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Application of pyrroloquinoline quinone to the preparation of product with fatigue resistance and oxidative stress injury alleviating

A pyrroloquinoline quinone, anti-fatigue technology, applied in the field of pharmacy, can solve problems such as pyrroloquinoline quinone not seen

Inactive Publication Date: 2020-10-23
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the application of pyrroloquinoline quinone in delaying fatigue and alleviating oxidative stress damage has not been reported at home and abroad.

Method used

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  • Application of pyrroloquinoline quinone to the preparation of product with fatigue resistance and oxidative stress injury alleviating
  • Application of pyrroloquinoline quinone to the preparation of product with fatigue resistance and oxidative stress injury alleviating
  • Application of pyrroloquinoline quinone to the preparation of product with fatigue resistance and oxidative stress injury alleviating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1. It was found from the exhaustion time of mice that pyrroloquinoline quinone has a significant anti-fatigue function

[0038] The test method is as follows:

[0039] 1. 45 mice were adaptively fed for 1 week;

[0040] 2. Before the experimental intervention, perform adaptive swimming for 20 minutes a day for three days, and eliminate the mice that are not suitable for swimming;

[0041] 3. The remaining 40 mice were randomly divided into five groups according to body weight, 8 in each group, namely: normal control group (NC group), exhaustive exercise group (E group), pyrroloquinoline quinone (pyrroloquinoline quinone, PQQ) Intervention groups (LE group, ME and HE group).

[0042] 4. PQQ and exhaustion intervention: LE, ME and HE groups received 5, 10 and 20 mg / kg of PQQ by intragastric administration every morning, while NC and E groups received intragastric administration of the same volume of normal saline; NC group did not receive exercise intervention ...

Embodiment 2

[0047] Example 2. From serum biochemical indicators and histopathological sections, it was found that PQQ can significantly reduce the oxidative stress damage of exhausted mouse tissue

[0048] The test method is as follows:

[0049] 1. The grouping and intervention of experimental animals are the same as in Example 1. The mice were exhausted for the last time, anesthetized immediately, blood was taken from the orbit, left at room temperature for 20 minutes, centrifuged at 3000r / min, and centrifuged at 4°C for 20 minutes, and the supernatant was taken and packaged, - Store at 80°C for the determination of CK, LDH and cTnI activities.

[0050] 2. Determination of CK activity: Use a serum health disk (Wei Nano Core Technology Co., Ltd.), detect it with a fully automatic biochemical tester, and operate according to the instructions of the instrument.

[0051] 3. The LDH activity kit was purchased from Nanjing Jiancheng, and the determination was performed in strict accordance wi...

Embodiment 3

[0060] Example 3. From the oxidative stress indicators in serum and tissues, it was found that PQQ can significantly improve the anti-oxidative stress ability of exhausted mice and reduce oxidative stress damage

[0061] The test method is as follows:

[0062] 1. The grouping and intervention of experimental animals are the same as in Example 1, and the anesthesia is the same as in Example 2.

[0063] 2. The kits for the determination of biochemical indicators such as MDA, SOD, and GSH-PX in serum and tissues were purchased from Jianjian in Nanjing, and the operation was strictly in accordance with the instructions of the kits.

[0064] 3. Data statistics are the same as in Example 1.

[0065] The test results are as follows:

[0066] Effects of PQQ on oxidative stress indicators in exhausted mice

[0067] In order to further verify the antioxidative effect of PQQ on exercise-induced fatigue, the activity of GSH-Px, SOD and MDA in myocardial and liver tissues were detected....

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Abstract

The invention relates to application of pyrroloquinoline quinone to the preparation of a product with fatigue resistance and oxidative stress injury alleviating. Experiments show that pyrroloquinolinequinone has obvious effects of anti-fatigue and prolonging exercise time, and down-regulates oxidative stress to varying degrees, reduces tissue damage caused by oxidative stress, reduces inflammatory reaction caused by oxidative stress, and maintains homergy of bodies; and in a long-term contraction myotube model, pyrroloquinoline quinone maintains normal mitochondrial morphology, stabilizes mitochondrial function, and inhibits the production of reactive oxygen free radicals (ROS, reactive oxygen species), thereby reducing the damage caused by oxidative stress and delaying the occurrence offatigue, and the effect is very good.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to the application of pyrroloquinoline quinone in the preparation of anti-fatigue and alleviating oxidative stress damage products. Background technique [0002] With the increasingly fierce competition, fatigue has become one of the main factors that endanger human physical and mental health. If fatigue is not eliminated in time, it will gradually accumulate, leading to poor mood and aggravating fatigue. Over time, chronic fatigue syndrome will be formed, which greatly affects the work efficiency of ordinary people, the athletic ability of athletes and the combat effectiveness of the army. In recent years, the phenomenon of "death from overwork" has emerged in an endless stream, and it has become younger and younger, which has aroused widespread concern from the whole society. Therefore, how to scientifically and effectively alleviate the damage caused by oxidative stress, delay the appear...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4745A61P39/06A61P39/00A23L33/10
CPCA23V2002/00A61K31/4745A23L33/10A61P39/00A61P39/06A23V2200/30
Inventor 陈骐刘丽霞柯崇榕吴秀琴黄建忠
Owner FUJIAN NORMAL UNIV
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