The invention discloses a sealing mechanism of an edge tool box for general practice. The sealing mechanism comprises a barrel body, a barrel cover, a first sealing rotating plate, a second sealing rotating plate and an inner lining plate, wherein the barrel cover is arranged at the top end of the barrel body, the first sealing rotating plate is arranged at the upper end of the barrel cover, the second sealing rotating plate is arranged at the lower end of the barrel cover, the lower end of the second sealing rotating plate is provided with the inner lining plate, the periphery of the inner lining plate is fixedly connected to the inner side of the upper end of the barrel body, a limiting block is fixedly connected between the inner lining plate and the barrel cover, one end of the limiting block is fixedly connected to the inner side of the barrel body, a plurality of groups of clamping connecting blocks are fixedly connected to one side of the limiting block, and corresponding clamping grooves are formed in the inner lining plate corresponding to the clamping connecting blocks. The sealing mechanism has the advantages that the structure is simple, the use is convenient, the sealing effect is good, and edge tools are not prone to falling off after sealing, and the sealing mechanism is mainly used for sealing treatment of the medical edge tools.