35results about How to "Best extraction conditions" patented technology

The invention discloses an extraction and purification technology of four kinds of flavone C-glycosides in moso bamboo leaves. The active ingredients of moso bamboo leaves are extracted by a heat reflux extraction process, and the best extraction technology for obtaining four kinds of flavone C-glycosides through a single-factor experiment and an orthogonal experiment comprises the following steps: extracting with 75% ethanol for 2h at 70 DEG C according to a solid-liquid ratio of 1:20, and repeatedly extracting for 4 times; purifying the crude extract of flavone by use of macroporous resin, wherein D101 macroporous resin is the best choice according to comparison of static saturated adsorption capacity and desorption capacity; and further purifying the component by use of polyamide resin, wherein the particle size of the polyamide resin is 0.15-0.25mm according to the comparison of the dynamic adsorption screening results of three common kinds of polyamide resin, and a 40% ethanol solution is adopted for purifying the four kinds of flavone C-glycosides. Through the treatment, the final purity of the four kinds of flavone C-glycosides bamboo leaf flavone powder reaches 76.939mg.g<-1>.

The invention provides a method for extracting alfalfa polysaccharide by use of complex enzyme (cellulase, papain and pectinase). The extracting temperature of the alfalfa polysaccharide is reduced, the extracting rate of the alfalfa polysaccharide is improved, and the method is suitable for popularization. The method comprises the following steps: determining the test range of factors influencing the extracting rate of the alfalfa polysaccharide through a single-factor test, determining the optimum proportion of the complex enzyme through an orthogonal test, then obtaining the relation between a response value and an influence factor by combing a central complex test with the response surface methodology (RSM), and computing the level of the influence factor when the maximum response value is obtained so as to determine the optimum extracting condition of extracting the alfalfa polysaccharide by complex enzyme, wherein the cellulase is 2.5%, the papain is 2.0%, pectinase is 3.0%, the extracting temperature is 52.7 DEG C, the extracting pH value is 3.87, the extracting solid-to-liquid ratio is 78.91, and the extracting time is 2.73 hours. The extracting rate of alfalfa polysaccharide is more than 5.0% under the condition provided by the invention.

The invention provides a method for extracting selenium protein from selenium-enriched green tea, which comprises the following steps: extracting selenium-enriched green tea powder by 40 percent of ethanol for three times repeatedly; removing tea polyphenols to obtain selenium-enriched green tea leaves; extracting the selenium-enriched green tea leaves with high protein and selenium for 16 hours at the temperature of 40 DEG by 0.1 M of NaOH solution, the ratio of liquid to material of the NaOH solution is 1:30, which are the best conditions for extracting the selenium protein. Selenium protein extractive in the selenium-enriched green tea selenium protein extracted by the method at least contains 17 kinds of amino acids, and furthermore the selenium protein extractive contains rich essential amino acids.

The invention discloses a response surface method optimized method for extracting 1-deoxynojirimycin (1-DNJ) in mulberry leaves with an acidic ethanol solution. The method is characterized in that the acidic ethanol solution is taken as the extraction solvent for extracting the 1-DNJ in the mulberry leaves, and the extraction condition is optimized by the response surface method so that the optimal condition is obtained, namely, the solution-to-material ratio is 25, and in other words, 25mL of extraction solvent is used for each gram of crude mulberry leaf powder; the method comprises the steps of extracting for 2.7h under the condition of 80 DEG C, re-extracting once, purifying the filtrate, and then concentrating the filtrate to obtain the 1-DNJ extract; the method is capable of obtaining about 0.2% of mulberry leaf 1-DNJ extraction rate, which is remarkably higher than the traditional ethanol extraction rate 0.00139% and the diluted acid extraction efficiency 0.00147%; obviously, the diluted acid and the ethanol have synergistic effect in the mulberry leaf 1-DNH extraction process and unexpected effect is generated, and therefore, theoretical support and technical guidance are provided for development and utilization of the mulberry leaf 1-DNH in the aspects of medicine and healthcare products.

The invention relates to a method for extracting leaf protein from alfafa hay, aiming at providing a preparation method which can realize mass production and has the advantages of no time limit, simple process, low cost and high efficiency. In the method provided by the invention, leaf protein is extracted by acidification heating method and salt treatment. The method comprises the following steps of: smashing air-dried or kiln-dried alfalfa, adding appropriate amount of sodium pyrosulphate, adding water in the ratio of material to water being 1: (10-40), preimpregnating for 12-36 hours and then beating for 5 minutes, filtering (or centrifuging) with a 120-mesh filter cloth to obtain green juice, adjusting pH value to 2-7 by utilizing 2% sodium hydroxide and 0.5 mol / L hydrochloric acid solution, rapidly heating to the temperature of 40-80 DEG C in a boiling water bath, and then flocculating for 3-20 minutes in a constant-temperature water bath at the same temperature; and rapidly cooling to room temperature with cold water (normal temperature), standing for 1 hour, filtering (or centrifuging) to obtain leaf protein paste, and finally drying in a drying oven at the temperature of 60-70 DEG C. In the extraction method, the leaf protein is extracted from alfafa hay used as raw material; compared with the traditional method which takes fresh alfafa hay as the raw material, the cost is reduced, the yield efficiency is greatly improved, and the defect that the production time is limited by plant growth season is overcome.

The invention discloses a method for extracting black rice anthocyanins. The present invention determines the optimum black rice anthocyanin extraction conditions by changing the ethanol concentration, pH, temperature and time and orthogonal experiment by single factor, so as to improve the extraction effect of black rice anthocyanin and improve the extraction rate of black rice anthocyanin . Then the best conditions obtained by the orthogonal test were used to extract black rice anthocyanins by the three-stage countercurrent method, the effect was better and the extraction rate was higher. This test method conforms to the green and low-carbon environmental protection concept. The test materials can be recycled, which reduces the cost of test research and environmental pollution.

The invention discloses a method for extracting pepper leaf essential oil through a combination of a deep eutectic solvent method and ultrasonic-assisted distillation. According to the method, a deepeutectic solvent (DES) is formed by a hydrogen bond acceptor (HBA) and a hydrogen bond donor (HBD) according to a certain molar ratio, and essential oil is extracted from pepper leaves through ultrasonic-assisted distillation by using the DES. The method comprises the following main steps: (1) cleaning, drying and crushing fresh pepper leaves; (2) preparing the DES; (3) carrying out ultrasonic-assisted treatment on the DES and pepper leaf powder; and (4) extracting the pepper leaf essential oil. The method has the beneficial effects that optimal extraction process conditions responsive to surface optimization are as follows: a choline chloride-urea deep eutectic solvent is used as the optimal extraction agent, a liquid-to-solid ratio is 14: 1 (mL / g), microwave power is 300 W, and extraction time is 53 minutes. The extraction method is simple, and the yield of the pepper leaf essential oil is increased; essential oil components are analyzed by adopting gas chromatography-mass spectrometry; a new thought is provided for essential oil extraction method; and meanwhile, the application range of the DES is also expanded.

The invention discloses a process for extracting the protein of cordyceps militaris and an optimization method for optimal extraction conditions of the process. According to the process, the sporocarp of cordyceps militaris, which is used as a main raw material, is crushed into powder, then the powder is screened with a sieve, and the protein is extracted by an alkali extraction and acid precipitation method. The extraction conditions are optimized by single-factor experiment and response surface methodology so as to obtain the optimal extraction process. According to the method, the process is simple, cost is low, and the raw materials are wide in source. The cordyceps militaris is rich in protein, and a large amount of research on the process for extracting the protein is not provided at present; and the method provides practice guidelines for the relative large-scale production of protein and the development of relative products, and has potential market development prospect.

The invention relates to the technical field of plant polysaccharide extraction, in particular to an extraction method of xtraction method of flammulina velutipes polysaccharides. The extraction method comprises the following steps: cleaning sporocarp of flammulina velutipes, drying in the sun, grinding into powder, adding obtained flammulina velutipes dry powder into distilled water, extracting in a water bath, centrifuging an obtained extracted solution, and carrying out vacuum concentration on an obtained centrifuged supernate at 50 DEG C to 1 / 4 of the original volume. The extraction rate of the flammulina velutipes polysaccharides obtained by the method is up to 8.21%; the extraction rate of the flammulina velutipes polysaccharides is greatly improved; according to the extraction method, Saveg liquid and a sugar liquid are mixed and uniformly shaken, denatured proteins are filtered out, operation is repeated for multiple times, the content of proteins in the flammulina velutipes polysaccharides is basically zero at the moment, the high-purity flammulina velutipes polysaccharides without proteins can be obtained, and the biological activity of the flammulina velutipes polysaccharides is improved.

The invention provides novel tablet candies capable of regulating blood pressure and a preparation method thereof. The novel tablet candies capable of regulating blood pressure are prepared from 15-25parts by weight of cyclocarya paliurus, 35-55 parts by weight of golden camellia, 35-55 parts by weight of moringa leaves, 15-25 parts by weight of mongolian dandelion herb, 85-115 parts by weight ofkudzuvine roots, 10-30 parts by weight of glucose powder, 10-30 parts by weight of maltodextrin, 2-6 parts by weight of citric acid, 10-15 parts by weight of magnesium stearate, and 2-6 parts by weight of menthol. The preparation method of the novel tablet candies capable of regulating blood pressure comprises the following steps of carrying out extracting, carrying out concentrating, carrying out drying, carrying out tablet-compressing and so on; and the best extraction conditions are as follows: the water volume is 1.5 liters, extraction is performed for 4 times, and extraction each time isperformed for 1 hour. The novel tablet candies capable of regulating blood pressure solve the technical problems of novel blood-pressure-regulating tablet candies in the prior art, namely low contentof total flavonoids as an active ingredient, poor curative effects and so on. The preparation method of the novel tablet candies capable of regulating blood pressure is rational in experimental design; and the prepared finished products are high in total flavonoid content and good in stability. And thus, the prepared novel tablet candies capable of regulating blood pressure have improved quality,and are simple and convenient to use, safe, effective, and free of toxic and side effects.

The invention discloses a method for extracting pepper leaf essential oil through a combination of a deep eutectic solvent method and microwave-assisted distillation. According to the method, a deep eutectic solvent (DES) is formed by a hydrogen bond acceptor (HBA) and a hydrogen bond donor (HBD) according to a certain molar ratio, and essential oil is extracted from pepper leaves through microwave-assisted distillation by using the DES. The method comprises the following main steps: (1) cleaning, drying and crushing fresh pepper leaves; (2) preparing the DES; (3) carrying out microwave-assisted treatment on the DES and pepper leaf powder; and (4) extracting the pepper leaf essential oil. The method has the beneficial effects that optimal extraction process conditions responsive to surfaceoptimization are as follows: a choline chloride-urea deep eutectic solvent is used as the optimal extraction agent, a liquid-to-solid ratio is 16: 1 (mL / g), microwave power is 480 W, and extraction time is 53 minutes. The extraction method is simple, oil yield is high, and effective components in the essential oil are rich; the essential oil components are analyzed by adopting gas chromatography-mass spectrometry; a new thought is provided for essential oil extraction method; and meanwhile, the application range of the DES is also expanded.

The invention discloses an extraction method of selenium polysaccharide from pear pulp, which includes: drying and pulverizing a sample, and performing ultrasonic assistance-combined solvent continuous extraction method for extracting the selenium polysaccharide, wherein the sample is successively extracted with distilled water, a HCl solution or an alkali solution to obtain the selenium polysaccharides from the pear pulp, in different dissolubilities. In the invention, for the first time, the ultrasonic assistance extraction is combined, thus issuing the novel selenium polysaccharide extraction method - ultrasonic assistance-combined solvent continuous extraction method. In the method, the distilled water, HCl solution and NaOH solution are successively used for extracting the water soluble, acid soluble and alkali soluble selenium polysaccharides under the ultrasonic assistance. The method is high effective in selenium polysaccharide extraction and has extensive market promotion value.

The invention provides an ultrasonic-assisted method for extracting total flavonoid in alfalfa.The method comprises the following steps: (1) alfalfa is dried and smashed to obtain alfalfa powder; (2) an organic solvent (such as petroleum ether) is added to the alfalfa powder obtained in the step (1), heating reflux is performed for 4-24 hours, filtering is performed so as to remove the organic solvent, the alfalfa powder is obtained, and then the alfalfa powder is dried; (3) an ethanol solution with the volume fraction ranging from 20% to 70% and the alfalfa powder obtained in the step (1) are mixed according to the liquid-to-solid ratio of mL:g ranging from (20:1) to (70:1), extraction is performed at the temperature of 30 DEG C to 80 DEG C and under ultrasonic assistance, and an alfalfa total flavonoid extraction solution is obtained.The extraction rate of the total flavonoid can be improved with the method, the ultrasonic extraction is a physical process, the optimum temperature of the extraction process is about 60 DEG C, active ingredients of the flavonoid material in the alfalfa can be prevented from being changed by high temperature, and the extraction efficiency of the total flavonoid in the alfalfa is improved.

The invention discloses a Bo-chrysanthemum total flavone extract preparation method based on response surface optimization. The preparation method is characterized by comprises the following steps: grinding Bo-chrysanthemum, adding ethanol, carrying out reflux extraction, cooling the extract, subjecting the extract to centrifugation, and collecting the supernate namely the Bo-chrysanthemum total flavone extract; wherein the optimal conditions that influence the reflux extraction effect are determined through response surface experiments. The provided extraction method has the advantages that the operation is convenient and fast, the extraction cost is low, and no toxic or harmful substance is introduced into the final product (Bo-chrysanthemum wine). The optimal extraction factors are determined by the response surface design and optimization, and thus the optimal extraction factors are scientific.

The invention discloses a sweet potato health-care rice with an anti-oxidizing function and a preparation method thereof. The sweet potato health-care rice is prepared from the following components in percentage by mass: 35 to 45% of high-resistance starch rice, 45 to 55% of sweet potato flour, 1 to 2% of compound nutrition supplement, 2 to 4% of blue strawberry fruit powder, and 5 to 15% of sweet potato anthocyanin concentrate, wherein the compound nutrition supplement is prepared by compounding selenium, zinc, calcium, vitamin E, inositol niacinate and dietary fiber according to a mass ratio of 1:1:2:3:1:4. The sweet potato health-care rice has the advantages that by optimizing multiple conditions, the nutrition components of the sweet potato flour are maintained, the mouth feel of rice noodles is guaranteed, the eating is smooth, the biting is easy, and the eating is convenient; the nutritional value is high, the mouth feel is better, and the sweet potato health-care rice is easily popularized by people.

The invention discloses a peony flavone extract as well as a preparation method and application thereof. The preparation method of the peony flavone extract provided by the invention comprises the following steps: performing low-temperature drying and crushing on low-temperature frozen peony flowers, adding ultrapure water according to a material-to-liquid ratio of 1: 20g / mL-1: 60g / mL, performing ultrasonic extraction for 20-60 minutes under the conditions that the power is 100W-500W and the temperature is 40-70 DEG C, collecting extract liquid, performing grinding by a colloid mill, performing homogenizing and centrifuging to obtain supernate, and performing freeze drying on the supernate to obtain the peony flavone extract. And performing membrane separation on the supernate to obtain filtrate, and concentrating to obtain the peony flavone extract. Compared with the traditional extraction method, the ultrasonic-assisted extraction method has the advantages of time saving, high extraction rate, simple equipment, low energy consumption and the like. The peony flower extract effervescent tablet and the peony flower peptide drink are prepared according to the physicochemical properties of the active ingredients of the peony flowers, so that the value of the peony flowers can be fully utilized, and the peony flower peptide drink has a wide development prospect.

The invention discloses an extracting method of selenoprotein in a pear fruit. The method comprises the steps that pear pulp is taken to be dried and grounded into powder, a mild continuous extractingmethod is adopted, and the continuous extracting method is characterized in that distilled water, a NaCl solution, ethanol and monometallic sodium orthophosphate-monopotassium phosphate buffering liquid are taken as solvents to sequentially perform extraction to obtain pear fruit selenoprotein with different dissolvability. According to the extracting method, milder extracting and stirring extracting manners are adopted to extract pear pulp selenoprotein, the yield is high, extraction is conducted through ultrasound and mechanical vibration, the recovery rate is lower, water-solubility selenoprotein, salt-solubility selenoprotein and alcohol-solubility selenoprotein are extracted under the mild extraction taking the distilled water, the NaCl solution, the ethanol and the monometallic sodium orthophosphate-monopotassium phosphate buffering liquid as the solvents, and the method capable of efficiently extracting selenium polysaccharide has wider marketing popularization value.

The invention discloses a method for extracting and purifying polysaccharide of large leaf moss from the large leaf moss. The method comprises the following steps of: crushing the large leaf moss, extracting crude polysaccharide from medicinal materials with water in an ultrasonic extractor, carrying out decentralization, filtering, concentration, protein removing to the extract, using alcohol toprecipitate out of water extract, obtaining water-soluble crude polysaccharide of the large leaf moss, further carrying out separation and purification to the crude polysaccharide, vacuum-drying and obtaining the refined product of polysaccharide of the large leaf moss. The method can extract the polysaccharide of the large leaf moss from the large leaf moss to a maximum extent, is safe, non-toxicand reliable, is easy to operate, has high yield and provides possibility for exerting the drug effect of the polysaccharide of the large leaf moss and preparing drugs or health foods for various indications.

The invention provides a method for extracting flavonoid compounds from lamiophlomis rotata by a response surface method, which comprises the following steps: A) pulverizing lamiophlomis rotata, and screening to obtain medicinal material powder; B) adding the medicinal material powder into a 10-90% ethanol solution according to a solid-to-liquid ratio of 1: 10-1: 50g / mL, soaking for 2-8h, carryingout ultrasonic extraction for 10-30min, and filtering to obtain a flavonoid compound extracting solution; wherein the ultrasonic extraction conditions of the soaking time, the ethanol volume fractionand the solid-to-liquid ratio are optimized by adopting a response surface method. The extraction process for extracting flavone from lamiophlomis rotata by an ultrasonic method is optimized throughsingle factor and response surface method experiments, so that the optimal extraction condition is obtained. The method solves the problem of low extraction rate of the total flavonoids in the lamiophlomis rotata by a traditional method, and provides a theoretical basis for large-scale production of the flavonoids in the lamiophlomis rotata and development of flavonoid compounds in plants.

The invention relates to research of an extract of total flavone of eucommia bark, and in particular to an extraction method of total flavone of eucommia bark and an application of the total flavone of eucommia bark in antioxidants and hypoglycemic agents. According to the invention, eucommia bark is taken as a raw material to extract total flavone in the eucommia bark by a microwave-assisted method, wherein an optimal extraction condition of the total flavone is determined by setting a single-factor experiment and optimizing a response surface. The invention provides the application of the total flavone in oxidation resistance and hypoglycemic activity.

The invention relates to a method for extracting and separating flavonoid substances from soil. The method comprises the steps of (1) drying soil samples, and performing screening; (2) adding an extracting solution, performing uniform mixing, performing ultrasonic extraction, performing centrifuging, collecting supernatant, repeatedly operating the steps on centrifuged precipitate, combining supernatant, and performing evaporation to remove methanol, wherein the extracting solution consists of EDTA-2Na and methanol, and the mass ratio of the EDTA-2Na to the soil samples is (0.028-0.32) to 1; and (3) performing purification so as to obtain the flavonoid substances. The inventor finds that when the EDTA-2Na and the methanol are added to soil in a specific proportion, various flavonoid substances can be sufficiently extracted in a short time, and remarkable technical effects can be obtained.

The invention belongs to the field of extraction of plant nutritional ingredients and particularly relates to a response surface analysis based method for extracting soluble protein from leaves of non-heading Chinese cabbage. According to the method, a single-factor experiment is performed, a standard solution is prepared, a standard curve is drawn, a light absorption value is measured at the wavelength of 595 nm, and a regression equation is calculated; 1 g of fresh leaves of non-heading Chinese cabbage are weighed and put in a mortar, 2 ml of a phosphoric acid buffer solution is added, the mixture is ground into homogenate, the homogenate is transferred into a centrifugal tube, the mortar is washed separately with 6 ml of the phosphoric acid buffer solution, a washing solution is collected in the same centrifugal tube and left to stand at the temperature of 25 DEG C for 45 min, precipitates are removed through centrifugal treatment, a liquid supernatant is transferred into a 10 ml volumetric flask, the phosphoric acid buffer solution is added to the metered volume at the scale, and a to-be-measured sample extracting solution is obtained; 0.1 ml of a protein extracting solution issucked, 5 ml of a coomassie brilliant blue reagent G250 is added, the mixture is left to stand for 2 min, then, the light absorption value at the wavelength of 595 nm is measured, and the content ofprotein in the to-be-measured sample extracting solution is calculated. The method has high experiment accuracy and good extraction effect.

A Chinese medicine in the form of capsule, an improvement on infusion, for treating skin itching is disclosed.

The invention discloses a rice vinasse polypeptide mask as well as a preparation method and application thereof, and the rice vinasse polypeptide mask comprises the following raw materials in parts by mass: 6-10 parts of 1, 3-butanediol; 3-5 parts of glycerol; 4-6 parts of a tremella fuciformis extract; 5-7 parts of a radix astragali extract; 5-7 parts of a centella asiatica extract; 10-12 parts of a rice vinasse polypeptide extract; the preparation method comprises the following steps: preparing the following components in parts by weight: 45-65 parts of deionized water, 0.2-0.5 part of phenoxyethanol, 0.3-0.5 part of xanthan gum, 0.1-0.4 part of ethylparaben and 2-4 parts of polysorbate-80; adding phenoxyethanol and xanthan gum into deionized water, mixing, and heating and dissolving in a water bath to obtain a solution A; mixing 1-3 butanediol, glycerol, ethylparaben and polysorbate-80, heating and dissolving to obtain a solution B; and mixing the solution A and the solution B at 80-90 DEG C, cooling to 40-50 DEG C, adding the tremella extract, the astragalus extract, the centella asiatica extract and the rice vinasse polypeptide extract, and mixing to obtain the mask. The polypeptide mask disclosed by the invention not only has the effects of moisturizing, resisting oxidation, resisting aging and whitening, but also is simple in process and low in production cost.

The invention relates to a method for preparing bitter gourd total saponins by an ultrahigh pressure assisted method and a purification method thereof. Reagents adopted by the method are free of volatility and toxicity, and saponin substances with high purity can be efficiently obtained in an environment-friendly mode. The method disclosed by the invention comprises the following steps: 1) cleaning, dicing, drying and crushing bitter gourds, and then sieving with a 60-mesh sieve to prepare bitter gourd powder; 2) weighing bitter gourd powder, and carrying out ultrahigh pressure extraction with an ethanol solution to obtain a bitter gourd total saponin extracting solution; (3) centrifuging the extracting solution, collecting supernate, carrying out rotary evaporation under the conditions of 40 DEG C and the rotating speed of 90r / min until no ethanol smell exists, and freeze-drying to obtain bitter gourd total saponin powder; 4) weighing the crude extract bitter gourd total saponin powder, and dissolving the crude extract bitter gourd total saponin powder in a 1-butyl-3-methylimidazolium tetrafluoroborate solution according to a material-liquid ratio; 5) fully stirring, adding a NaOH solution, and carrying out vortex oscillation until two phases are formed; 6) centrifuging under the conditions of 4 DEG C and 4000 r / min until two phases are fully separated; and 7) collecting a NaOH lower phase rich in saponin, neutralizing with hydrochloric acid, and freeze-drying to obtain the total saponin.

The present invention relates to the research on the extract of Eucommia ulmoides, in particular to a method for extracting total flavonoids of Eucommia ulmoides and its application in antioxidants and hypoglycemic agents. The optimal extraction conditions of flavonoids were determined by setting up single factor experiments and response surface optimization, and the application of antioxidant activity and hypoglycemic activity of total flavonoids was provided.

The invention relates to an efficient solid-liquid reverse flow extraction device and extraction method. The device comprises a U-shaped solid-liquid reverse flow extraction device consisting of threegroups of stirring devices, wherein a solid material feeding device is arranged at one sides of the stirring devices, arranged at the two sides, of the three groups of stirring devices; a solid material cabin is arranged above the feeding device; an extraction liquid outlet is formed under the feeding device; a solid residue discharging opening is formed in the other sides of the stirring devices, arranged at the two sides, of the three groups of stirring devices; a solvent inlet is formed under the discharging opening of the solid residue; a transmission device is arranged on the three groups of stirring devices. The spiral length limitation incapable of being realized by single spiral and double spirals is lengthened by using the U-shaped structure; the defect of separation requirementof the single spiral and the double spirals is overcome; the spiral length is increased; the occupied area is not increased. The device is a novel efficient extraction device suitable for solid-liquidcontinuous reverse flow extraction. The structure is compact; the operation is simple; the work intensity is low; the automatic operation can be realized.

The invention discloses an extraction method of lily bulb flavone and an optimization method of the extraction method. The extraction method comprises the following steps of step I, crushing lily bulbs, and performing screening; step II, weighing lily bulb powder, and adding an ethanol solution of which the concentration is 50-90%, wherein the ratio of volume of the ethanol solution to the mass ofthe lily bulb powder is (4:1) to (20:1)ml / g; step III, placing the ethanol solution in which the lily bulb powder is mixed in an ultrasonic breaker of which the water bath temperature is 20-90 DEG Cand the frequency is 100Hz, performing extraction, and after extraction, performing sucking filtration, wherein number of ultrasonic times is 1-3, and the ultrasonic time is 20-100min; and step VI, after ultrasonic extraction, performing sucking filtration, merging filtrate, and concentrating the merged filtrates with a rotation evaporimeter so as to obtain the lily bulb flavone. According to theoptimization method, ultrasonic waves are used for assisting in the extraction method, and through combination of a response surface, and systems software Design Expert, the best extraction conditionfor the total flavone in the lily bulbs is optimized. The extraction rate is increased, and the technology is stable and reliable and high in maneuverability.

The invention provides a method for extracting alfalfa polysaccharide by use of complex enzyme (cellulase, papain and pectinase). The extracting temperature of the alfalfa polysaccharide is reduced, the extracting rate of the alfalfa polysaccharide is improved, and the method is suitable for popularization. The method comprises the following steps: determining the test range of factors influencing the extracting rate of the alfalfa polysaccharide through a single-factor test, determining the optimum proportion of the complex enzyme through an orthogonal test, then obtaining the relation between a response value and an influence factor by combing a central complex test with the response surface methodology (RSM), and computing the level of the influence factor when the maximum response value is obtained so as to determine the optimum extracting condition of extracting the alfalfa polysaccharide by complex enzyme, wherein the cellulase is 2.5%, the papain is 2.0%, pectinase is 3.0%, the extracting temperature is 52.7 DEG C, the extracting pH value is 3.87, the extracting solid-to-liquid ratio is 78.91, and the extracting time is 2.73 hours. The extracting rate of alfalfa polysaccharide is more than 5.0% under the condition provided by the invention.