A preparing method of an edible apple tree pesticide is disclosed. The method includes weighing stemona root, tobacco eaves, oil tea leaves, Chinese gall, common cnidium fruit, celastrus angulatus, garlic, fresh ginger, hot pepper, sugar-apple, polygonum hydropiper L., and physostigma venenosum Balf. according to prescription amounts, primarily crushing the weighed raw materials, then performing ultramicro-pulverization, adding water and active polypeptide into the pulverization product, performing microwave extraction, performing centrifugation, collection a liquid supernatant, distilling and concentrating the obtained liquid supernatant, then adding alpha-terpilenol into a concentration product, fully mixing the mixture and performing filling operation to obtain the pesticide. Beneficial effects of the pesticide are that the components of the pesticide are derived from plants so that the pesticide is free of medicine residues after the pesticide is sprayed, picked apples can be directly eaten, the pesticide is nontoxic to human bodies, and a safe effective novel variety for production of green foods is provided; the pesticide can rapidly kill common pests of apple trees and drug resistance is not generated; the preparing method is simple, safe, energy-saving, and pollution-free; production equipment is simple; and investment is saved.