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Extraction and purification technology of four kinds of flavone C-glycosides in moso bamboo leaves

A technology for flavonoids and flavonoid carbon glycosides from bamboo leaves is applied in the field of extraction and purification of flavonoid carbon glycosides, and can solve the problems of less optimization, low purity, and no industrialized production.

Inactive Publication Date: 2015-04-29
INT CENT FOR BAMBOO & RATTAN
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  • Application Information

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Problems solved by technology

[0009] At present, the optimization of the extraction and purification process for the four flavonoid glycosides (orientin, isoorientin, vitexin and isovitexin) of bamboo leaves is still relatively small, and the purity is relatively low, which has not yet been realized. Industrial production

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  • Extraction and purification technology of four kinds of flavone C-glycosides in moso bamboo leaves
  • Extraction and purification technology of four kinds of flavone C-glycosides in moso bamboo leaves
  • Extraction and purification technology of four kinds of flavone C-glycosides in moso bamboo leaves

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Embodiment 1

[0128] A process for extracting and purifying orientin, isoorientin, vitexin and isovitexin from leaves of Phyllostachys pubescens, comprising the following steps:

[0129] (1) Accurately weigh 2 kg of Phyllostachys pubescens leaf powder, add in ethanol aqueous solution and carry out heat reflux extraction, repeat extraction 4 times, filter and merge filtrate to obtain extract; The technology of described heat reflux extraction is: 75% ethanol, solid-liquid ratio 1:20, 70°C constant temperature water bath extraction for 2 hours;

[0130] (2) Adopt vacuum distillation to remove the organic solvent in the extract, add petroleum ether equal to the volume of the extract for extraction, remove pigment and fat-soluble components, repeat the extraction three times, and obtain 579.809g of Phyllostachys pubescens flavonoids crude extract, petroleum ether recycle, recycle;

[0131] (3) Pretreatment of macroporous resin D101: wash away fine resin and impurities with absolute ethanol, an...

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Abstract

The invention discloses an extraction and purification technology of four kinds of flavone C-glycosides in moso bamboo leaves. The active ingredients of moso bamboo leaves are extracted by a heat reflux extraction process, and the best extraction technology for obtaining four kinds of flavone C-glycosides through a single-factor experiment and an orthogonal experiment comprises the following steps: extracting with 75% ethanol for 2h at 70 DEG C according to a solid-liquid ratio of 1:20, and repeatedly extracting for 4 times; purifying the crude extract of flavone by use of macroporous resin, wherein D101 macroporous resin is the best choice according to comparison of static saturated adsorption capacity and desorption capacity; and further purifying the component by use of polyamide resin, wherein the particle size of the polyamide resin is 0.15-0.25mm according to the comparison of the dynamic adsorption screening results of three common kinds of polyamide resin, and a 40% ethanol solution is adopted for purifying the four kinds of flavone C-glycosides. Through the treatment, the final purity of the four kinds of flavone C-glycosides bamboo leaf flavone powder reaches 76.939mg.g<-1>.

Description

technical field [0001] The invention relates to a process for extracting and purifying flavone carbon glycosides, in particular to a process for extracting and purifying four kinds of flavonoid carbon glycosides in moso bamboo leaves. Background technique [0002] Due to their different structures and sources, flavonoids have very different solubility characteristics, so an appropriate solvent can be selected according to the polarity for extraction. At present, the extraction methods mainly include: water extraction, alkaline water or dilute alcohol extraction, organic solvent heat reflux extraction, microwave-assisted extraction, ultrasonic extraction, supercritical extraction, etc. [0003] The water extraction method is used for the extraction of flavonoid glycosides, which is low in cost and suitable for industrial production, but the extract contains water-soluble impurities such as proteins and polysaccharides, the extraction efficiency is low, it is prone to mildew, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04
CPCC07D407/04
Inventor 郭雪峰岳永德汤锋王进姚曦荀航孙嘏张春娟
Owner INT CENT FOR BAMBOO & RATTAN
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