Response surface analysis based method for extracting soluble protein from leaves of non-heading Chinese cabbage

Abstract

The invention belongs to the field of extraction of plant nutritional ingredients and particularly relates to a response surface analysis based method for extracting soluble protein from leaves of non-heading Chinese cabbage. According to the method, a single-factor experiment is performed, a standard solution is prepared, a standard curve is drawn, a light absorption value is measured at the wavelength of 595 nm, and a regression equation is calculated; 1 g of fresh leaves of non-heading Chinese cabbage are weighed and put in a mortar, 2 ml of a phosphoric acid buffer solution is added, the mixture is ground into homogenate, the homogenate is transferred into a centrifugal tube, the mortar is washed separately with 6 ml of the phosphoric acid buffer solution, a washing solution is collected in the same centrifugal tube and left to stand at the temperature of 25 DEG C for 45 min, precipitates are removed through centrifugal treatment, a liquid supernatant is transferred into a 10 ml volumetric flask, the phosphoric acid buffer solution is added to the metered volume at the scale, and a to-be-measured sample extracting solution is obtained; 0.1 ml of a protein extracting solution issucked, 5 ml of a coomassie brilliant blue reagent G250 is added, the mixture is left to stand for 2 min, then, the light absorption value at the wavelength of 595 nm is measured, and the content ofprotein in the to-be-measured sample extracting solution is calculated. The method has high experiment accuracy and good extraction effect.

Description

technical field [0001] The invention belongs to the field of extracting plant nutrient components, and in particular relates to a method for extracting soluble protein from non-heading cabbage leaves based on response surface analysis. Background technique [0002] Non-heading cabbage is a staple vegetable in my country and plays an important role in daily life. Soluble proteins refer to proteins that can be dissolved in water or other solvents in the form of small molecules. It is usually used as an important indicator in experiments such as plant physiology, microorganisms, and food processing. After heavy metal ions enter the plant, they combine with other compounds to form metal complexes or chelates, which inhibit various metabolic activities of plants, especially protein synthesis. Therefore, soluble protein content is also an important indicator to measure whether plants are under heavy metal stress. [0003] The determination of protein content by Coomassie brilli...

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of insufficient extraction in the prior art, the present invention provides a method for extracting soluble protein from non-heading cabbage leaves based on response surface analysis. Response surface analysis is an effective method for optimizing process conditions and can be used to determine The influence of each factor and its interaction on the index response value in the process, accurately express the relationship between the factor and the response value, and then improve the accuracy of the experiment and the extraction effect

Images