64 results about "Rotavirus Infections" patented technology
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A rotavirus infection usually starts within two days of exposure to the virus. Initial symptoms are a fever and vomiting, followed by three to seven days of watery diarrhea.
The present invention relates to a Lactobacillus reuteri variant from animal sources that inhibits rotavirus infection and other pathogenic microorganisms, as well as being tolerant of gastric and bile acids. Also, this invention relates to a prophylactic and therapeutic composition comprising the same for contributing in many probiotic ways to the host's general health and preventing and treating diseases or conditions associated with rotavirus and other enteric pathogens.
The invention belongs to the technical field of microorganisms, and discloses a Real-time PCR quantitative detection method for human group H rotaviruses. An upstream primer F for Real-time PCR quantitative detection comprises SEQ ID No.1 and a sequence which at least has 70% of homology with the SEQ ID No.1; a downstream primer R comprises SEQ ID No.2 and a sequence which has at least 70% of homology with the SEQ ID No.2; and the probe comprises SEQ ID No.3 and a sequence which has at least 70% of homology with the SEQ ID No.3. The Real-time PCR circulation conditions are as follows: reverse transcription is performed for 5-8 minutes at 48-55 DEG C, PCR initial activation is performed for 20-50 seconds at 95 DEG C, denaturation is performed for 10-20 seconds at 95 DEG C, annealing and extension are performed for 50-70 seconds at 55-65 DEG C, 35-45 cycles are performed, and a fluorescencesignal is detected after each cycle. The method can be used for rapidly and effectively detecting the human group H rotavirus infection. The detection method provided by the invention has relatively high detection sensitivity and specificity, is simple to operate and convenient to apply, and provides feasible technical support for group H rotavirus monitoring and group H rotavirusinfection disease prevention and control in the fields of clinical diagnosis, inspection and quarantine and the like.