77 results about "Pyrrovinyquinium" patented technology

A method for extracting pyrroloquinoline quinone from fermentation broth mainly comprises the following steps: pretreating the fermentation broth to obtain a filtrate, adjusting the pH value of the filtrate to 2.5-3.5 with an acid, adding an adsorbent, and carrying out first filtration to obtain a filtrate; adjusting the pH value of the filtrate to 7.5-8, adding an adsorbent, and performing filtering for the second time to obtain a filtrate; and carrying out ultrafiltration and nanofiltration on the filtrate to obtain a nanofiltration concentrated solution, carrying out reduced pressure concentration on the nanofiltration concentrated solution, adjusting the pH value to 3-3.5, and carrying out low-temperature crystallization to obtain the product. The purity of the final product PQQ is greater than or equal to 98%, the total yield is greater than or equal to 85%, and multistage membrane equipment is adopted in the extraction process, so that the extraction operation is simpler, the automatic operation is easier, the equipment maintenance cost is reduced, the use amount of organic solvents in production is reduced, and the production cost of enterprises is reduced.

The invention discloses an application of pyrroloquinoline quinone to medicines resistant to asthma and allergic reaction. Pyrroloquinoline quinone can relieve allergic airway inflammation through antioxidant and anti-inflammatory effects, and is a potential novel medicine for the treating asthma. Pyrroloquinoline quinone has high antioxidant and anti-inflammatory effects. The in-vivo and in-vitrostudies carried out by the invention show that pyrroloquinoline quinone can reduce allergic airway inflammation by improving the immune microenvironment and regulating a JAK-STAT signaling pathway, and research shows that pyrroloquinoline quinone is safe for oral administration, so that pyrroloquinoline quinone is a potential novel medicine for treating asthma. Pyrroloquinoline quinone exists naturally in nature and can be supplemented by diet, the safety is good, the side effects are low, and the accessibility is high. Pyrroloquinoline quinone is a non-glucocorticoid immunomodulator that targets an asthma inflammatory pathway, the mechanism of medicine action is different from that of current medicines for treating asthma, and mutual complementing can be carried out.

The invention discloses a preparation method for synthesizing pyrroloquinoline quinone by a four-step method. According to the method, 4-methyl-5-nitro-2-fluoroaniline (2) is taken as a raw material,and is subjected to a ring closing reaction twice to obtain a key intermediate 5-methoxy-1H-pyrrole [2,3-f] quinoline-2,7,9-trialkyl triformate (6), and an oxidation reaction, a hydrolysis reaction and an acidification reaction are performed to obtain the pyrroloquinoline quinone (PQQ, 1). The preparation method disclosed by the invention has the advantages of environmental friendliness, easily available raw materials, low cost, simple method and suitability for industrial production, and solves the problems of high preparation cost and difficulty in industrial production in the prior art.

The invention relates to an application of pyrroloquinoline quinone (PQQ) in preparation of a medicine for preventing and treating acute altitude sickness and acute high altitude hypoxic injury. The pyrroloquinoline quinine provided by the invention has the effect of preventing and treating the acute high altitude hypoxic injury, the efficacy is equivalent to that of acetazolamide, but the acetazolamide has large toxic and side effects, and the pyrroloquinoline quinone has the advantages of low toxicity and easy acceptance by patients as a coenzyme; and in addition, through exhaustive swimmingexperiments of mice under hypoxic exposure conditions, results show that the PQQ has the characteristics of improving the physical working ability at altitude, but the acetazamide is not found to have the effect.

The invention discloses a method for producing pyrroloquinoline quinone by utilizing Gluconobacter oxydans. The steps are as follows: (1) Fermenting Gluconobacter oxydans DSM 2003 to obtain a fermentation liquid; (2) High-speed centrifugation of the fermentation liquid to obtain the supernatant Liquid, enriched by HP‑20 type macroporous resin chromatographic column to obtain the elution fraction rich in pyrroloquinoline quinone; (3) concentrating the elution fraction rich in pyrroloquinoline quinone under reduced pressure, Purified by amide column chromatography to obtain pure pyrroloquinoline quinone. The Gluconobacter oxidans strain provided by the present invention has a PQQ output of 125 mg / L under conventional culture medium and culture conditions, and the above-mentioned production and preparation method has simple conditions, quick process, and is convenient for large-scale industrial production, which is helpful for promoting the industrialization of PQQ have important meaning.

The invention discloses a method for separating and purifying pyrroloquinoline quinone in fermentation liquid by a molecularly imprinted solid-phase extraction method. According to the method, the pyrroloquinoline quinone is obtained by performing separation and purification on the fermentation liquid rich in the pyrroloquinoline quinone through a pyrroloquinoline quinone molecularly imprinted polymer. According to the method, silicon dioxide is used as a base material, the pyrroloquinoline quinone is used as a template molecule, methacrylic acid is used as a functional monomer, ethylene glycol dimethacrylate is used as a cross-linking agent, and the pyrroloquinoline quinone molecularly imprinted polymer is obtained through a thermal polymerization technique. Adsorptivity experiments prove that the pyrroloquinoline quinone molecularly imprinted polymer prepared by the method disclosed by the invention has good specific selective properties of adsorbing target objects namely the pyrroloquinoline quinone, and the largest adsorption volume can reach 10mg / mg (molecularly imprinted polymers). Solid-phase extraction is performed on the target object namely the pyrroloquinoline quinone by using the polymer as a solid-phase extraction filling, so that the purpose of efficiently separating and purifying the pyrroloquinoline quinone from the fermentation liquid is realized.