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Rapid diagnostic kit for uterine sarcoma in early and middle stages and preparation method of kit

A technology for rapid diagnosis of uterine sarcoma, applied in biological testing, material inspection products, measuring devices, etc., can solve the problems of late diagnosis, high cost of pathological diagnosis and detection, and influence on pathological sections, and achieve low production cost, good judgment, and good Effect of Differential Diagnosis

Inactive Publication Date: 2016-08-10
GUANGZHOU HENGTAI BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Postoperative histopathological examination is a kind of sampling examination, which has requirements on the quality of specimens, which directly affects the quality of pathological sections; pathological diagnosis depends on the experience and professional knowledge of the diagnostician, and has a certain degree of subjectivity; Costly and may be diagnosed too late

Method used

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  • Rapid diagnostic kit for uterine sarcoma in early and middle stages and preparation method of kit
  • Rapid diagnostic kit for uterine sarcoma in early and middle stages and preparation method of kit
  • Rapid diagnostic kit for uterine sarcoma in early and middle stages and preparation method of kit

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Embodiment Construction

[0063] The early and mid-term rapid diagnosis kit for uterine sarcoma of the present invention will be described in detail below in conjunction with the accompanying drawings and specific examples.

[0064] Early and mid-term rapid diagnosis kit for uterine sarcoma, including capture antibody, capture antibody blocking buffer, standard, labeled antibody, washing solution and chromogenic solution, etc. The labeled antibody is selected HRP marker Secondary Antibodies . The capture antibodies include CD10, death receptor 6 and growth differentiation factor-15 respectively prepared monoclonal antibodies; through the joint rapid detection of CD10, death receptor 6 and growth differentiation factor-15, the detection accuracy can be effectively improved It effectively overcomes the shortcomings caused by the detection of a certain tumor marker alone, and its sensitivity and accuracy both reach more than 70%.

[0065] The capture antibodies are CD10, death receptor 6 and growth d...

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Abstract

The invention discloses a rapid diagnostic kit for a uterine sarcoma in early and middle stages .The kit is prepared from CD10, death reporters and growth differentiation factors-15 .The invention further discloses a preparation method of the rapid diagnostic kit for the uterine sarcoma in the early and middle stages .By means of the rapid diagnostic kit for the uterine sarcoma in the early and middle stages, the uterine sarcoma can be detected more accurately and comprehensively, benign and malignant pelvic masses can be better judged, differential diagnosis is better conducted on gynecological benign uterine masses and uterine sarcomas, and the sensitivity and the accuracy of the kit both reach 70%.

Description

Technical field: [0001] The invention relates to the field of kits for diagnosis and detection, in particular to a kit for early and mid-stage rapid diagnosis of uterine sarcoma and a preparation method thereof. Background technique: [0002] Uterine sarcoma is a type of mesenchymal reproductive tract malignancy originating from the mixed components of uterine smooth muscle, endometrial stroma (or epithelium) and non-epithelium. Due to the lack of specific clinical manifestations, preoperative diagnosis is difficult. The most common signs are enlarged uterus, most of which are obviously enlarged, and uterine masses increase rapidly in a short period of time, pelvic and abdominal masses, or ascites, abdominal pain, and low back pain ; The surface of the mass is uneven or nodular. Symptoms of uterine enlargement are often misdiagnosed as "uterine fibroids" before surgery, and the misdiagnosis rate is as high as 50%. Many patients therefore receive inappropriate surgical treat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57442G01N33/57484G01N33/6863G01N33/6872G01N2333/475G01N2333/705G01N2333/70596
Inventor 余跃飞
Owner GUANGZHOU HENGTAI BIOTECH
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