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Peony flavone extract as well as preparation method and application thereof

A technology for flavonoids and extracts of peony is applied in the field of flavonoids of peony and preparation thereof, and can solve the problems of preparing effervescent tablets that are convenient to carry without the extracts of peony flowers, and achieves convenient taking, simple equipment and low energy consumption. Effect

Pending Publication Date: 2022-04-22
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a health-care plant resource that is both edible and medicinal, peony is gradually entering the market. Most of its edible methods are making tea, boiling water, brewing wine, stewing meat, etc.; in terms of dosage forms, the current research It still stays in the traditional dosage forms such as oral liquid and decoction, but there is almost no report on preparing the peony flower extract into an effervescent tablet dosage form that is easy to carry and has a rapid effect

Method used

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  • Peony flavone extract as well as preparation method and application thereof
  • Peony flavone extract as well as preparation method and application thereof
  • Peony flavone extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1, preparation flavonoid extract in peony flower

[0037] 1. Preparation of Standard Curve

[0038] (1) Preparation of reference substance solution

[0039] Accurately weigh 10.0 mg of rutin, dissolve it with 70% ethanol, dilute to 50 mL, and prepare a 0.2 mg / mL reference solution.

[0040] (2) Preparation of color developer

[0041] Accurately weigh AlCl 3 ·6H 2 O 2.42g, dilute to 100mL with 70% ethanol, and make 0.1mol / mL developer solution.

[0042] (3) Draw a standard curve

[0043] Precisely pipette 0, 0.5, 1.0, 1.5, 2.0, 2.5mL solution from the rutin reference solution, add 1mL, 0.1mol / L color reagent, and dilute to 25mL with 70% ethanol. Shake well, fully develop the color for 15 minutes, and measure the absorbance at 510nm. A standard curve was prepared using the reference solution without rutin as a control.

[0044] 2. Determination of flavonoid content in samples

[0045] Dry the peony flowers frozen at low temperature (-10°C, -32.5°C or -...

Embodiment 2

[0087] Embodiment 2, prepare peony flower peptide drink

[0088] 0.2 kg of peony flower flavonoid extract prepared in Example 1 was fully dissolved in 7 kg of ultrapure water, and 437.5 g of collagen peptide powder, 10.5 g of vitamin C, 1.4 g of steviol glycosides, and 3.5 g of potassium sorbate were added successively, wherein , peony flower flavonoid extract, collagen peptide powder, vitamin C, steviol glycoside, potassium sorbate, and water in a mass ratio of 200:437.5:10.5:1.4:3.5:7000, fully mixed, homogenized, filled , Sterilization, that is, to obtain a natural, healthy, nutritious and palatable peony flower peptide drink rich in flavonoids and collagen peptides.

[0089] After testing and analysis, in the three batches of peony flower peptide drink products, the content of total flavonoids and total protein, the characteristic index components, were higher than 0.10 mg / mL and 30 mg / mL, respectively, and the solid content was higher than 0.10 mg / mL. The indicators are ...

Embodiment 3

[0090] Embodiment 3, prepare peony effervescent tablet

[0091] Citric acid, Wuhan Yinfei Chemical Co., Ltd.; sodium bicarbonate, Hunan Yuhua Chemical Co., Ltd.; lactose, Zhengzhou Best Food Additive Co., Ltd.; aspartame, Anhui Yuanzheng Bioengineering Co., Ltd.; polyethylene glycol 6000, Zhengzhou Renheng Chemical Products Co., Ltd.; Glucose, Shandong Ruisheng Chemical Co., Ltd.; Lycium barbarum fruit powder and seabuckthorn fruit powder purchased from Qinghai Kangpu Co., Ltd.; Rutin, China Pharmaceutical and Biological Products Control Institute; Methanol, Shandong Yuwang Reagent Company ; Ethanol, Tianjin Damao Chemical Reagent Factory; Trichloroacetic acid (TCA), Tianjin Damao Chemical Reagent Factory; all reagents were of analytical grade. Food additives potassium sorbate, steviol glycoside, and vitamin C were purchased from Henan Wanbang Industrial Co., Ltd.

[0092] (1) Preparation process optimization

[0093] 1. Preparation of Peony Flavonoid Extract

[0094] The p...

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Abstract

The invention discloses a peony flavone extract as well as a preparation method and application thereof. The preparation method of the peony flavone extract provided by the invention comprises the following steps: performing low-temperature drying and crushing on low-temperature frozen peony flowers, adding ultrapure water according to a material-to-liquid ratio of 1: 20g / mL-1: 60g / mL, performing ultrasonic extraction for 20-60 minutes under the conditions that the power is 100W-500W and the temperature is 40-70 DEG C, collecting extract liquid, performing grinding by a colloid mill, performing homogenizing and centrifuging to obtain supernate, and performing freeze drying on the supernate to obtain the peony flavone extract. And performing membrane separation on the supernate to obtain filtrate, and concentrating to obtain the peony flavone extract. Compared with the traditional extraction method, the ultrasonic-assisted extraction method has the advantages of time saving, high extraction rate, simple equipment, low energy consumption and the like. The peony flower extract effervescent tablet and the peony flower peptide drink are prepared according to the physicochemical properties of the active ingredients of the peony flowers, so that the value of the peony flowers can be fully utilized, and the peony flower peptide drink has a wide development prospect.

Description

technical field [0001] The invention relates to the preparation of the extract, in particular to the peony flavone extract and its preparation method and application. Background technique [0002] Peony is a perennial deciduous shrub of Paeoniaceae Paeoniaceae, but its wild species are only discontinuously distributed in some areas of my country. It is native to the mountains or hills in the Yangtze River Basin and the Yellow River Basin in my country. It has high ornamental value and medicinal Use value. Peony grows slowly and has a small plant type. It is a unique woody and precious flower in my country. It has thousands of years of natural growth and more than 1,500 years of artificial cultivation history. . The dried root bark of peony is used as a traditional Chinese medicine paeonol, which was first recorded in the Eastern Han Dynasty "Shen Nong's Herbal Classic". It is listed as a middle-grade product. It tastes bitter and slightly cold. , Warm poison spot, vomiting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L33/18A23L2/38A23L2/66A23P10/28
CPCA23L33/105A23L33/18A23L2/38A23L2/66A23P10/28A23V2002/00A23V2250/2116A23V2300/48A23V2250/55A23V2200/324A23V2200/30A23V2200/328
Inventor 刘政安舒庆艳刘增根彭丽平李旸
Owner INST OF BOTANY CHINESE ACAD OF SCI
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