Ultrahigh pressure assisted method for preparing total saponins of bitter gourd and purification method of total saponins

Abstract

The invention relates to a method for preparing bitter gourd total saponins by an ultrahigh pressure assisted method and a purification method thereof. Reagents adopted by the method are free of volatility and toxicity, and saponin substances with high purity can be efficiently obtained in an environment-friendly mode. The method disclosed by the invention comprises the following steps: 1) cleaning, dicing, drying and crushing bitter gourds, and then sieving with a 60-mesh sieve to prepare bitter gourd powder; 2) weighing bitter gourd powder, and carrying out ultrahigh pressure extraction with an ethanol solution to obtain a bitter gourd total saponin extracting solution; (3) centrifuging the extracting solution, collecting supernate, carrying out rotary evaporation under the conditions of 40 DEG C and the rotating speed of 90r / min until no ethanol smell exists, and freeze-drying to obtain bitter gourd total saponin powder; 4) weighing the crude extract bitter gourd total saponin powder, and dissolving the crude extract bitter gourd total saponin powder in a 1-butyl-3-methylimidazolium tetrafluoroborate solution according to a material-liquid ratio; 5) fully stirring, adding a NaOH solution, and carrying out vortex oscillation until two phases are formed; 6) centrifuging under the conditions of 4 DEG C and 4000 r / min until two phases are fully separated; and 7) collecting a NaOH lower phase rich in saponin, neutralizing with hydrochloric acid, and freeze-drying to obtain the total saponin.

Description

technical field [0001] The invention belongs to the technical field of food and medical treatment, and relates to an ultra-high pressure assisted method for preparing total bitter melon saponins and a purification method thereof. Background technique [0002] Saponins are a class of compounds composed of isoprene-derived aglycones covalently linked to one or more sugar groups. Because the lipophilic saponin in saponin is connected to the hydrophilic sugar side chain, saponin has lipophilicity and hydrophilicity, and its aqueous solution will produce a continuous foam similar to soap when shaken, so it is named saponin. Most known saponins are plant-derived secondary metabolites, but several saponins have also been found in marine animals such as sea cucumbers and starfish. [0003] Momordica saponin is a kind of glycoside compound whose aglycone is triterpene or steroidal compound. It is composed of sugar and aglycone. The difference of each kind of Momordica saponin is tha...

AI Technical Summary

Problems solved by technology

However, bitter melon saponins are mostly cucurbitane-type triterpenoids, which have high hydrophilicity, complex components and difficult separation of impurities. The above-mentioned methods for purifying saponins mostly use organic solvents, whose volatility and toxicity are harmful to the environment and human health. Unfavorable, it is difficult to obtain high-purity saponins efficiently and environmentally friendly

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