A determination method of the 1-deoxynojirimycin content in silkworm
powder relates to silkworm
powder. The method comprises the following steps: weighing two 1-deoxynojirimycin control samples used as control liquid 1 and 2; sucking the control liquid 1 and 2, injecting the samples; weighing silkworm
powder to be determined, adding
petroleum ether, performing heating
reflux and extraction in water bath, filtering, removing the filtrate, volatilizing the
petroleum ether to
dryness to obtain a product A; adding
methanol in A, performing ultrasonic extraction, filtering, concentrating the filtrate, adding in a neutral
alumina column, performing
elution, collecting the eluted solution, evaporate to
dryness, dissolving by
methanol and obtaining a constant volume so as to used the solution as a
test solution; performing
HPLC analysis of the
test solution, calculating the 1-deoxynojirimycin content in the silkworm powder to be determined by an external standard two-
point method according to a
peak area value. The method has the advantages of high precision, good
repeatability and stability, qualified sample
recovery rate, simplicity and practicality, and the like, can be popularized in general laboratories, and is applicable to the
quality control of 1-deoxynojirimycin in silkworm powder products.