Active dry saccharomyces boulardii feed additive and its preparation method and use
A technology for yeast feed and Boula yeast, applied in the directions of microorganism-based methods, biochemical equipment and methods, applications, etc., can solve the problems of low fermentation level, low number of viable bacteria in the product, low price, etc., and achieve low moisture content. , to ensure the effect of cultivation and fermentation effect
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Embodiment 1
[0030] Under sterile conditions, the Saccharomyces boulardii strains were inoculated into the activation medium containing 2wt% glucose, 0.5wt% peptone, 0.5wt% yeast extract powder and 1.5wt% agar, at 30°C The activated seed liquid was obtained by activating and culturing in medium for 52 hours. The activated seed solution is inoculated into the primary seed tank filled with the primary medium, the primary medium contains corn soaking water with 4wt% amino nitrogen, 0.5wt% ammonium sulfate, glucose crystallization mother liquor with a glucose value of 7wt%, phosphoric acid Potassium dihydrogen 0.2% and magnesium sulfate 0.05%, the amount of corn soaking water is 1 / 2 of the primary seed tank, the pH value of the primary medium is 4.5, the initial glucose content is 8%, and the primary expansion cultivation During the process, the ventilation ratio was kept at 0.5:1, the dissolved oxygen was controlled to 20% by stirring, and the temperature was cultivated at 30°C for 20 hours t...
Embodiment 2
[0034] Under sterile conditions, the Saccharomyces boulardii strains were inoculated into the activation medium containing 4wt% glucose, 0.7wt% peptone, 0.7wt% yeast extract powder and 2.5wt% agar, at 32°C The activated seed liquid was obtained by activating and culturing for 42 hours. The activated seed solution is inoculated into the first-level seed tank filled with the first-level medium, and the first-level medium contains corn soaking water with 5wt% amino nitrogen, ammonium sulfate 0.7wt%, glucose crystallization mother liquor with a glucose value of 8wt%, phosphoric acid Potassium dihydrogen 0.5% and magnesium sulfate 0.07%, the amount of corn soaking water is 1 / 2 of the primary seed tank, the pH value of the primary medium is 5.5, the initial glucose content is 12%, and the primary expansion cultivation During the process, the ventilation ratio was kept at 0.8:1, the dissolved oxygen was controlled to 30% by stirring, and the temperature was cultivated at 32°C for 15 ...
Embodiment 3
[0038] Under sterile conditions, the Saccharomyces boulardii strains were inoculated into the activation medium containing 3wt% glucose, 0.6wt% peptone, 0.6wt% yeast extract powder and 2.0wt% agar, at 31°C The activated seed solution was obtained by activating in medium for 48 hours. Inoculate the activated seed solution into the first-level seed tank filled with the first-level medium. The first-level medium contains corn soaking water with 4.5wt% amino nitrogen, 0.6wt% ammonium sulfate, and glucose crystallization mother liquor with a glucose value of 7.5wt%. , potassium dihydrogen phosphate 0.4% and magnesium sulfate 0.06%, the amount of corn soaking water is 1 / 2 of the primary seed tank, the pH value of the primary medium is 5.0, and the initial glucose content is 10%. During the expansion process, the ventilation ratio was kept at 0.7:1, the dissolved oxygen was controlled to 25% by stirring, and the temperature was cultivated at 31°C for 18 hours to obtain a primary cult...
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