The invention discloses a colorful rubber floor mat and a manufacturing method of the colorful rubber floor mat. The manufacturing method of the colorful rubber floor mat includes the steps that waste rubber is smashed to serve as bottom-layer particles, then the smashed waste rubber and adhesives are evenly mixed, and a molded bottom layer is formed in a heated and pressurized mode; raw materials including ethylene-propylene-diene monomer, rubber oil, toner, white carbon black, ultraviolet-resistant agents, accelerants, zinc oxide, antioxidants, calcium carbonate and the like are evenly mixed, banburying and open milling are sequentially carried out, rubber is cut into pieces, the rubber pieces are cooled and smashed to serve as face-layer particles, then the smashed rubber pieces and adhesives are evenly mixed, and a molded face layer is formed in a heated and pressurized mode; finally the bottom layer and the face layer are bonded with the hot mold technology. The service life of the colorful rubber floor mat manufactured with the manufacturing method is as long as 5 years-8 years; the colorful rubber floor mat can be resistant to the 180-DEG C high temperature; the buffering performance is good; a slightly-protruding particle feeling is achieved on the surface, a special soft feeling is achieved during treading, and the colorful rubber floor mat has the elasticity and is resistant to sliding; no harmful matter is contained; the colorful rubber floor mat is basically free of influences of expansion caused by heat and contraction caused by cold, and is safe, comfortable, durable and attractive in appearance.