The invention discloses a preparation method of a
nano porous metal film and a
microelectrode sensor loaded with
glucose oxidase (GOD) on the
porous film for
blood glucose monitoring, and relates to aprocessing technology of the
porous metal film and a sensor technology.
Metal and
metal oxides of different proportions and thicknesses are subjected to magnetron co-
sputtering on a
polyimide substrate, treatment is conducted through deionized water or a weak acid solution to remove the
metal oxides, metal
layers which are each of a three-dimensional
porous network structure and are interconnected are left to serves as a support layer and a conductive layer which are loaded with the GOD, the other side of the insulated substrate is coated with Ag / AgCl to serve as a reference-counter
electrode, thus an electrochemical two-
electrode system is constituted, then the substrate is coated with a high-molecular
polymer to serve as a
diffusion-limiting layer and an anti-interference hydrophilic layer, and then a
glucose sensing microelectrode is constituted. The
nano porous metal film is large in specific surface area, the active area of the
electrode and the load amount of the GOD are effectively increased, permeability is good,
conductivity is high, and physical damage to a
microelectrode enzyme film sensing layer can be further prevented to a certain extent. The microelectrode sensor iswide in
linear range and good in long-term stability.