31results about How to "Guaranteed normal culture" patented technology

The invention discloses a solar spectral photosynthetic bioreactor system for culturing microalgae in high density, which comprises a photosynthetic bioreactor, a solar energy collector, light-guide fibers, a light distribution device arranged in the photosynthetic bioreactor, a residual gas absorption device and a culture solution separation recovery device, wherein the residual gas absorption device and the culture solution separation recovery device are respectively connected with the photosynthetic bioreactor. One end of the light distribution device is connected with a spectral light-intensity adjustment device arranged on the photosynthetic bioreactor; the spectral light-intensity adjustment device is connected to the solar energy collector through the light-guide fibers; and a gas distribution device is arranged between the position below the light distribution device and the bottom of the photosynthetic bioreactor and is connected with the output end of a gas mixing device. The system can effectively improve the utilization ratio of solar energy, reduce the consumption of external electrical energy, solve the problems of solar energy utilization, such as intermittence, instability difficult collection, and ensure the continuous and stable culture of microalgae.

The invention discloses a 3D culture, passage, cryopreservation, recovery and identification method for in-vitro organoids based on small intestines of different segments of mice. The method comprisesthe following steps: (1) separating recesses of duodenum, jejunum and ileum segments of mice; (2) performing 3D culture on the recesses of the duodenum, jejunum and ileum segments of mice, and forming organoids; (3) performing passage on organoids of small intestines of mice; (4) performing cryopreservation on the organoids of small intestines of mice; (5) performing recovery on the organoids ofsmall intestines of mice; (6) preparing frozen sections of the organoids of small intestines of mice; and (7) performing immunofluorescence labeling and identification on the frozen sections of the organoids of small intestines of mice. Compared with the prior art, the method disclosed by the invention optimizes a recess extraction manner, an in-vitro culture system and a culture medium based on the recesses of the small intestines containing stem cells, and comprises the steps of complete culture, passage, cryopreservation, recovery and identification. The small intestine organoids obtained by the method are capable of performing repeated passage, and the passage organoids have character stability.

The invention provides tissue cryopreservation liquid capable of maintaining cell activity, comprising trehalose, dimethyl sulfoxide, and serum substitute KSR (knockout serum replacement). After resuscitation of tissue preserved with the inventive cryopreservation liquid, the obtained adipose-derived stem cells (ADSCs) have the advantages of good anchorage growth, high purity, and strong differentiation ability.

The invention relates to a tissue engineering myocardium bioreactor constructed by pouring, perfusion and pulsation combination, belonging to the technical field of tissue engineering and biological engineering manufacture. The bioreactor comprises a cell pouring generator, a perfusion module, a pulsation generator, a culture module and a control module; the cell pouring generator, the perfusion module and the pulsation generator are communicated with the culture module through pipelines; and the culture module comprises a pulsation cavity, a pulsation effect soft tube with one closed end and a perfusion cavity, and the perfusion module and the pulsation generator carry out perfusion culture and pulsation training. The invention can improve cardiomyocytes planting depth and simulate myocardium in vivo environment, and carries out culture to obtain a tissue engineering myocardium with larger thickness and autonomously beating capacity. The bioreactor is used for realizing that the tissue engineering myocardium construction carries out perfusion culture and pulsation training simultaneously or separately on cell perfusion and tissue culture stages in a cell planting stage.

The invention relates to a novel microbial inspection incubator, which comprises an incubator body, an incubator door, partition boards, universal wheels, an air outlet, air inlets, fluorescent lamps, heating wires, temperature sensors, a ventilating fan, an operating keyboard, a handle and a viewing window, wherein the air outlet is formed in the top of the incubator body; the ventilating fan is arranged on the air outlet; by virtue of the partition boards, the interior of the incubator body is equally divided into three layers; one air inlet is each formed in the left and right sidewalls of the incubator body in each layer; the fluorescent lamps are arranged on two side ribs of the incubator body in each layer; the temperature sensor is arranged on the left sidewall of the incubator body in each layer and the temperature sensor is connected to a control circuit; the heating wire is arranged on the rear wall of the incubator body in each layer and the heating wire is connected to the control circuit; the universal wheels are arranged at four corners of the bottom of the incubator body; the operating keyboard is arranged at the upper side of the incubator body; the viewing window is arranged in the middle of the incubator door; and the handle is arranged at the left side of the viewing window. The incubator designed by the invention is convenient to clean and convenient to operate.

The invention belongs to the field of super rice cultivation and discloses a double cropping super rice high-yield cultivation method. The double cropping super rice high-yield cultivation method comprises selecting planting fields, and ploughing and sterilizing the fields; spraying lime and special farmyard manure onto the fields; determining a species to be cultivated, and breeding rice seedlings; steeping the fields and applying base fertilizer; after the rice seedlings are transplanted, ensuring sufficient water for seedling reviving; applying weeding fertilizer; during a tillering stage,mainly performing moisture management, and during young ear formation and heading-flowering stages, performing shallow water irrigation. According to double cropping super rice high-yield cultivationmethod, the applied fertilizer application method saves processes of three fertilizer application and three pesticide application; the double cropping super rice high-yield cultivation method is simple, concise, precise in fertilizer and pesticide application and simple in operation and is a concise cultivation technique; during cultivation of the rice seedlings, bactericides are sprayed onto thesurface of nutrient soil to effectively improve the sterilizing effects and create a good environment for rice growth; rice seeds are soaked into dilute saline and then into bactericides to thoroughlykilling carried bacteria, moulds and the like and accordingly to improve the sprouting rate and the strong sprout rate of the rice seeds.

The invention relates to the field of microbiological analysis and detection, and particularly discloses a method for detecting clostridium perfringens in drinking natural mineral water. The inspection method comprises the following steps: a, filtering a water sample by adopting a filter membrane, then tightly placing the filter membrane on a bottom culture medium, paving a middle culture medium on the filter membrane, carrying out anaerobic inverted culture for 24-26h at the temperature of 35-37 DEG C, and counting the number of black bacterial colonies; and b, picking 3-5 black bacterial colonies, respectively inoculating the black bacterial colonies on a liquid thioglycolate culture medium, culturing for 18-24 hours at the temperature of 35-37 DEG C, and taking the black bacterial colonies to carry out a confirmation test. By means of the detection method, the black characteristic of the cultured bacterial colony is obvious, identification is easy, and the accuracy of the detection result is high.

The invention discloses an edible mushroom factory fresh air pre-processing system with high air cleanliness. The edible mushroom factory fresh air pre-processing system comprises a house, a passage arranged in the middle of the house, mushroom growing rooms arranged on the two sides of the passage inside the house, air conditioner internal machines arranged at the upper ends of the mushroom growing rooms, and exhaust fans arranged at the lower ends of the mushroom growing rooms. An air tight chamber is arranged at the upper end of the passage, one side of the air tight chamber is provided with an air inlet, an efficient filtering net is arranged at the air inlet, the two sides of the air tight chamber are provided with fresh air machines respectively, a humidifier is arranged inside the air tight chamber, and an air pre-processing device is arranged on the part, outside the air inlet, in the house. The air pre-processing device structurally comprises a filtering chamber arranged on the part, outside the air inlet, of the house, and air inlets in the two sides of the filtering chamber are provided with filtering nets respectively.

The invention relates to a bamboo-stump cultivation method for wild Ganoderma lucidum. Ganoderma lucidum cultivation is carried out through carrying out culture medium preparation, carrying out mycelium culture, carrying out environment setting, carrying out Ganoderma lucidum fruiting body cultivation and carrying out spore powder collection. The method has the beneficial effects that the Ganoderma lucidum cultivation is carried out through selecting bamboo stumps, remaining after bamboos in wild bamboo groves are hewn, as carriers, wild natural-environment cultivation is achieved, and Ganoderma lucidum absorbs wild natural nutritional ingredients and is abundant in nutritional ingredient content, so that the formation state of effective ingredients of Ganoderma lucidum bodies is good, and cultivated Ganoderma lucidum can have the effects of wild Ganoderma lucidum; the medicinal value is high, and the cultivation process is simple, so that the problems of the artificially-cultivated Ganoderma lucidum at present that the medicinal value is low and the cultivation process is complicated are solved, and the yield of Ganoderma lucidum can also be increased; and spore powder can be extensively collected, so that the income of Ganoderma lucidum cultivation is increased, the economic benefit is increased, the heavy demand of markets are met, and the popularization is facilitated.

The invention discloses a simplified steel frame insect-proof net non-toxic seedling raising technology for Longhuihong navel oranges, relates to a cultivating technology for navel oranges, and in particular relates to a seedling raising technology for Cara Cara navel oranges. The technology can stably and efficiently ensure seedling culture of the Longhuihong navel oranges to meet market needs. The technology comprises the following steps: 1, nursery preparation; 2, female parent cultivation; 3, rootstock seedling breeding; 4, grafting; and 5, small-seedling tending. The technology is characterized in that the nursery preparation includes the steps of nursery land selection, land preparation, construction of an insect-proof net greenhouse, ditching for discharging water, cultivation of stock trees, and cultivation of scion trees. According to the technology disclosed by the invention, the simplified steel frame insect-proof net greenhouse is arranged, selection of a non-toxic female parent cooperates, the Longhuihong navel oranges are cultivated by using a seedling seal cultivation manner to make seedlings live through the low-immunity seedling stage, site selection criteria, thefemale parent cultivation, the rootstock seedling breeding, a grafting method and the small-seedling tending are standardized, so that the seedlings of the Longhuihong navel oranges realize quick cultivation, have a high survival rate and meet rapid promotion in a market.

The invention discloses a bacterial culture and storage device applied to a high-humidity environment, and belongs to the technical field of bacterial culture.According to the scheme, in the bacterial culture process, a humidity monitoring mechanism monitors the humidity change in a sterile culture box through a humidity sensor, and if the humidity is too high, the sterile culture box is started; the air-drying moisturizing mechanism pulls a moving plate and a sealing plug to be far away from an air outlet through an electric telescopic rod, so that hot air accumulated in a heat conduction ball is released, discharged upwards under pulling of the moving plate and flows through the inner side and the outer side of the sterile incubator, the humidity in the sterile incubator is reduced, and humidity balance is kept; the liquid cooling humidity supply mechanism sprays potassium nitrate powder through an electric spray head, the potassium nitrate powder is dissolved in an aqueous solution, the temperature is reduced, water vapor in the monitoring cabin is liquefied when cooled, air is promoted to keep moist, under pushing of an extrusion push block, the moist air is extruded oppositely to flow, the dryness of the sterile incubator is reduced, and a constant-humidity environment is created. Growth and reproduction of bacteria are promoted, and the culture effect is enhanced.

The invention discloses a supporting mold made through a laser carving method. A mold frame comprises a supporting frame mold and a fixing frame mold with a groove. The frame mold is rectangular and is made of polymethyl methacrylate (PMMA), and a bonding agent is trichloromethane. The fixing frame mold with the groove is made of glass. Compared with the prior art, the culture supporting frame canadapt to culture of artificial blood vessels different in diameter, and it is guaranteed that the blood vessels are cultured under the dynamic conditions. Kinetic study research, tumor cell migrationand medicine diffusion tests of the in-vitro blood vessels and other research can be conducted.

The invention discloses a method for culturing airway epithelial cells by simply, conveniently and rapidly inducing differentiation of cilia. The method comprises the steps of separating in-vivo airway tissues from mammals, rinsing the airway tissues, longitudinally shearing and separating the airway tissues to form airway rings, tiling the annular airway tissues in a culture vessel, and putting the culture vessel into an incubator of 37 DEG C for culturing. The technical method adopted in the invention is simple, convenient and rapid, the operation procedure is simplified, the culture period is shortened, meanwhile, cultured cells can differentiate a great number of cilia, and a great number of cells can be obtained by virtue of a culture process for researching or other requirements.

The invention discloses cultivation equipment and cultivation method for edible wood-rotting fungi, and relates to the technical field of cultivation shelves. The cultivation equipment comprises a left positioning frame, a right positioning frame is arranged on the right side of the left positioning frame, a movable cross rod is fixed between the left positioning frame and the right positioning frame through bolts, a fixing plate is welded and fixed at the lower end of the left positioning frame and the right positioning frame, a sliding frame is slidably connected to the surface of a movable cross rod, and a containing device is clamped to the outer surface of the left positioning frame and the outer surface of the right positioning frame. The containing device is provided, through fixing teeth and a rotating disc on the surface of the containing device, the containing devices can be adjusted according to the sizes of the edible fungi, more edible fungi can be placed in the cultivation equipment, meanwhile, the distance between the containing devices can be changed when the edible fungi with high ventilation requirements are cultured, and normal cultivation of the edible fungi is guaranteed.

The invention discloses a multifunctional pathology incubator which comprises an incubator body, a culture frame, an incubator body handle, air leakage openings, a partition plate and a temperature controller. The partition plate is arranged in the incubator body, the incubator body is divided into two layers by the partition plate, the temperature controller is arranged at the upper end of the partition plate, a function lamp is arranged at the upper end of the incubator body, a limit groove is formed in the right side of the incubator body, sliding grooves are formed in the front end and therear end of the culture frame, sliding wheels are arranged at the front ends of the sliding grooves, the sliding grooves are rotatably connected with the sliding wheels, the incubator body is slidably connected with the sliding grooves in the culture frame through limit grooves, a baffle plate is arranged on the right side of the culture frame, limit holes are formed in the baffle plate, limit blocks are arranged on the right side of the incubator body, limit projections are arranged on the limit blocks, a culture frame handle is arranged on the right side of the baffle plate, the air leakageopenings are formed in the incubator body, different research objects are cultured through an upper layer and a lower layer in a classified manner, so that different conditions can be dealt, the culture frame 12 is slidably connected with the incubator body 1, taking is facilitated, and the incubator is convenient to carry by the aid of an integrated structure.

The invention belongs to the technical field of thallus culture and particularly discloses thallus culture equipment and method. The equipment and the method are used for solving the problems that the existing thallus culture equipment is complicated in structure and is not beneficial to timely separation of thalli. According to the thallus culture equipment, a first chamber, a second chamber and a third chamber which are sequentially connected from top to bottom are formed in a fermentation tank, thalli obtained through fermentation in the first chamber can be enriched through the second chamber, and the thalli enriched in the second chamber can be fully gathered and settled through the third chamber, so that the thalli can be concentrated without additionally arranging devices such as a rotary settling tank, a circulating pump and a circulating pipe, and the equipment structure is simplified; and meanwhile, a turbidity detection device is arranged in the third chamber, so that the turbidity of liquid in the third chamber can be detected in real time; and a liquid inlet of a centrifugal device is connected with a liquid outlet in the lower end of the third chamber through a vertical pipeline, so that materials can be discharged into the centrifugal device in time after the turbidity requirement is met, and the materials can be subjected to rapid centrifugal separation to obtain thallus activated sludge.

The invention discloses a suction method and an adding method of a biological tissue culture solution and relates to the technical field of biological tissue culture. The suction method comprises the following steps: extracting a negative pressure from a culture solution suction head, moving down the culture liquid suction head according to a first set distance, detecting a first state negative pressure in the culture solution suction head, and comparing the first state negative pressure with a first set negative pressure; if the first state negative pressure is greater than or equal to the first set negative pressure, stopping moving down the culture solution suction head and stopping extracting the negative pressure from the culture solution suction head, moving the culture solution suction head to a level of the culture solution, and discharging the solution in the culture solution suction head; moving down the culture solution suction head to a position below the level of the culture solution according to a second set distance and extracting a set amount of the culture solution; and after the culture solution is sucked, moving the culture solution suction head upwards and out of a culture solution bottle, and thus automatically sucking the biological tissue culture solution. The adding method comprises steps of uncovering, solution adding and covering. The biological tissue culture solution can be automatically added and a requirement for culturing biological tissues in batches is met.

The invention discloses a cell culture device for medical examination. The cell culture device comprises a box body, four supporting legs which are fixedly mounted at four corners of the bottom end ofthe box body correspondingly, a transparent plate which can be arranged at the middle of the front side of the box body in an opening and closing mode in the vertical direction, a temperature controller which is fixedly mounted at the top end of the front side of the box body, a sealing mechanism which is fixedly mounted on the front side of the bottom end of the transparent plate, a base which is fixedly arranged at the bottom end of the inner cavity of the box body, and supporting rods, wherein every two of the four supporting rods form a group, and the four supporting rods are fixedly mounted at the left end and the right end of the front side and the rear side of the base in the vertical direction. The cell culture device for medical examination is relatively complete in structure, relatively high in space utilization rate and relatively small in taking outlet, the contact area and time between the cell culture device and the external environment are reduced, the cell culture environment is further guaranteed, and cell culture is facilitated.

The invention discloses an edible mushroom factory fresh air system. The edible mushroom factory fresh air system comprises a house, a passage arranged in the middle of the house, mushroom growing rooms arranged on the two sides of the passage inside the house, air conditioner internal machines arranged at the upper ends of the mushroom growing rooms, and exhaust fans arranged at the lower ends of the mushroom growing rooms. An air tight chamber is arranged at the upper end of the passage, one side of the air tight chamber is provided with an air inlet, an efficient filtering net is arranged at the air inlet, and the two sides of the air tight chamber are provided with fresh air machines respectively. The edible mushroom factory fresh air system has the advantages that the air inside the air tight chamber is similar to the air in the mushroom growing rooms, the air cleanliness is improved, the frequency of washing the fresh air machine filtering net is reduced, a controller is used for controlling the fresh air machines and is arranged inside the air tight chamber, the edible mushroom factory fresh air system looks more attractive, the fresh air machines can provide fresh air for the mushroom growing rooms, normal cultivation and growth of edible mushrooms can be guaranteed, and the pollution to fungus spores from the natural environment is reduced.

The invention belongs to the technical field of shaking incubators, in particular to a three-color light multi-section adjustable system. The three-color light multi-section adjustable system comprises an incubator, a cabinet door which is mounted on the incubator and is rotatably connected through a rotating shaft, and an observation window which is formed in the surface of the cabinet door and is used for observing the internal state of the incubator, a control panel is installed on the surface of the incubator, an LED illumination plate is arranged in the incubator, and a plurality of LED lamps are arranged on the surface of the LED illumination plate; the problem of culture difference at different positions is solved; the intensity of light sources around the illumination plate is adjusted; the illumination intensity around and in the middle is the same; the three light sources are ensured to be selectable or combinable, actual culture is met, multiple sections can be independentlyor simultaneously arranged, the use is convenient, the illumination intensity is adjustable, different culture requirements are met, a unique heat dissipation system is adopted, the temperature riseis low, the influence on culture is small, the actual problem can be effectively solved through the above points, and the actual requirements of users are met.

The invention relates to a bamboo-stump cultivation method for wild Ganoderma lucidum. Ganoderma lucidum cultivation is carried out through carrying out culture medium preparation, carrying out mycelium culture, carrying out environment setting, carrying out Ganoderma lucidum fruiting body cultivation and carrying out spore powder collection. The method has the beneficial effects that the Ganoderma lucidum cultivation is carried out through selecting bamboo stumps, remaining after bamboos in wild bamboo groves are hewn, as carriers, wild natural-environment cultivation is achieved, and Ganoderma lucidum absorbs wild natural nutritional ingredients and is abundant in nutritional ingredient content, so that the formation state of effective ingredients of Ganoderma lucidum bodies is good, and cultivated Ganoderma lucidum can have the effects of wild Ganoderma lucidum; the medicinal value is high, and the cultivation process is simple, so that the problems of the artificially-cultivated Ganoderma lucidum at present that the medicinal value is low and the cultivation process is complicated are solved, and the yield of Ganoderma lucidum can also be increased; and spore powder can be extensively collected, so that the income of Ganoderma lucidum cultivation is increased, the economic benefit is increased, the heavy demand of markets are met, and the popularization is facilitated.

The invention discloses a simple and efficient unicellular algae separation and purification method, which comprises the following steps: accurately measuring the unicellular algae cell density of an algae solution to be separated and purified by using a blood cell counting plate under an optical microscope, diluting the algae solution to the unicellular algae cell density of 20-30 cells / mL by using sterilized sand filtration seawater, sucking 1-3 drops of diluted algae liquid according to the quantity of miscellaneous algae cells and harmful organisms in the algae liquid to be separated and purified, dripping the diluted algae liquid into a glass test tube filled with a unicellular algae culture solution, culturing for 10-15 days in a place with sufficient illumination and without direct sunlight, and carrying out microscopic examination and screening to obtain purified unicellular algae without miscellaneous algae cells and harmful organisms. The method disclosed by the invention is simple to operate, extremely low in requirement on professional degree of operators, wide in application place, suitable for laboratories with good conditions and farms with simple conditions, and capable of ensuring pure culture and long-term storage of the unicellular algae, and the success rate of separation and purification reaches 100%.

The invention belongs to the technical field of flora culturing, and discloses a preparation system of nematode No.1 battlefront flora. The system comprises a culturing module which is a device used for culturing nematode No.1 battlefront bacteria, a storage module which is used for the storage of the nematode No.1 battlefront bacteria and connected with the culturing module, a water injection module used for adding water into the storage module, a supplying module used for adding nutrient solution to the storage module and controlling the oxygen dissolving concentration of the storage moduleand a drainage module used for performing regular outward water drainage, and a detection module used for detecting the culturing and storing process and an alarm module used for giving prompts and alarms, wherein the water injection module, the supplying module and the drainage module are connected with the storage module, the detection module and the alarm module are connected with the culturingmodule and the storage module. According to the system, an automation technology is applied to the culturing process of the flora, the nematode No.1 battlefront flora can be effectively cultured, theoperation is simple and convenient and the culturing efficiency is high.

The invention provides tissue cryopreservation liquid capable of maintaining cell activity, comprising trehalose, dimethyl sulfoxide, and serum substitute KSR (knockout serum replacement). After resuscitation of tissue preserved with the inventive cryopreservation liquid, the obtained adipose-derived stem cells (ADSCs) have the advantages of good anchorage growth, high purity, and strong differentiation ability.

The invention discloses a cell culture transit insulation device, comprising a box body, a box cover, a lock, and an insulation layer arranged in the box body and the box cover. The lock is located on the front of the box body, a self-balancing component is movably arranged in the box body, an air circulation component is arranged on the top of the box cover, and a temperature control component is arranged at the bottom of the box body. In the present invention, by setting the self-balancing component, it can ensure that the cell culture dish placed in the placing box is always in a horizontal state, thereby ensuring the stability of cell transport, and the weight of the first battery pack, the motor and the rotor is larger than that of the culture dish and the culture dish. The weight of the cell, and the heavier end is located below the axis of the movable shaft, which further improves the stability of the culture dish when the box is tilted; when the box shakes or turns over, the motor drives the rotor to rotate to ensure that the culture dish is in relative level.