling powder are added respectively, and sufficient mixing and stirring are performed; 3, powder syrup sampling. The manufacturing method of the fluorescent powder ball mill is improved, on the basis of the proper proportion and sufficient stirring, the fluorescent powder uniformity can be improved, the optical performance can be improved, waste can be recycled, the manufacturing utilization rate can be improved, and the production cost can be lowered.