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Chemiluminescence kit for rapid diagnosis of uterine sarcoma in early and middle stages

A chemiluminescent reagent and rapid diagnosis technology, which is applied in scientific instruments, measuring devices, instruments, etc., can solve problems affecting early detection and treatment of uterine sarcoma, high cost of pathological diagnosis and detection, and affecting pathological slices, etc., to achieve novel design and good quality. Judgment, good differential diagnosis effect

Inactive Publication Date: 2018-02-09
GUANGZHOU HENGTAI BIOTECH
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  • Claims
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Problems solved by technology

Postoperative histopathological examination is a kind of sampling examination, which has requirements on the quality of specimens, which directly affects the quality of pathological sections; pathological diagnosis depends on the experience and professional knowledge of the diagnostician, and has a certain degree of subjectivity; Costly and may be diagnosed too late
[0008] At present, there is no rapid and efficient diagnostic kit for uterine sarcoma on the market, which seriously affects the early detection and treatment of uterine sarcoma

Method used

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  • Chemiluminescence kit for rapid diagnosis of uterine sarcoma in early and middle stages
  • Chemiluminescence kit for rapid diagnosis of uterine sarcoma in early and middle stages
  • Chemiluminescence kit for rapid diagnosis of uterine sarcoma in early and middle stages

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Embodiment Construction

[0037] The chemiluminescence kit for rapid diagnosis of uterine sarcoma in the early and mid-term of the present invention will be described in detail below with reference to the accompanying drawings and specific examples.

[0038] Chemiluminescence kit for rapid diagnosis of uterine sarcoma in the early and mid-term, including capture antibody, capture antibody blocking buffer, standard, HRP-labeled detection antibody, washing solution and color developing solution, etc. The tumor marker components are: CD10, death receptor and growth differentiation factor-15. The capture antibody includes monoclonal antibodies prepared separately from 3 kinds of markers; the kit includes a tumor marker standard, a microplate coated with a tumor marker monoclonal capture antibody, horseradish peroxidase or alkaline phosphate Enzyme-labeled tumor marker monoclonal detection antibody, chemiluminescence substrate solution, concentrated washing solution. The horseradish peroxidase chemiluminescen...

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Abstract

The invention discloses a chemiluminescence kit for rapid diagnosis of uterine sarcoma in early and middle stages. Tumor markers comprise CD10, a death receptor and a growth differentiation factor-15.The kit comprises a tumor marker standard, a tumor marker monoclonal capture antibody-coated microwell plate, a horseradish peroxidase or alkaline phosphatase-labeled tumor marker monoclonal detection antibody, a chemiluminescent substrate solution and a concentrated scrubbing solution. The chemiluminescence kit has high sensitivity of 70% or more and strong accuracy of 70% or more, is low in cost and reduces operation steps and detection error.

Description

Technical field: [0001] The invention relates to the field of kits for cancer diagnosis and detection, in particular to a chemiluminescence kit for rapid diagnosis of uterine sarcoma in the early and mid-term. Background technique: [0002] Uterine sarcoma is a type of mesenchymal reproductive tract malignant tumor originating from uterine smooth muscle, endometrial stroma (or epithelium) and non-epithelial mixed components. The tissue composition is complex, and the clinical manifestations are similar to uterine fibroids but lack specificity. Due to the lack of specific clinical manifestations, preoperative diagnosis is difficult. The most common signs are enlarged uterus, and most of them are enlarged. In a short period of time, uterine masses increase rapidly, pelvic and abdominal masses, or ascites, abdominal pain and low back pain ; The surface of the mass is uneven or nodular. Uterine enlargement symptoms are often misdiagnosed as "uterine fibroids" before surgery, and the...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574G01N33/535
CPCG01N33/577G01N33/535G01N33/57442G01N33/57484
Inventor 余跃飞
Owner GUANGZHOU HENGTAI BIOTECH
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