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Composition for preventing or treating rotavirus infection containing licorice extract

A licorice extract, the technology of rotavirus, the application in medicine, the prevention or treatment of rotavirus infection, the prevention or improvement of rotavirus infection in the field of food compositions and viricides, can solve side effects, various Rotavirus serotypes cannot prevent problems such as inhibiting cytopathic effects and preventing or treating rotavirus infection

Inactive Publication Date: 2012-12-12
KOREA RES INST OF BIOSCI & BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this recombinant virus vaccine has been reported to cause side effects (such as intussusception) and has significant disadvantages, namely, its inability to protect against various rotavirus serotypes

Method used

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  • Composition for preventing or treating rotavirus infection containing licorice extract
  • Composition for preventing or treating rotavirus infection containing licorice extract
  • Composition for preventing or treating rotavirus infection containing licorice extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1: preparation licorice extract

[0075] 2 kg of licorice roots obtained from Jeongeup City, Jeollabukdo, Korea were added to 10 L of 100% ethanol (EtOH) and left at room temperature for 3 days. The resulting solution was then filtered through filter paper and concentrated, whereby an ethanol extract of licorice (134 g) was obtained.

Embodiment 2

[0076] Example 2: Isolation and purification of fractions and polyphenolic compounds from licorice extract

[0077] 134 g of the brown powder obtained in Example 1, which is the ethanol extract of licorice, was suspended in 1 L of water. The resulting suspension was placed in a separatory funnel and fractionally extracted with n-hexane, chloroform and ethyl acetate. Thus, an extract soluble in n-hexane (1.3 g), an extract soluble in chloroform (45 g), an extract soluble in ethyl acetate (35 g), and an extract soluble in water were obtained.

[0078] By silica gel column chromatography, using 100% chloroform, acetone and their mixed solvent (40:1 to 1:1) as mobile phase, the obtained chloroform-soluble extract 20g was further divided into 10 fractions (Fr. 1-10). Among these fractions, the third fraction (Fr.3, 3 g) was further subjected to silica gel column chromatography using a mixed solvent of n-hexane:ethyl acetate (40:1 to 2:1 (v / v)) (7g of silica gel; 230-400 mesh) to...

Embodiment 3

[0082] Embodiment 3: carry out structural analysis to compound

[0083] The molecular weight and molecular formula of the compound obtained in Example 2 were determined using a VG high-resolution GC / MS spectrometer (electron ionization mass spectrometry, Autospec Ultima). In addition, obtained by nuclear magnetic resonance (NMR) analysis (Bruker AM300,500) 1 H NMR and 13 C NMR spectrum data to determine the molecular structure of the compound.

[0084] The obtained instrumental analysis results were compared with the literature data. As a result, the compounds were found to be: isoliquiritigenin represented by Formula 1, 2-methoxyisoliquiritigenin represented by Formula 2, licocoumarone represented by Glycyrrhizin C (glyasperin C), and licoflavonol represented by formula 5 (see literature: J. Agric. Food Chem. 7408-7414, 2005; Phytochemistry, 287-293, 1998; Chem. Nat. Comp ., 389, 1972; Chem. Pharm. Bull., 1286-1292, 2000).

[0085] The specific analysis results are as fo...

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Abstract

The present invention relates to a composition for preventing or treating rotavirus infection containing licorice extract, fractions of the extract or a compound separated from the extract. The licorice extract, fractions of the extract or a compound separated from the extract have anti-rotaviral effects, and exhibit, against a variety of rotaviruses, virucidal effects and inhibitory effects on cell degeneration at the same time, and therefore can be valuably used in preventing or treating rotavirus infection.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for preventing or treating rotavirus infection, the pharmaceutical composition comprising one or more of licorice extract, its fractions or polyphenolic compounds isolated therefrom as active ingredients , the present invention also relates to a method of using the pharmaceutical composition to prevent or treat rotavirus infection. Furthermore, the present invention relates to a food composition and a virucidal agent for preventing or improving rotavirus infection, which contain one or more of licorice extract, fractions thereof, or polyphenolic compounds isolated therefrom as active Element. In addition, the present invention also relates to the application of one or more of the licorice extract, its fractions or polyphenolic compounds isolated therefrom in the preparation of medicaments for treating rotavirus infection. Background technique [0002] Globally, rotavirus has been recognize...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/236A61P31/12
CPCA23L1/1041A61K31/353A23V2250/21A61K36/484A61K31/05A23L2/54A23L1/00A23L1/3002A61K31/352A61K31/085A61K31/343A23V2002/00A23L7/198A23L33/105A61P31/12A61P31/14A61K2300/00A23V2200/324A23V2250/2132
Inventor 李雨松鲁文喆柳永培朴修珍张终仙权亨俊金夏玄郑炯在
Owner KOREA RES INST OF BIOSCI & BIOTECH
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