The invention discloses an isolation compartment device with negative pressure ventilation and epidemic prevention medical functions. The device comprises an isolation compartment, individual partitions, a stretcher door, an isolation door, a stretcher fixing position, a sterilizing lamp, a toilet, a negative pressure ventilation and disinfection system and a main valve, wherein the isolation doorand the stretcher door are respectively mounted at one side of the isolation compartment, the individual partitions, the stretcher fixing position, the sterilizing lamp and the toilet are mounted inthe isolation compartment, and a valve, a pump, a generator and the main assembly of the negative pressure ventilation and disinfection system are mounted in an equipment compartment. In addition, anoxygen delivery pipeline, a disinfectant pipeline and an exhaust pipeline which are connected with the negative pressure ventilation and disinfection system are respectively mounted in the isolation compartment, and the main valve is mounted on the oxygen delivery pipeline, the disinfectant pipeline and the exhaust pipeline. The isolation compartment device with negative pressure ventilation and epidemic prevention medical functions, disclosed by the invention, is good in air tightness and reasonable in arrangement and has the properties of high efficiency, multiple filtration and efficient filtration; and the equipment is independently partitioned to ensure that the inside of the compartment is easy to sterilize, disinfect and clean, the exhausted gas is not polluted, and a vehicle is clean when the vehicle is used every time.