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Kit for identifying and diagnosing different species of bovine theileria and method thereof

A technology for differential diagnosis and kits, applied in biochemical equipment and methods, microbial determination/inspection, etc., and can solve problems such as individual inspections, application limitations, poor specificity, etc.

Active Publication Date: 2009-11-04
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, its use is limited when conducting large-scale censuses in areas infested by multiple species of Theileria due to cross-reaction problems between some Theileria species
Enzyme-linked immunosorbent assay (ELISA) serological tests are increasingly used to detect parasite-specific antibodies, but this method can only detect a specific species of worms alone, mainly due to the various ELISAs for detection of Theileria antibodies There is poor specificity, and ELISA, as a serological detection method, sometimes only shows whether the tested animals have been infected with pathogens

Method used

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  • Kit for identifying and diagnosing different species of bovine theileria and method thereof
  • Kit for identifying and diagnosing different species of bovine theileria and method thereof
  • Kit for identifying and diagnosing different species of bovine theileria and method thereof

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Embodiment Construction

[0024] A specific example of the present invention is provided below, and the reagent used in this example includes:

[0025] (1) Reagents used to extract genome from blood:

[0026] a. Red blood cell lysate (Gentra company)

[0027] b. cell lysate (Gentra company)

[0028] c. Protein precipitation agent (Gentra company)

[0029] d. DNA hydration agent (Gentra company)

[0030] e. Isopropanol (commercially available)

[0031] f.70% ethanol (commercially available)

[0032] (2) Reagents used in PCR reaction:

[0033] a.5×Buffer buffer (TakaRa company)

[0034] b. 2.5mM dNTP mixture (TakaRa company)

[0035] c.rTaq enzyme (TakaRa company)

[0036] d. Genomic DNA

[0037] e. 50pM primer

[0038] f. Sterilized water deionized water

[0039] Each primer used in the present invention is respectively:

[0040] b. Upstream primers for Theileria annuli:

[0041] AnS 5'-GAGTTCAAGGACTAGAACC-3'

[0042] b. Downstream primers for Theileria annuli:

[0043] AnR 5'-GAGTCTACTGG...

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Abstract

The invention discloses a kit for identifying and diagnosing bovine theileria annulata, theileria sergenti and theileria sinensis sp.nov., a preparation method and an application method of the kit. The kit at least comprises an upstream primer of the theileria annulata and a downstream primer of the theileria annulata, an upstream primer of the theileria sergenti and a downstream primer of the theileria sergenti, as well as an upstream primer of the theileria sinensis sp.nov. and a downstream primer of the theileria sinensis sp.nov.

Description

technical field [0001] The invention relates to a kit capable of differentially diagnosing Theileria annuli, Theileria cercei and Theileria sinensis, and a preparation and use method of the kit. Background technique [0002] Bovine Theileriasis is a tick-borne disease of cattle. There are 6 types of Theileria infecting cattle, of which there are two that are the most pathogenic and cause serious economic problems, one is small Theileria and the other is Ring Theileria insect. Theileria bovis that exist in China mainly include Theileria annulus, Theileria thieri, Theileria buffalo / Theileria orientalis and Theileria sinensis. Theileria sinensis is a new species of Theileria bovis transmitted by blood ticks in Qinghai discovered by Bai Qi et al. It is mainly distributed in Gansu and Qinghai provinces in China. The main vector of Theileria bovine, the bovine tick, is widely present in tropical and subtropical countries. In view of the serious harm of this type of disease, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 罗建勋殷宏刘军龙关贵全李有全刘志杰刘爱红马米玲
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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