51results about How to "Avoid centrifugation" patented technology

The present invention relates to a process for recovering individual polymers, such as nylon and polyolefin, from a nylon-containing source such as a carpet. The process includes the steps of shredding a nylon fiber source such as a carpet wherein the shredding is conducted in the presence of a liquid that wets the carpet, granulating the wet shredded carpet slurry, further diluting the granulated slurry by adding additional water, subsequently refining the fibers and removing substantial amounts of polypropylene fibers from the slurry, preferably using a lamella clarifier, and recovering the nylon fibers by hydrocloning the slurry.

The invention relates to an automatic filling machine for beverage bottles. The automatic filling machine for the beverage bottles comprises a rack, a conveying chain, a conveying motor, a guardrail,a flushing device, a liquid injection device and a gland device, wherein the liquid injection device comprises a liquid injection rack, a bottle inlet and outlet guide device is arranged on the liquidinjection rack, the bottle inlet and outlet guide device comprises a bottle guide groove, a bottle outlet turntable and a bottle inlet turntable, the liquid injection device further comprises a liquid injection turntable, a liquid injection bottle clamping rotating ring is arranged on the liquid injection turntable, a liquid injection gun is connected to the liquid injection turntable through a liquid injection telescopic supporting rod, the liquid injection gun comprises a vacuum suction pipe connected with the telescopic supporting rod, the upper part of the vacuum suction pipe is providedwith a suction pipe joint, a liquid injection pipe is arranged in the vacuum suction pipe, and the upper part of the liquid injection pipe penetrates through the vacuum suction pipe and is connected to a liquid injection box. According to the automatic filling machine for the beverage bottles, the liquid injection gun is designed to be a structure in which the liquid injection pipe is sleeved withthe vacuum suction pipe, when the liquid injection gun is inserted into bottle bodies, the liquid injection of the beverage bottles in the vacuum state can be realized, meanwhile, the beverage bottles are adsorbed on the liquid injection gun, the centrifugation in the rotating process is avoided, the structural cost of the turntables is saved, and the matching difficulty is reduced.

The invention discloses a method for extracting and purifying DNA, comprising the following steps: 1) pre-treatment: a biological sample is in contact with pre-treated lysis solution, and solid substance adhering to biological cells is removed to obtain rude lysis solution; 2) nucleic acid adsorption: the rude lysis solution obtained in step 1 is mixed with lyse combined liquid and magnetic bead suspension to form a solution system containing magnetic nanosphere-DNA composite body, and the magnetic nanosphere-DNA composite body in the solution system is collected; 3) washing: the magnetic nanosphere-DNA composite body obtained in step 2 is successively washed with a cleaning solution I and a cleaning solution II, and then DNA is dissolved out by spent regenerant. The method provided by the invention has 90% of the DNA extracting efficiency, the extracted DNA can be used in down stream analysis operation, such as STR multiplex amplification, DNA sequencing, DNA quantitation and the like.

The invention discloses a method for extracting and purifying spittle DNA, which comprises the following: 1) a step of pre-treatment, which is to contact the spittle with pretreatment lysis solution, remove solid matters adhered onto cells and obtain coarse lysis solution; 2) a step of nucleic acid absorption, which is to mix the coarse lysis solution obtained by the step 1) with lysis binding solution and magnetic bead suspension to form a solution system containing magnetic nano microsphere and DNA composite and is to collect the magnetic nano microsphere and DNA composite from the solution system; and 3) a step of washing, which is to wash the magnetic nano microsphere and DNA composite obtained by the step 2) with washing liquid I and washing liquid II in turn, and dissolve out DNA by using eluent. When the method provided by the invention is used, the DNA extraction efficiency may reach 90 percent, the extracted DNA can be used in short tandem repeat (STR) composite amplification, DNA sequencing, DNA quantification and other downstream analysis and operation.

The invention discloses a reagent for extracting and purifying DNA, comprising the following substances: preprocessing lysis solution with pH of 7.4-8.5: water serves as solvent, and solute comprises the substances with the following final concentration: 10-100mM of buffer salt, 0.5%-5% of surface active agent, 1-100mM of chelating agent, 50-150mM of soluble salt, and 0.2-4mg / ml of protease K; pyrolysis combined liquid with pH of 6.4-7.4: water serves as solvent, and solute comprises the substances with the following final concentration: 20-150mM of buffer salt, 3-8 of MChaotropic salt, 1-10%of surface active agent, 0.5-4% of amphoteric ion detergent, 10-100mM of chelating agent and 15-30% of alcohol; cleaning solution I: water serves as solvent, and solute comprises the substances with the following final concentration: 4-6 of MChaotropic salt, 25-50% of alcohol and 10-100mM of chelating agent; cleaning solution II is 75% of ethanol water solution; spent regenerant is TE (10mM Tris-HCl, 1mMEDTA, and pH is 8.0); magnetic bead suspension is nanoscale silicone coated magnetic microsphere (50-100mg / ml).The DNA extracting efficiency is as high as 93.67% by adopting the reagent.