Method for testing clostridium perfringens in drinking natural mineral water
A technology for Clostridium perfringens and inspection methods, which is applied in the directions of microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc. Can not fully guarantee anaerobic conditions, reduce the accuracy of test results and other problems, to achieve the effect of reducing the possibility of false negative results, reducing the possibility and high accuracy
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preparation example
[0038]The following preparation examples, embodiments, and contractions:
[0039]0.22μm filter is purchased from Germany Merck Mid Mingbo, batch number: F8EA35906;
[0040]0.45μm filter was purchased from Germany Merck Mid Mingbo, batch number: F8HA73482;
[0041]TSC Cultivate Substance from Beijing Luqiao Technology Co., Ltd., batch number: 180427;
[0042]Protein Water Posted from Qingdao Hubbo Bio Co., Ltd., Item No .: HB5218-1;
[0043]Truncinsence Caserkin is purchased from Huaan Biotechnology Group in Gansu;
[0044]Polymyxin B Sulfate is purchased from Shenzhen Sakko Biotechnology Co., Ltd., Item No .: 0319-100MU;
[0045]Blade Tianqing, from Beijing Zhongsheng Ruitai Technology Co., Ltd.
preparation example 1
[0047]The SPS medium is prepared by using the following method:
[0048]At a temperature of 100 ° C, 15 g of a trifase, 10 g yeast paste, 0.5 g of ferric ammonium and 15 g agar was dissolved in 1000 mL of distilled water, and the pH was 7.0, and then at a temperature of 121 ° C, the pressure was 103.4. Under the conditions of KPa, high pressure was sterilized for 15 min, then cooled to 50 ° C, 5 ml of a solution of sodium sulfite solution of 100 g / L, 10 ml concentration of 1.2 g / L, a polymyxin B sulfate solution and 10 ml concentration of 12g / L Sulfadiazine sodium aqueous solution is shaken, and the plate is poured.
preparation example 2
[0050]Liquid sulfuric salt (FT) medium is prepared by using the following method:
[0051]At 105 ° C temperature, 15 g of trunca, 0.5 g of L-cystine, 5 g glucose, 5G yeast paste, 2.5 g sodium chloride, 0.5 g of sodium sulfide, 0.001G blade, 0.75 G agar is dissolved in 1000 mL of distilled water, the pH is adjusted to be 7.1, and then at a temperature of 121 ° C, the pressure is 103.4 kPa, high pressure is sterilized for 15 min, cooled to room temperature.
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