46results about How to "Good differential diagnosis" patented technology

The invention discloses a kit for identifying and diagnosing bovine theileria annulata, theileria sergenti and theileria sinensis sp.nov., a preparation method and an application method of the kit. The kit at least comprises an upstream primer of the theileria annulata and a downstream primer of the theileria annulata, an upstream primer of the theileria sergenti and a downstream primer of the theileria sergenti, as well as an upstream primer of the theileria sinensis sp.nov. and a downstream primer of the theileria sinensis sp.nov.

The present disclosure provides methods and compositions that find use in facilitating a diagnosis of inflammatory liver disease in a subject. The methods and compositions generally involve detection of eotaxin-3 (E3) levels, either alone or with levels of eotaxin-1 (E1), and optionally, with levels of CCL22 and, further optionally, with levels of IL15. These levels can be used to facilitate a diagnosis of a liver disease of at least one of autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), and primary sclerosing cholangitis (PSC), and / or to facilitate a differential diagnosis between AIH, PBC, and PSC. The methods and compositions of the present disclosure also find use in facilitating treatment decisions for a subject.

The invention discloses a recombinant expression vector for a lung fluke excretion and secretion protein, an engineering strain, a preparation method and an application thereof. The recombinant expression vector contains the encoding gene of the lung fluke excretion and secretion protein, the nucleotide sequence of the coding gene is shown as SEQ ID No.1, and the expression vector is pQE-80L. Theengineering strain contains the recombinant expression vector, and a host strain is bacillus coli. The method for preparing the lung fluke excretion and secretion protein through the recombinant expression vector comprises four steps of: constructing the engineering strain, culturing, inducting and expressing, and purifying an expression product. The lung fluke excretion and secretion protein canbe used for preparing agents or devices for detecting a paragonimiasis The recombinant expression vector and the engineering strain have the advantages of high expression amount and stable expression, and the lung fluke excretion and secretion protein prepared by the method has high protein yield and high immunoreactivity.

The invention discloses a porcine pseudorabies virus gene deletion attenuated vaccine strain for passage via low temperature of a cell and attenuation via drug screening and an application thereof. The attenuated vaccine strain is prepared through the following steps: based on a porcine pseudorabies virus variant strain (named as strain HeN1, of which the microbial preservation serial No. is CGMCC No. 6656), firstly, carrying out low-temperature passage and screening on a Vero cell to obtain large fragments of deleted viruses including gI, gE, Us9, Us2 and part of inverted repeated sequence which exist in zone US through, and then making the TK gene thereof partially deleted through drug screening. The gene-deleted attenuated vaccine strain is named as strain PRV TP, of which the microbial preservation serial No. is CGMCC No. 12300. A live vaccine or an inactivated vaccine (a single vaccine or combined vaccine) can be prepared from the attenuated vaccine strain disclosed by the invention, and can prevent porcine pseudorabies effectively, and a reagent for diagnosing or treating porcine pseudorabies can be prepared from the attenuated vaccine strain too. According to the porcine pseudorabies virus gene deletion attenuated vaccine strain for passage via low temperature of a cell and attenuation via drug screening and the application thereof, the porcine pseudorabies attenuated vaccine strain PRV TP has the advantages of good safety, efficient protection, convenient differential diagnosis and the like.

The invention discloses a colloidal gold test strip for testing toxoplasma gondii antibodies.The colloidal gold test strip comprises a bottom board, a sample pad, a gold-labeled protein-enveloped conjugate pad, a nitrocellulose membrane containing a detection line T and a quality control line C and an absorbent pad, wherein the sample pad, the conjugate pad, the nitrocellulose membrane and the absorbent pad are disposed on the bottom board.Experiments prove that the colloidal gold test strip is capable of detecting serum antibodies of toxoplasma gondii in animal bodies and achieving differentiating diagnosis of protozoan infection of animals conveniently, cost saving and simple and convenient to operate and is applicable to the animals such as pigs, dogs and cats.

The invention discloses a multi-RPA primer and probe for simultaneously detecting a newcastle disease virus, an infectious bronchitis virus and an H9N2-subtype avian influenza virus. The multi-RPA primer and probe comprise a primer and probe for detecting the newcastle disease virus, a primer and probe for detecting the infectious bronchitis virus and a primer and probe for detecting the H9N2-subtype avian influenza virus. The multi-RPA primer and probe are high in specificity, high in sensitivity and accurate in detection result. The invention further discloses a method for simultaneously detecting the newcastle disease virus, the infectious bronchitis virus and the H9N2-subtype avian influenza virus through multi-RPA. The method comprises the steps of primer synthesis, template RNA extraction, reverse transcription, multi-PCR amplification and amplification product analysis. The detection method is simple in operation and good in stability, and a low-cost, fast and specific field diagnosis method is provided for effective detection and identification of mixed infection of common avian respiratory diseases.

The invention discloses an RPA (recombinase polymerase amplification) primer for detecting a fowl adenovirus serotype 4. The nucleotide sequence of the RPA primer is as shown in SEQ ID No.1 and SEQ ID No.2 in the description. The invention further discloses a method for detecting the RPA of the fowl adenovirus serotype 4, and the method mainly comprises the steps of primer synthesis, extraction of DNA in a sample to be tested, RPA amplification reaction and analysis of an RPA amplification product. According to the RPA primer disclosed by the invention, the specificity is strong, the sensitivity is high, and the detection result is accurate. The detection method disclosed by the invention is simple in operation and good in stability, and a low-cost, rapid and specific on-site diagnosis method for effectively detecting and authenticating chicken cecum hepatitis-hydropericardium syndrome is provided.