High efficiency liquid chromatography detection method for content of raddeanin A in rhizoma anemones raddeanae
A technique of high performance liquid chromatography and cylindronate, which is applied in the field of detection of the two ends of traditional Chinese medicine, can solve the problems of complicated operation, low sensitivity, poor reproducibility, etc., and achieves the effects of simple operation method, simple method and saving detection cost.
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Embodiment 1
[0019] (1) Chromatographic conditions and system suitability test: as filler; mobile phase: acetonitrile-water 80:20; detection by evaporative light scattering detector; flow rate: 0.2ml / min; drift tube temperature detected by evaporative light scattering detector : 60°C, air flow rate: 1.0L / min.
[0020] (2) Preparation of reference substance solution: accurately weigh out an appropriate amount of bamboo joint A reference substance, add methanol to make a solution containing 0.4mg per 1ml, and get it;
[0021] (3) Preparation of the test solution: Take 2g of the sharp powder at both ends, place it in a conical flask with stopper, add 50ml of methanol, close it tightly, weigh it, ultrasonically treat it for 0.5 hours, let it cool, and weigh it again. Add methanol to make up the lost weight, shake well, and filter; measure 10ml of the continued filtrate, evaporate to dryness in a water bath, add methanol to the residue to dissolve, transfer to a 10ml volumetric flask and dilute to t...
Embodiment 2
[0024] (1) Chromatographic conditions and system suitability test: octadecylsilane-bonded silica gel as filler; mobile phase: acetonitrile-water 86:14; detection by evaporative light scattering detector; flow rate: 0.6ml / min; evaporation Drift tube temperature detected by light scattering detector: 90°C, air flow rate: 2.0 L / min.
[0025] (2) Preparation of reference substance solution: accurately weigh out an appropriate amount of bamboo joint A reference substance, add methanol to make a solution containing 0.4mg per 1ml, and get it;
[0026] (3) Preparation of the test solution: Take 5g of the sharp powder at both ends, place it in an Erlenmeyer flask with stopper, add 50ml of methanol, close it tightly, weigh it, ultrasonically treat it for 0.8 hours, let it cool, and weigh it again. Add methanol to make up the lost weight, shake well, and filter; measure 10ml of the continued filtrate, evaporate to dryness in a water bath, add methanol to the residue to dissolve, transfer to a...
Embodiment 3
[0029] (1) Chromatographic conditions and system suitability test: octadecylsilane bonded silica gel as filler; mobile phase: acetonitrile-water 93:7; detection by evaporative light scattering detector; flow rate: 1ml / min; evaporating light The drift tube temperature detected by the scattering detector: 130°C, and the air flow rate: 3.1 L / min.
[0030] (2) Preparation of reference substance solution: accurately weigh out an appropriate amount of bamboo joint A reference substance, add methanol to make a solution containing 0.4mg per 1ml, and get it;
[0031] (3) Preparation of the test solution: Take 8g of the sharp powder at both ends, place it in a stoppered Erlenmeyer flask, add 50ml of methanol, close it tightly, weigh it, ultrasonically treat it for 1 hour, let it cool, and weigh it again. Add methanol to make up the lost weight, shake well, and filter; measure 10ml of the continued filtrate, evaporate to dryness in a water bath, add methanol to the residue to dissolve, transf...
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