Method for hydrolyzing silkworm cocoons through weak base via microwaves

Abstract

The invention discloses a method for hydrolyzing silkworm cocoons through a weak base via microwaves. The method comprises the following steps: (1) washing and drying; (2) cutting and separating; (3)carrying out primary hydrolysis and degumming; adding silkworm cocoon sheets and a weak base mixed solution formed by mixing sodium bicarbonate and sodium chloride into a microwave reactor; hydrolyzing for 110 to 130min under normal pressure and at the temperature of 80 DEG C; after hydrolyzing, obtaining a coarse sericin peptide solution and a silk protein fiber solid; pumping out the coarse sericin peptide solution and carrying out ultra-filtration to obtain sericin peptide protein; (4) carrying out secondary hydrolysis to extract silk fibroin peptide protein; then adding the weak base mixedsolution into the microwave reactor again; then hydrolyzing for 110 to 130min under normal pressure and at the temperature of 100 DEG C; carrying out heat insulation for 8 to 11h to completely hydrolyze the silk protein fiber solid into a coarse silk fibroin solution; carrying out ultra-filtration on the coarse silk fibroin solution to obtain silk fibroin protein. The method disclosed by the invention can be used for effectively preparing the sericin peptide protein and the silk fibroin protein in one step and the aim of improving the preparation efficiency and the utilization rate of products is realized.

Description

technical field [0001] The invention relates to the technical field of silk protein, in particular to a method for hydrolyzing silkworm cocoons with a weak alkali microwave. Background technique [0002] The silk industry originated in my country has a long history of thousands of years. However, the application of silk is mainly to use silk fiber as clothing material. With the progress of society and the development of modern science and technology, the development and utilization of new functions of silk are becoming more and more important. more and more become a trend. [0003] At the end of the 1920s, the silk industry experienced overproduction for the first time in the world. From 1933, people took the lead in applying silk to the field of non-clothing clothing and used it through research. Since then, the multifunctional development and utilization of silk protein has begun. It is found through research that silk is mainly composed of 20-30% sericin peptide protein a...

AI Technical Summary

Problems solved by technology

[0007] The above-mentioned patents can prepare sericin peptide protein or silk fibroin peptide protein well, but because sericin and silk fibroin complement each other, the above-mentioned patents can only prepare one kind of sericin peptide protein or silk fibroin peptide protein, and Can not prepare two kinds at the same time, it has defects such as low preparation efficiency and low product utilization rate

Moreover, both of the above two pieces used strong acid or strong base (sodium hydroxide) in the preparation process. After hydrolysis, it is necessary to neutralize the acid or base of the hydrolyzed liquid and physically extract the silk fibroin sericin peptide protein. Waste liquid treatment and discharge The amount is also increased, and the pollution control of the post-process is increased, and the efficiency of the process will be low if the process is increased.

Therefore, there are defects such as low preparation efficiency, high manufacturing cost, large pollution, and large protein damage, resulting in that the quality of the product cannot be guaranteed.