Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
46results about How to "Good differential diagnosis" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Porcine pseudorabies virus virulent strain, and gene deletion vaccine strain thereof and applications thereof
ActiveCN102994458AEffective preventionEffective therapeuticMicroorganism based processesAntiviralsRabiesMicrobacterium
The invention discloses a porcine pseudorabies virus virulent strain, and a gene deletion vaccine strain thereof and applications thereof. The porcine pseudorabies virus virulent strain is named as HeN1, the microbial preservation number of the porcine pseudorabies virus virulent strain is CGMCC NO.6656, the deleted gE gene obtaines the gene deletion vaccine strain rPRV-gE-EGFP+ on the basis of the virulent strain HeN1, and the microbial preservation number is CGMCC NO.6657. The virulent strain can be prepared into inactivated vaccine (single vaccine or combined vaccine), the gene deletion vaccine strain rPRV-gE-EGFP+ can be prepared into activated vaccine or inactivated vaccine (single vaccine or combined vaccine) and the like, so that porcine pseudorabies can be effectively prevented or cured, or the gene deletion vaccine strain rPRV-gE-EGFP+ can be prepared into a diagnosis reagent for diagnosing the porcine pseudorabies. The gene deletion vaccine strain rPRV-gE-EGFP+ has the advantages of being good in safety, high in protection efficiency, beneficial to differential diagnosis.
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Differential diagnosis of neurodegeneration
InactiveUS20040014142A1Specific detectionSpecific quantificationDisease diagnosisBiological testingSpecific detectionSynuclein
The present invention relates to new methods for the specific detection, quantification and / or differential diagnosis of neurodegeneration in an individual making use of a combination assay detecting at least three neurological markers in one or more body fluids of said individual, the type and degree of neurodegeneration being reflected in the quantitative changes in the level of all of said neurological markers compared to the control sample. The present invention also relates to methods for the detection of Rab3a, SNAP25 and alpha-synuclein in cerebrospinal fluid and to the use of these methods in a combination assay for specific detection, quantification and / or differential diagnosis of neurodegeneration.
Owner:INNOGENETICS NV
Systems, methods, and diagnostic support tools for facilitating the diagnosis of medical conditions
InactiveUS20190198172A1Readily updatableHigh degreeData processing applicationsHealth-index calculationLaboratory findingMedical treatment
Systems and methods for the accurate and efficient assimilation of clinical and laboratory findings to facilitate a medical diagnostic process are provided. Additionally, such systems and methods may also be employed to facilitate education with respect to specific medical conditions. The systems of the present disclosure comprise a network-based system configured to analyze user input in the form of phenotypic manifestations and, in some cases, pathognomonic data collected from a patient to provide a focused group of possible medical conditions that correlate therewith. Further, in certain embodiments, the systems can automatically generate a list of inquiries, based on the user input and non-discounted medical conditions, to facilitate an efficient inquiry process. Such systems may also be used for educational purpose to facilitate a user's understanding of the pathogenesis and phenotypic manifestations of medical conditions. Methods for using such system for diagnostic and / or educational purposes are also provided.
Owner:NELSON JR ROBERT P
Kit for rapid early-to-mid diagnosis of ovarian cancer and detection method thereof
ActiveCN103954761AGood differential diagnosisHigh sensitivityDisease diagnosisPolyclonal antibodiesTransthyretin
The invention discloses a kit for rapid early-to-mid diagnosis of ovarian cancer. The kit comprises monoclonal antibodies which are respectively prepared from galectin-3, transthyretin, a cancer antigen-125 and human epididymis protein 4; and the kit also comprises a detection antibody, wherein the detection antibody comprises polyclonal antibodies which are respectively prepared from galectin-3, transthyretin, cancer antigen-125 and human epididymis protein 4. The kit has the beneficial effects that the ovarian cancer can be comprehensively detected, benign and malignant pelvic masses before or after menopause can be well judged, gynecological benign ovarian mass and cyst and ovarian cancer can be well identified and judged, the kit has the characteristics of high sensitivity and high accuracy, and the sensitivity and accuracy are above 96 percent.
Owner:GUANGZHOU HENGTAI BIOTECH
RPA primer for detecting chicken infectious laryngotracheitis virus and its detecting method
ActiveCN107287353AStrong specificityHigh sensitivityMicrobiological testing/measurementDNA/RNA fragmentationInfectious laryngotracheitisDiagnosis methods
The invention discloses a RPA primer for detecting chicken infectious laryngotracheitis virus. The nucleotide sequence of the RPA primer is shown as SEQ ID NO.1 and SEQ ID NO.2; the RPA primer is strong in specificity, high in sensitivity, and accurate in detection result. The invention further provides a RPA method for detecting the chicken infectious laryngotracheitis virus. The detecting method is easy to operate, low in equipment requirement, strong in specificity, high in sensitivity, and accurate and reliable in detection result; the detecting method provides a low-cost, fast and specific field diagnosis method for effectively detecting and diagnosing the chicken infectious laryngotracheitis virus.
Owner:HENAN AGRICULTURAL UNIVERSITY
Kit for identifying and diagnosing different species of bovine theileria and method thereof
ActiveCN101570796AReal-time response to infection with parasitesGood differential diagnosisMicrobiological testing/measurementTheileriaMicrobiology
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Monotubular double-chamber syringe needle cylinder and injection method thereof
InactiveCN101502687AReduce usageEasy detectionInfusion syringesIntravenous devicesHuman bodyEngineering
The invention provides a single-cylinder double-chamber injector needle cylinder; the needle cylinder is provided with two pistons which divide the needle cylinder into a front chamber and a back chamber; the two pistons are connected with each other by a flexible connecting piece; the front chamber piston is provided with a through hole and a sealing device, thus realizing double drug administration of CT and MR contrast agent and physiological saline; remaining contrast agent in the cylinder is injected into human body by a physiological saline washing pipe and enters vein after being driven, so that the contrast agent reaches the target organ rapidly, thus increasing contrast of pathological changes with surrounding tissues and improving checkout of the pathological changes; meanwhile, blood supply information of nidus is increased, which facilitates diagnosis and differential diagnosis and increases certainty factor of diagnosis by the doctors; when CT and MR angiogram is carried out, contrast between the blood vessel and surrounding tissues is increased, which improves the angiogram effect; perfusion imaging is carried out on CT and MR, so that the contrast agent which enters the targeted organ has more constant rate and more accurate data is obtained; on the basis, more abundant information is expected to be obtained with the same amount of contrast agent used or the use amount of the contrast agent is expected to be reduced.
Owner:龚静山
Primer group for identifying porcine circovirus types 1, 2 and 3 and application of primer group
InactiveCN109762943ACondition optimization is simpleEasy to observe the resultsMicrobiological testing/measurementDNA/RNA fragmentationPorcine circovirus type 1Genotype
The invention provides a primer group for identifying porcine circovirus types 1, 2 and 3 and application of the primer group. The sequences of the primer group are shown in SEQ ID NO.1 -4. On the basis of the genome structure characteristics of the three genotypes (PCV1, PCV2 and PCV3) of porcine circovirus, genome specific conserved areas of the three genotypes (PCV1, PCV2 and PCV3) of porcine circovirus are selected as upstream primer design areas, and downstream primers are designed separately in differential areas of genomes generated according to the three genotypes (PCV1, PCV2 and PCV3). The primer is simple in condition optimization, convenient and high in reaction speed, and a result is observed conveniently; an assembled kit can be used for rapid differential diagnosis of the three genotypes of porcine circovirus (PCV1, PCV2 and PCV3).
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
Preparation method of kit for early-to-mid rapid diagnosis of ovarian cancer
The invention discloses a preparation method of a kit for early-to-mid rapid diagnosis of an ovarian cancer. The method comprises the following steps: 1. preparation of antigens: required antigens are prepared by adopting genes of galectin-3, transthyretin, carcinoma antigen-125 and human epididymis protein 4; 2, preparation of capture antibodies: the antigens are used for immunization of a mammal so as to obtain corresponding monoclonal antibodies; 3, preparation of detection antibodies: the antigens are used for immunization of the mammal so as to obtain corresponding polyclonal antibodies; 4, coating of the capture antibodies; 5, sealing and sample adding. The method has the benefits as follows: the ovarian cancer can be detected in the most comprehensive manner, the benign and malignant of a pelvic lump before menopause or after menopause can be better judged, differential diagnosis can be better performed on gynecology benign ovary lump and cyst as well as the ovarian cancer, the method has the characteristics of high sensitivity and high accuracy, and the sensitivity and accuracy can reach over 96 percent.
Owner:GUANGZHOU HENGTAI BIOTECH
Colloidal gold test strip for testing toxoplasma gondii antibodies
InactiveCN105759030AStrong characteristicIncreased sensitivityBiological material analysisBiological testingBiologyCost savings
The invention discloses a colloidal gold test strip for testing toxoplasma gondii antibodies.The colloidal gold test strip comprises a bottom board, a sample pad, a gold-labeled protein-enveloped conjugate pad, a nitrocellulose membrane containing a detection line T and a quality control line C and an absorbent pad, wherein the sample pad, the conjugate pad, the nitrocellulose membrane and the absorbent pad are disposed on the bottom board.Experiments prove that the colloidal gold test strip is capable of detecting serum antibodies of toxoplasma gondii in animal bodies and achieving differentiating diagnosis of protozoan infection of animals conveniently, cost saving and simple and convenient to operate and is applicable to the animals such as pigs, dogs and cats.
Owner:CHINA AGRI UNIV
Kit, primer pair, probe and method for detecting vibrio parahaemolyticus
InactiveCN109913565AStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesNucleotideVibrio parahemolyticus
The invention discloses a kit, a primer pair, a probe and a method for detecting vibrio parahaemolyticus. The kit comprises an RPA reaction system which comprises an RPA primer probe mixture, the RPAprimer probe mixture comprises the primer pair and the probe, a nucleotide sequence of an upstream primer in the primer pair is shown as SEQ ID NO.1 while that of a downstream primer in the primer pair is shown as SEQ ID NO.2, and nucleotide sequences of the probe are shown as SEQ ID NO.3 and SEQ ID NO.4. The primer is high in specificity and sensitivity and accurate in detection result. By combining RPA technology for detecting vibrio parahaemolyticus with a colloidal gold flow measuring chromatography test paper strip, quick detection of vibrio parahaemolyticus can be realized, and 10pg of vibrio parahaemolyticus DNA can be detected at least.
Owner:SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI +1
Chemiluminescence kit for rapid diagnosis of uterine sarcoma in early and middle stages
InactiveCN107677826AAccurate detectionGood differential diagnosisMaterial analysisDeath ReceptorsWilms' tumor
The invention discloses a chemiluminescence kit for rapid diagnosis of uterine sarcoma in early and middle stages. Tumor markers comprise CD10, a death receptor and a growth differentiation factor-15.The kit comprises a tumor marker standard, a tumor marker monoclonal capture antibody-coated microwell plate, a horseradish peroxidase or alkaline phosphatase-labeled tumor marker monoclonal detection antibody, a chemiluminescent substrate solution and a concentrated scrubbing solution. The chemiluminescence kit has high sensitivity of 70% or more and strong accuracy of 70% or more, is low in cost and reduces operation steps and detection error.
Owner:GUANGZHOU HENGTAI BIOTECH
Methods and compositions for diagnosis of inflammatory liver disease
ActiveUS9535071B2Good differential diagnosisDiagnosis can be complexHealth-index calculationDigestive systemPrimary biliary cirrhosisInflammatory bowel disease
The present disclosure provides methods and compositions that find use in facilitating a diagnosis of inflammatory liver disease in a subject. The methods and compositions generally involve detection of eotaxin-3 (E3) levels, either alone or with levels of eotaxin-1 (E1), and optionally, with levels of CCL22 and, further optionally, with levels of IL15. These levels can be used to facilitate a diagnosis of a liver disease of at least one of autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), and primary sclerosing cholangitis (PSC), and / or to facilitate a differential diagnosis between AIH, PBC, and PSC. The methods and compositions of the present disclosure also find use in facilitating treatment decisions for a subject.
Owner:MEDIZINISCHE HOCHSCHULE HANNOVER +1
In vitro diagnostic method for patients with splenic marginal zone lymphoma
InactiveUS20130267432A1Good differential diagnosisNucleotide librariesMicrobiological testing/measurementNucleotideMarginal zone lymphoma
The present invention refers to an in vitro SMZL diagnostic method based on analysis of the expression of a series of genes located in a very specific deleted region of human chromosome 7. The deleted region in SMZL patients has been defined by means of high-resolution array CGH and is located at 7q22.1 between bases 99925039-101754718, preferably between 99925039-101348479, and more preferably between 100260234-100596921 and / or 101244026-101754718, which is the location for the following genes: CUX1, SH2B2, EPHB4, SLC12A9, TRIP6, SRRT, ACHE, UFSP1, TRIM56, SERPINE1, APIS1, VGF, TSC22D4, HRBL, LRCH4, MUC3, MUC12 and MUC17. The present invention further describes in vitro SMZL diagnostic kits that comprise at least one probe that recognizes the deleted region at 7q22.1 or hybridizes with at least one of the nucleotide sequences of the genes mentioned above.
Owner:FUNDACION DE INVESTIGACION DEL CANCER DE LA UNIV DE SALAMANCA FICUS
Kit for rapidly detecting natural infection antibodies of animal hydatid
InactiveCN110058010AAvoid interferenceSolving the Biggest Dilemma in Differential DiagnosisBiological testingTest stripsIndividual animal
The invention relates to the field of immunocolloidal gold chromatography analysis, in particular to a kit for rapidly detecting natural infection antibodies of animal hydatid. The kit can specifically detect the natural infection antibodies of the hydatid, and has no cross reaction with the vaccine immune antibody. The kit comprises a colloidal gold test paper card and a sample diluent, wherein the colloidal gold test paper card comprises a closed box body and a reaction test strip tiling in the box body, the box body is respectively provided with an observation window and a sample adding hole connected to the reaction test strip; and the sample diluent is a mixed solution including 0.01M PH7.2 PBS and 1% tween-80. The invention provides a simple, rapid and scientific method for diagnosing echinococcosis infection, which can quickly detect whether a blood sample contains natural infection antibodies of animal hydatid.
Owner:SHENZHEN ZHENRUI BIOTECH CO LTD
Rapid diagnostic kit for uterine sarcoma in early and middle stages and preparation method of kit
InactiveCN105842461AAccurate detectionGood differential diagnosisBiological material analysisBiological testingPelvic lumpMalignancy
The invention discloses a rapid diagnostic kit for a uterine sarcoma in early and middle stages .The kit is prepared from CD10, death reporters and growth differentiation factors-15 .The invention further discloses a preparation method of the rapid diagnostic kit for the uterine sarcoma in the early and middle stages .By means of the rapid diagnostic kit for the uterine sarcoma in the early and middle stages, the uterine sarcoma can be detected more accurately and comprehensively, benign and malignant pelvic masses can be better judged, differential diagnosis is better conducted on gynecological benign uterine masses and uterine sarcomas, and the sensitivity and the accuracy of the kit both reach 70%.
Owner:GUANGZHOU HENGTAI BIOTECH
Investigation and analysis method of thyroid function abnormalities of healthy people in water-source-originated high-iodine region
InactiveCN106934751AExpedited screeningEasy to monitorData processing applicationsDisease diagnosisDiseaseStatistical analysis
The invention discloses an investigation and analysis method for abnormal thyroid function of healthy people in water-sourced high-iodine areas. 3218 healthy people were taken for physical examination, and fasting venous blood was collected to separate serum, and serum thyroid-stimulating hormone (TSH) was measured with an immunochemiluminescent instrument. , total triiodothyronine (TT3), total thyroxine (TT4), free triiodothyronine (FT3), and free thyroxine (FT4), and statistically analyzed the test results. The detection rate of thyroid disease is affected by many factors such as gender, age, race, and region. In areas with high iodine in water sources, the detection rate of abnormal thyroid function in healthy people increases year by year, mainly subclinical hypothyroidism, and is related to gender and Age-related, especially the higher incidence of subclinical hypothyroidism. Through the method of the invention, the thyroid function in an area can be screened and analyzed conveniently, the monitoring of thyroid diseases is strengthened, the early diagnosis of thyroid diseases is beneficial, and the health of the people is ensured.
Owner:CANGZHOU MEDICAL COLLEGE
Mono-cloning antibody of anti-human cancer protein iASPP and use thereof
InactiveCN1854156AConducive to further research and elucidation of pathogenesisStudy and elucidate pathogenesisImmunoglobulins against animals/humansVector-based foreign material introductionHuman cancerAntigen
Owner:INST OF HEMATOLOGY & BLOOD HOSPITAL CHINESE ACAD OF MEDICAL SCI
Application of Mycobacterium tuberculosis protein in preparation of products to diagnose patients with latent tuberculosis infection and/or active tuberculosis
ActiveCN108267589AGood differential diagnosisStrong specificityBiological testingImmunoassaysParatuberculosisMedicine
The invention provides application of Mycobacterium tuberculosis protein in the preparation of products to diagnose patients with latent tuberculosis infection and / or active tuberculosis. A method provided herein can detect the levels of three different Mycobacterium tuberculosis protein serum antibodies at the same time, thereby better identifying and diagnosing patients with latent tuberculosisinfection and patients with active tuberculosis; the sensitivity is higher, and the specificity is better.
Owner:BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
Recombinant expression vector of excretion exudation protein of lung fluke, engineering bacterium, preparation method and uses thereof
InactiveCN101319224BHigh yieldEnhance immune responseBacteriaMicroorganism based processesBiotechnologyEscherichia coli
The invention discloses a recombinant expression vector for a lung fluke excretion and secretion protein, an engineering strain, a preparation method and an application thereof. The recombinant expression vector contains the encoding gene of the lung fluke excretion and secretion protein, the nucleotide sequence of the coding gene is shown as SEQ ID No.1, and the expression vector is pQE-80L. Theengineering strain contains the recombinant expression vector, and a host strain is bacillus coli. The method for preparing the lung fluke excretion and secretion protein through the recombinant expression vector comprises four steps of: constructing the engineering strain, culturing, inducting and expressing, and purifying an expression product. The lung fluke excretion and secretion protein canbe used for preparing agents or devices for detecting a paragonimiasis The recombinant expression vector and the engineering strain have the advantages of high expression amount and stable expression, and the lung fluke excretion and secretion protein prepared by the method has high protein yield and high immunoreactivity.
Owner:ARMY MEDICAL UNIV
Collection of probes for autistic spectrum disorders and their use
InactiveUS20160017424A1Facilitate primaryFacilitate differential diagnosisNucleotide librariesMicrobiological testing/measurementAutism spectrum disorderBiomarker (petroleum)
The present invention relates to collections of probes or their complements that recognize a set of biomarkers related to autism spectrum disorders (ASDs), as well as to methods utilizing these probes for diagnosing whether a subject has an ASD or has a predisposition for developing an ASD.
Owner:THE RES FOUND OF STATE UNIV OF NEW YORK +2
Immunoassay kit for detecting M-type phospholipase A2 receptor-IgG as well as preparation method and detection method of immunoassay kit
PendingCN112147339AAccurate differential diagnosisGood differential diagnosisDisease diagnosisBiological testingImmune profilingReagent strip
The invention discloses an immunoassay kit for detecting M-type phospholipase A2 receptor-IgG as well as a preparation method and detection method of the immunoassay kit, and belongs to the field of immunoassay technologies and nano biotechnologies. The kit comprises a reaction buffer, a cleaning solution, an enhancement solution, a PLA2R-coated magnetic particle solution, an europium-labeled goatanti-human IgG antibody solution which are integrated on a reagent strip and is additionally provided with two bottles of freeze-dried calibrators of anti-PLA2R-IgG. The kit can provide a reaction system close to a homogeneous phase, the content of anti-PLA2R-IgG is detected, the detection time is shortened, the efficiency is improved, and convenience is provided for clinical use.
Owner:浙江博实生物科技有限公司
A Porcine Pseudorabies Virus Gene Deletion Attenuated Vaccine Strain and Its Application
ActiveCN106282128BImprove securityImprove protection efficiencyViral antigen ingredientsMicroorganism based processesLarge fragmentVariant strain
Owner:HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Application of mycobacterium tuberculosis protein in preparation of product for diagnosing latent tuberculosis infectors and/or active pulmonary tuberculosis
ActiveCN111521819AGood differential diagnosisStrong specificityBiological testingImmunoassaysTuberculosis mycobacteriumLatent tuberculosis
The invention provides application of mycobacterium tuberculosis protein in preparation of products for diagnosing latent tuberculosis infectors and active pulmonary tuberculosis. The method providedby the invention can be used for detecting the levels of three different mycobacterium tuberculosis protein serum antibody responses at the same time, so that latent tuberculosis infectors and activetuberculosis patients can be better identified and diagnosed, the sensitivity is higher, and the specificity is better.
Owner:BEIJING CHEST HOSPITAL CAPITAL MEDICAL UNIV
A colloidal gold test strip for detecting toxoplasma gondii antibody
InactiveCN105759030BStrong characteristicIncreased sensitivityBiological material analysisBiological testingBiologyCost savings
Owner:CHINA AGRI UNIV
Recombinant expression vector of excretion exudation protein of lung fluke, engineering bacterium, preparation method and uses thereof
InactiveCN101319224AHigh yieldEnhance immune responseBacteriaMicroorganism based processesNucleotideBacillus coli
The invention discloses a recombinant expression vector for a lung fluke excretion and secretion protein, an engineering strain, a preparation method and an application thereof. The recombinant expression vector contains the encoding gene of the lung fluke excretion and secretion protein, the nucleotide sequence of the coding gene is shown as SEQ ID No.1, and the expression vector is pQE-80L. The engineering strain contains the recombinant expression vector, and a host strain is bacillus coli. The method for preparing the lung fluke excretion and secretion protein through the recombinant expression vector comprises four steps of: constructing the engineering strain, culturing, inducting and expressing, and purifying an expression product. The lung fluke excretion and secretion protein can be used for preparing agents or devices for detecting a paragonimiasis The recombinant expression vector and the engineering strain have the advantages of high expression amount and stable expression, and the lung fluke excretion and secretion protein prepared by the method has high protein yield and high immunoreactivity.
Owner:ARMY MEDICAL UNIV
A kind of multiple rpa primers and probes for simultaneous detection of ndv, ibv and h9n2 subtype aiv and its detection method
ActiveCN107287349BStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesDiseaseRNA extraction
The invention discloses a multi-RPA primer and probe for simultaneously detecting a newcastle disease virus, an infectious bronchitis virus and an H9N2-subtype avian influenza virus. The multi-RPA primer and probe comprise a primer and probe for detecting the newcastle disease virus, a primer and probe for detecting the infectious bronchitis virus and a primer and probe for detecting the H9N2-subtype avian influenza virus. The multi-RPA primer and probe are high in specificity, high in sensitivity and accurate in detection result. The invention further discloses a method for simultaneously detecting the newcastle disease virus, the infectious bronchitis virus and the H9N2-subtype avian influenza virus through multi-RPA. The method comprises the steps of primer synthesis, template RNA extraction, reverse transcription, multi-PCR amplification and amplification product analysis. The detection method is simple in operation and good in stability, and a low-cost, fast and specific field diagnosis method is provided for effective detection and identification of mixed infection of common avian respiratory diseases.
Owner:HENAN AGRICULTURAL UNIVERSITY
Kit for rapidly detecting animal hydatid natural infection antibody
PendingCN111257571AAvoid interferenceRapid differential diagnosisBiological material analysisBiological testingEnzyme bindingA-DNA
The invention discloses a kit for rapidly detecting an animal hydatid natural infection antibody. The kit comprises the following raw materials in parts by weight: a reagent A including 20-30 mmol / L of a Hyd diluent, 90-110 mmol / L of a buffer solution, 0.03-0.05 mmol / L of a DNA sequence solution and 0.2-0.4 mmol / L of a developing solution A; a reagent B including 20 to 30mmol / L of Hyd diluent, 0.5to 0.7 mmol / L of Hyd enzyme conjugate, 90 to 110mmol / L of buffer solution and 0.1 to 0.3 mmol / L of developing solution B. The invention relates to the technical field of the detection of the naturalinfection antibody of the hydatid. The invention discloses the kit for rapidly detecting the animal hydatid natural infection antibody, the DNA sequence solution in the reagent A and a vaccine immuneantibody are used for recognition, the color development is carried out by using the color development solution A; then, the Hyd enzyme conjugate in the reagent B is used for being specifically combined with the hydatid natural infection antibody; the development solution B is matched for development such that the antibody generated by natural infection can be selectively detected, the interference of the vaccine immune antibody is avoided, the infection of hydatid disease can be quickly, simply, conveniently and accurately identified and diagnosed, and the problem that the antibody detectionreagent cannot distinguish the vaccine immune antibody from the antibody generated by natural infection is effectively avoided.
Owner:NANJING YIKE POPULATION HEALTH RES INST CO LTD
A kit, primer pair, probe and method for detecting Vibrio parahaemolyticus
InactiveCN109913565BStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesNucleotideVibrio parahemolyticus
The invention discloses a kit, a primer pair, a probe and a method for detecting vibrio parahaemolyticus. The kit comprises an RPA reaction system which comprises an RPA primer probe mixture, the RPAprimer probe mixture comprises the primer pair and the probe, a nucleotide sequence of an upstream primer in the primer pair is shown as SEQ ID NO.1 while that of a downstream primer in the primer pair is shown as SEQ ID NO.2, and nucleotide sequences of the probe are shown as SEQ ID NO.3 and SEQ ID NO.4. The primer is high in specificity and sensitivity and accurate in detection result. By combining RPA technology for detecting vibrio parahaemolyticus with a colloidal gold flow measuring chromatography test paper strip, quick detection of vibrio parahaemolyticus can be realized, and 10pg of vibrio parahaemolyticus DNA can be detected at least.
Owner:SHANGHAI VETERINARY RES INST CHINESE ACAD OF AGRI SCI +1
A rpa primer for detecting poultry adenovirus serotype 4 and its detection method
ActiveCN107385110BStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesSerum igeDiagnosis methods
The invention discloses an RPA (recombinase polymerase amplification) primer for detecting a fowl adenovirus serotype 4. The nucleotide sequence of the RPA primer is as shown in SEQ ID No.1 and SEQ ID No.2 in the description. The invention further discloses a method for detecting the RPA of the fowl adenovirus serotype 4, and the method mainly comprises the steps of primer synthesis, extraction of DNA in a sample to be tested, RPA amplification reaction and analysis of an RPA amplification product. According to the RPA primer disclosed by the invention, the specificity is strong, the sensitivity is high, and the detection result is accurate. The detection method disclosed by the invention is simple in operation and good in stability, and a low-cost, rapid and specific on-site diagnosis method for effectively detecting and authenticating chicken cecum hepatitis-hydropericardium syndrome is provided.
Owner:HENAN AGRICULTURAL UNIVERSITY
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com