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A method for extracting polysaccharides from alfalfa using compound enzymes

A technology of alfalfa polysaccharide and enzyme activity, which is applied in application, animal feed, animal feed, etc. It can solve the problems of imperfect extraction process of alfalfa polysaccharide, reduce the biological function of polysaccharide, destroy the structure of polysaccharide, etc., so as to promote the development and application and reduce the extraction time , the effect of improving the extraction efficiency

Inactive Publication Date: 2016-08-10
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Problems solved by technology

However, the extraction process of alfalfa polysaccharides is not yet perfect. The current extraction method is mainly hot water alcohol precipitation method. Through continuous heating, the plant cell walls are softened and ruptured, and the polysaccharides overflow
This method not only takes a long time to operate and the extraction rate is low, but also the continuous high temperature heating will destroy the polysaccharide structure and reduce the biological function of the polysaccharide, so the extraction process needs to be improved urgently

Method used

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  • A method for extracting polysaccharides from alfalfa using compound enzymes
  • A method for extracting polysaccharides from alfalfa using compound enzymes
  • A method for extracting polysaccharides from alfalfa using compound enzymes

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Embodiment Construction

[0031] 1. Crush the air-dried or dried alfalfa powder, pass through a 40-mesh sieve, and degrease it with petroleum ether or ether on a Soxhlet extractor until the supernatant is transparent and clear, then dry it at 40°C to obtain defatted grass powder;

[0032] 2. The cellulase, papain and pectinase used in this method were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd., and the enzyme activities were 15000U / g, 6000U / mg and 50U / g respectively.

[0033] 3, get defatted grass powder, by grass powder weight, add 2.5% cellulase, 2.0% papain, 3.0% pectinase, then add disodium hydrogen phosphate-citric acid buffer (pH =3.87);

[0034] 4. Put it in a constant temperature water bath, raise the temperature to 52.7°C, then keep the constant temperature, extract for 2.73 hours, stir once every 20 minutes during this period, filter with a vacuum pump after the action, collect the filtrate, and obtain the polysaccharide extract;

[0035] 5. Boil the polysaccharide extract f...

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Abstract

The invention provides a method for extracting alfalfa polysaccharide by use of complex enzyme (cellulase, papain and pectinase). The extracting temperature of the alfalfa polysaccharide is reduced, the extracting rate of the alfalfa polysaccharide is improved, and the method is suitable for popularization. The method comprises the following steps: determining the test range of factors influencing the extracting rate of the alfalfa polysaccharide through a single-factor test, determining the optimum proportion of the complex enzyme through an orthogonal test, then obtaining the relation between a response value and an influence factor by combing a central complex test with the response surface methodology (RSM), and computing the level of the influence factor when the maximum response value is obtained so as to determine the optimum extracting condition of extracting the alfalfa polysaccharide by complex enzyme, wherein the cellulase is 2.5%, the papain is 2.0%, pectinase is 3.0%, the extracting temperature is 52.7 DEG C, the extracting pH value is 3.87, the extracting solid-to-liquid ratio is 78.91, and the extracting time is 2.73 hours. The extracting rate of alfalfa polysaccharide is more than 5.0% under the condition provided by the invention.

Description

technical field [0001] The invention relates to a method for extracting plant active ingredient polysaccharides, in particular to a method for extracting polysaccharides from alfalfa by using cellulase, papain and pectinase. Background technique [0002] Alfalfa polysaccharides (Alfalfa Polysaccharides) are water-soluble polysaccharides extracted from alfalfa stems and leaves, which have various biological activities. A large number of experiments have proved that within a certain dosage range, alfalfa polysaccharide can promote the growth of livestock and poultry, and improve the immunity, antioxidant capacity and disease resistance of livestock and poultry. In addition, alfalfa polysaccharides also have a certain curative effect on the treatment of high cholesterol, high blood sugar, arteriosclerosis and myocardial infarction, and more importantly, alfalfa polysaccharides have no toxic and side effects on the body. Based on the above advantages, health products and additi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23L29/30A23L33/125A23K10/30A23K20/163
Inventor 董晓芳佟建明王少璞
Owner INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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