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Thallus culture equipment and method

A technology for culturing equipment and cells, applied in biochemical equipment and methods, methods for sampling biological materials, biochemical instruments, etc., can solve problems such as unfavorable cell separation, complex structure, etc., to improve convenience, production efficiency, and simplification. Equipment structure, the effect of improving product yield

Pending Publication Date: 2021-12-17
SICHUAN ACAD OF FOOD & FERMENTATION INDS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a bacterium culture equipment, aiming to solve the problem that the existing bacterium culture equipment has a complex structure and is not conducive to the timely separation of bacteria

Method used

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  • Thallus culture equipment and method

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Experimental program
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Effect test

Embodiment 1

[0038] Utilize the thalline culture equipment and the thalline culture equipment method provided by the present invention to continuously cultivate yeast;

[0039] In this example, the fermenter used has a total volume of about 650 L, the cylindrical body of the first chamber 1 has a height of 75 cm and a diameter of 80 cm, and the height of the inverted conical bottom of the first chamber 1 is 20 cm , the lower end diameter is 35cm; the height of the second chamber 2 of inverted conical shape is 100cm, and the upper and lower end diameters are respectively 80cm and 10cm; the height of the third chamber 3 of inverted conical shape is 53cm, and the diameter of the upper end is 42cm; the first The respective volumes of the chamber 1, the second chamber 2 and the third chamber 3 respectively account for about 66%, 30% and 4% of the total volume of the fermenter.

[0040] During cultivation, first pump 500L of yeast wort culture medium into the fermenter, and sterilize it, then in...

Embodiment 2

[0046] Utilize the thalline culture equipment and the thalline culture equipment method provided by the present invention to continuously cultivate Lactobacillus plantarum;

[0047] In this embodiment, the total volume of the fermentor used is about 2500L, the height of the cylindrical body of the first chamber 1 is 1m, and the diameter is 1.36cm, and the height of the inverted conical bottom of the first chamber 1 is 30cm, the lower end diameter is 43cm; the height of the second inverted conical chamber 2 is 107cm, the upper and lower end diameters are respectively 136cm and 36.5cm; the height of the third inverted conical chamber 3 is 57cm, and the upper end diameter is 57cm; The respective volumes of the first chamber 1 , the second chamber 2 and the third chamber 3 respectively account for about 68%, 30% and 2% of the total volume of the fermenter.

[0048] When cultivating, first pump 2000LMRS medium into the fermenter and sterilize it, then insert the seed liquid of Lact...

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Abstract

The invention belongs to the technical field of thallus culture and particularly discloses thallus culture equipment and method. The equipment and the method are used for solving the problems that the existing thallus culture equipment is complicated in structure and is not beneficial to timely separation of thalli. According to the thallus culture equipment, a first chamber, a second chamber and a third chamber which are sequentially connected from top to bottom are formed in a fermentation tank, thalli obtained through fermentation in the first chamber can be enriched through the second chamber, and the thalli enriched in the second chamber can be fully gathered and settled through the third chamber, so that the thalli can be concentrated without additionally arranging devices such as a rotary settling tank, a circulating pump and a circulating pipe, and the equipment structure is simplified; and meanwhile, a turbidity detection device is arranged in the third chamber, so that the turbidity of liquid in the third chamber can be detected in real time; and a liquid inlet of a centrifugal device is connected with a liquid outlet in the lower end of the third chamber through a vertical pipeline, so that materials can be discharged into the centrifugal device in time after the turbidity requirement is met, and the materials can be subjected to rapid centrifugal separation to obtain thallus activated sludge.

Description

technical field [0001] The invention belongs to the technical field of bacterium cultivation, and in particular relates to a bacterium cultivation equipment and method. Background technique [0002] Bacterial culture equipment is a facility and equipment that provides a suitable environment for bacterial growth based on the principle of biological fermentation. At present, people use more high-density culture methods, that is, optimize culture conditions, supplement nutrients and oxygen, or remove inhibitory factors to increase the amount of bacteria in a single batch of fermentation to obtain higher substrate utilization efficiency or Product yield. [0003] Since the bacteria are separated from the fermenter in time during the fermentation process, excessive consumption of nutrients and waste generation can be reduced, the growth time of the bacteria can be prolonged, and efficient fermentation results can be obtained. Therefore, the bacteria culture equipment includes fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/26C12M1/02C12M1/00C12N1/00C12N1/04
CPCC12M23/02C12M23/34C12M27/02C12M33/22C12M41/36C12N1/00C12N1/04
Inventor 张其圣范智义吕鹏军伍亚龙杨凯史梅莓
Owner SICHUAN ACAD OF FOOD & FERMENTATION INDS
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