Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of bifidobacterium animalis subsp. Lactis BLa80 in preparation of medicine or food for resisting diarrhea caused by rotavirus infection

A technology of animal bifidobacteria and rotavirus, applied in the field of microorganisms, to achieve the effect of preventing and alleviating diarrhea symptoms and excellent anti-rotavirus infection

Active Publication Date: 2022-03-08
JIANGSU WECARE BIOTECHNOLOGY CO LTD
View PDF14 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current research on the anti-RV activity of probiotics is still in the early stage, and further exploration is needed to broaden the application prospects of probiotics in viral diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of bifidobacterium animalis subsp. Lactis BLa80 in preparation of medicine or food for resisting diarrhea caused by rotavirus infection
  • Application of bifidobacterium animalis subsp. Lactis BLa80 in preparation of medicine or food for resisting diarrhea caused by rotavirus infection
  • Application of bifidobacterium animalis subsp. Lactis BLa80 in preparation of medicine or food for resisting diarrhea caused by rotavirus infection

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0043] This preparation example provides a kind of Bifidobacterium animalis subsp.lactis BLa80 bacterial suspension, and its preparation method is as follows:

[0044] Pick a single colony of Bifidobacterium animalissubsp.lactis BLa80 rejuvenated on the plate and inoculate it in 10 mL of TPY liquid medium, culture it anaerobically at 37°C for 12 hours, and inoculate the activated bacterial liquid in 100 mL of 2% inoculum In TPY liquid medium, culture anaerobically at 37°C for 12h, centrifuge at 5000g for 10min at 4°C, take the centrifuged bacterial pellet, resuspend with sterile PBS buffer solution, adjust the concentration of the bacterial suspension to 10 9 cfu / mL to obtain the Bifidobacterium animalis subsp. lactis BLa80 bacterial suspension, and store it at 4°C for future use.

Embodiment 1

[0046] The present embodiment carries out the mensuration of cytotoxicity to the Bifidobacterium animalissubsp.lactis BLa80 bacterial suspension obtained in Preparation Example 1, and the specific steps are as follows:

[0047] Culturing small intestinal epithelial cells: FHs 74Int cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) at 37°C, 5% CO 2 Cultured for 48 hours under the condition of passaging, digested with 0.25% trypsin for about 10 minutes. Specific steps are as follows:

[0048] Attach the cells to the wall, suck off the old culture medium in the culture vessel with a dropper or pipette, and then wash off the residual old culture medium with PBS solution; add 1.5mL digestion solution (0.25% trypsin) to the bottle and gently Shake the culture flask to make the digestive juice flow over the surface of all cells, and then observe under an inverted microscope. When the cells withdraw their protrusions and become rounded or the intercell...

Embodiment 2

[0058] This embodiment carries out virulence assay to rotavirus, with TCID 50 That is, the median tissue culture infectious dose (mediantissue culture infectious dose), refers to the amount of virus that can cause half of the cells in the culture plate or test tube to be pathological or dead, and is used to characterize the titer of rotavirus.

[0059] Propagation of rotavirus: Rhesus macaque kidney cells MA104 were passaged for 3 times, and the cells were inoculated with rotavirus hWa strain after the cells became stable. Take out the rotavirus, dilute the virus solution with 1640 culture medium (without serum), mix the diluted virus with 2 μg / mL trypsin at a ratio of 1:1 (v / v), and incubate at a constant temperature of 37°C for 45 minutes. Add 2 mL of the mixture of cultured rotavirus and trypsin to MA-104 cells, and then incubate at 37°C, 5% CO 2 Incubate in an incubator for 90 minutes. Discard the mixed solution, add 1640 culture medium (without serum) to 8 mL, and add 5...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of bifidobacterium animalis subsp. Lactis BLa80 in preparation of drugs or food for resisting rotavirus infection induced diarrhea. The medicine or food for resisting diarrhea caused by rotavirus infection comprises a bifidobacterium animalis subsp. Lactis BLa80 microbial inoculum. The bifidobacterium animalis subsp. Lactis BLa80 microbial inoculum is pure bifidobacterium animalis subsp. Lactis BLa80 or a composition of the bifidobacterium animalis subsp. Lactis BLa80 and a protective agent, and the protective agent comprises an emulsifier, polysaccharide and glycerol. The medicine or food for resisting diarrhea caused by rotavirus infection is free of toxic and side effects, free of immune stress and free of drug resistance, can inhibit infection and proliferation of rotavirus on cells in vitro, has an excellent rotavirus resisting effect, and can effectively relieve diarrhea symptoms caused by rotavirus infection.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to the application of Bifidobacterium animalis subsp. lactis BLa80 in preparing medicine or food for resisting diarrhea caused by rotavirus infection. Background technique [0002] Diarrhea (diarrhea), as one of the most common digestive system diseases, can be divided into acute diarrhea and chronic diarrhea according to the duration of the disease. The onset of acute diarrhea is sharp, and the course of the disease is within 2 to 3 weeks, most of which are caused by infection. Chronic diarrhea refers to recurrent diarrhea with a course of more than two months or an intermittent period of 2 to 4 weeks. The etiology is more complicated and can be caused by infectious or non-infectious factors. In acute diarrhea, rotavirus (RV) is a common source of infection. RV mainly attacks children under the age of 5 from November to May of the next year, and is one of the main cau...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/745A61P1/12A61P31/14A23L33/135
CPCA61K35/745A61P1/12A61P31/14A23L33/135A23V2002/00A23V2400/515A23V2200/3204Y02A50/30
Inventor 方曙光盖忠辉王桂龙朱建国
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com