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Method for testing clostridium perfringens in drinking natural mineral water

A technology for Clostridium perfringens and inspection methods, which is applied in the directions of microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc. Can not fully guarantee anaerobic conditions, reduce the accuracy of test results and other problems, to achieve the effect of reducing the possibility of false negative results, reducing the possibility and high accuracy

Pending Publication Date: 2021-04-06
河北安普检测技术服务有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, because Clostridium perfringens needs to be cultured under anaerobic conditions, in the actual inspection process, anaerobic conditions cannot be completely guaranteed, and oxygen may be introduced during the observation of colonies, etc. Colonies without obvious black features are produced, and the colonies will be judged as negative according to the standard requirements and the verification experiment will not continue, resulting in false negative results, resulting in missed detections, which cannot truly reflect the gas pods in the mineral water samples The presence of Clostridium membranes greatly reduces the accuracy of the test results

Method used

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Examples

Experimental program
Comparison scheme
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preparation example

[0038]The following preparation examples, embodiments, and contractions:

[0039]0.22μm filter is purchased from Germany Merck Mid Mingbo, batch number: F8EA35906;

[0040]0.45μm filter was purchased from Germany Merck Mid Mingbo, batch number: F8HA73482;

[0041]TSC Cultivate Substance from Beijing Luqiao Technology Co., Ltd., batch number: 180427;

[0042]Protein Water Posted from Qingdao Hubbo Bio Co., Ltd., Item No .: HB5218-1;

[0043]Truncinsence Caserkin is purchased from Huaan Biotechnology Group in Gansu;

[0044]Polymyxin B Sulfate is purchased from Shenzhen Sakko Biotechnology Co., Ltd., Item No .: 0319-100MU;

[0045]Blade Tianqing, from Beijing Zhongsheng Ruitai Technology Co., Ltd.

preparation example 1

[0047]The SPS medium is prepared by using the following method:

[0048]At a temperature of 100 ° C, 15 g of a trifase, 10 g yeast paste, 0.5 g of ferric ammonium and 15 g agar was dissolved in 1000 mL of distilled water, and the pH was 7.0, and then at a temperature of 121 ° C, the pressure was 103.4. Under the conditions of KPa, high pressure was sterilized for 15 min, then cooled to 50 ° C, 5 ml of a solution of sodium sulfite solution of 100 g / L, 10 ml concentration of 1.2 g / L, a polymyxin B sulfate solution and 10 ml concentration of 12g / L Sulfadiazine sodium aqueous solution is shaken, and the plate is poured.

preparation example 2

[0050]Liquid sulfuric salt (FT) medium is prepared by using the following method:

[0051]At 105 ° C temperature, 15 g of trunca, 0.5 g of L-cystine, 5 g glucose, 5G yeast paste, 2.5 g sodium chloride, 0.5 g of sodium sulfide, 0.001G blade, 0.75 G agar is dissolved in 1000 mL of distilled water, the pH is adjusted to be 7.1, and then at a temperature of 121 ° C, the pressure is 103.4 kPa, high pressure is sterilized for 15 min, cooled to room temperature.

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Abstract

The invention relates to the field of microbiological analysis and detection, and particularly discloses a method for detecting clostridium perfringens in drinking natural mineral water. The inspection method comprises the following steps: a, filtering a water sample by adopting a filter membrane, then tightly placing the filter membrane on a bottom culture medium, paving a middle culture medium on the filter membrane, carrying out anaerobic inverted culture for 24-26h at the temperature of 35-37 DEG C, and counting the number of black bacterial colonies; and b, picking 3-5 black bacterial colonies, respectively inoculating the black bacterial colonies on a liquid thioglycolate culture medium, culturing for 18-24 hours at the temperature of 35-37 DEG C, and taking the black bacterial colonies to carry out a confirmation test. By means of the detection method, the black characteristic of the cultured bacterial colony is obvious, identification is easy, and the accuracy of the detection result is high.

Description

Technical field[0001]This application relates to the field of microbiological analysis, and more specifically, it involves a test method of trigminochogenesis in a drinking natural mineral water.Background technique[0002]Gas capsule clostridia (Clostridium Perfringens ) For anaerobic Gram-positive spores, it is widely distributed in the intestinal tract of natural water, soil and human and animal. Gas capsia is a conditional pathogenic bacteria that causes human body to have food poisoning and gas gangrene. According to GB 8537-2018 "Food Safety National Standard Drinking Natural Mine Water", 5 samples were collected from the same batch of mineral water samples, and the sample sample of each sample was 50ml, and the gas capsia is not detected.[0003]At present, the detection method of closenia-gas capsia is usually used in GB 8538-2016 "Food Safety National Standard Drinking Natural Mine Water Test Method", so that the mineral water sample is filtered through the filter membrane, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06C12R1/145
CPCC12Q1/06G01N2333/33
Inventor 郭蕊静赵秉谦高莹柳兰静王仕光李艳蓉
Owner 河北安普检测技术服务有限公司
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