90 results about "Pseudomonadales" patented technology

Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from Pseudomonas that have undergone co-factor switching to utilize NADH are described.

The present invention relates to Pseudomonas sp. YS-180 having a capability of producing a docosahexaenoic acid and a method for the production of DHA using such a microorganism. Pseudomonas sp. YS-180 was isolated and identified from the intestine of fishes by investigating the morphological, culturing, and physiological characteristics of a strain excellent in a DHA-producing capability. By TLC and GC, Pseudomonas sp. YS-180 according to the present invention was confirmed that it produced the greatest amount of DHA when it was shaking-cultured in a culture containing glucose 2% (w / v), a yeast extract 2% (w / v), and sea water 20%(v / v). Optimal conditions for producing DHA were a pH of 6.0, and a temperature of 20° C. Under these conditions a DHA production was 96.7 mg / L, and a biomass growth was 7.8 g / L.

Provided are compounds and compositions derived from Pseudomonas sp., particularly, Pseudomonas fluorescens or Pseudomonas protegens and more particularly strain having the identifying characteristics of Pseudomonas ATCC 55799 having antimicrobial properties and particularly, antibacterial properties.

A process is provided for the bioconversion of a carbon substrate to 1,3-propanediol by a single organism utilizing microorganisms, such as, Citrobacter, Enterobacter, Clostridium, Klebsiella, Aerobacter, Lactobacillus, Aspergillus, Saccharomyces, Zygosaccharomyces, Pichia, Kluyveromyces, Candida, Hansenula, Debaryomyces, Mucor, Torulopsis, Methylobacter, Escherichia, Salmonella, Bacillus, Streptomyces and Pseudomonas, containing the genes encoding for an active glycerol or diol dehydratase enzyme by contacting these organisms with a carbon substrate under the appropriate fermentation conditions. Specifically, Citrobacter and, Klebsiella provide the source of exogenous genes for such active dehydratase enzyme.

The invention relates to Pseudomonas sp. LD23, and the strain preservation number is CGMCC No,11679. The invention further relates to application of the Pseudomonas sp. LD23 in petroleum degradation and preparation of sodium alginate-activated carbon immobilized microspheres containing the Pseudomonas sp. LD23. The Pseudomonas sp. LD23 is a strain separated and screened from soil which is serious in petroleum pollution, can keep the high petroleum degradation rate under the low temperature condition and lays a foundation for petroleum-polluted soil treatment in future; meanwhile, the biological carbon immobilization conditions are optimized, the degrading effect of the immobilized petroleum degrading bacterium to raw petroleum is initially inspected, and a scientific basis is provided for remedying petroleum oil through the biological carbon immobilized microspheres.

The invention discloses a method for producing glucaric acid by constructing recombinant saccharomyces cerevisiae and fermenting and belongs to the technical field of bioengineering. The method comprises the following steps: knocking out an OPI gene of saccharomyces cerevisiae BY4741; introducing myo inositol-1-phosphate synthase and inositol monophosphatase from the saccharomyces cerevisiae, inositol from arabidopsis thaliana and uronic acid dehydrogenase from pseudomonas syringae into the saccharomyces cerevisiae through constitutive plasmid pY26-GPD-TEF, and realizing approach construction of the saccharomyces cerevisiae from glucose to the glucaric acid. According to the method disclosed by the invention, by use of a constructed metabolic pathway, non-selectivity, high energy consumption property and high cost performance of a chemical oxidation method are avoided; the low-priced glucose is directly transformed into D-glucaric acid with high added value by fermentation culture of engineering bacteria; the method has the advantages of mild reaction conditions, high transformation rate and fewer byproducts; production cost can be greatly reduced.

The invention discloses a preparation technique of a photosynthetic bacteria reagent, which comprises rhodopseudomonas palustris strain cultivation steps that include activated strain cultivation with an inclined plane, strain cultivation with a shaking table, cultivation with an aerated fermenting jar and the like, and adopts the culture medium comprising a basic culture medium and a growth promoting agent. The basic culture medium comprises following components by mass: 0.9 to 1.1 percent of glucose, 0.04 to 0.06 percent of carbamide, 0.02 to 0.03 percent of dipotassium hydrogen phosphate, 0.02 to 0.03 percent of monopotassium phosphate, 0.02 to 0.03 percent of magnesium sulfate, 0.20 to 0.30 percent of sodium chloride, 0.03 to 0.05 percent of calcium chloride and 98.40 to 98.77 percent of water. The added growth promoting agent is 0.10 to 0.30 percent of fulvic acid by mass referring to the basic culture medium. The photosynthetic bacteria reagent that is obtained by the preparation technique can reach the lysate concentration of 4000 to 6000 million per milliliter, and can be used in multiple fields of aquiculture, wastewater processing, crop production and the like.

The invention discloses a genetic engineering strain for producing phenazine-1-carboxylic acid, which is produced by taking pseudomonas chlororaphis as a culture, as well as application of the genetic engineering strain; the genetic engineering strain is obtained by knocking out a phzH gene in pseudomonas chlororaphis genome. The genetic engineering strain for producing phenazine-1-carboxylic acid is finally prepared by deleting the phzH gene in the genome of the strain by virtue of insertion mutation or marker-less deletion from pseudomonas chlororaphis HT66 CCTCC NO: M2013467 which can naturally secrete phenazine-1-formamide as well as derivatives of the pseudomonas chlororaphis so as to convert a secondary metabolite from the phenazine-1-formamide into the phenazine-1-carboxylic acid. The invention also discloses a preparation method and a detection method of the fungicide.

A method for treating HIV infection is disclosed. The method comprises administering to a patient in need thereof a therapeutically effective amount of a fusion protein comprising: a) a Pseudomonas Exotoxin A (PE) peptide comprising a binding domain and a PE translocation domain, the PE peptide being devoid of cytotoxic domain III; b) optionally gag24, being fused to the PE peptide; c) a fragment of gp120 C1 domain, being fused to the PE peptide or fused to the gag24 if the gag24 is present; d) a fragment of gp 120 C5 domain, being fused to the fragment of gp120 C1 domain; e) a fragment of gp41 amino acid sequence, being fused to the fragment of gp 120 C5 domain, and f) optionally an endoplasmic reticulum retention sequence, being fused to the C-terminus of the fragment of gp41.

The invention provides a composition, a composite bacterium agent and application thereof. The composition or the composite bacterium agent comprises a composition of at least six or seven strains from bacillus subtilis, bacillus megatherium, pseudomonad, sphingobacterium thalpophilum, candida tropicalis, bacillus firmus and lactobacillus plantarum. The application includes sludge treatment.

The invention discloses a compound microbial phosphate-solubilizing bacterial agent and a preparation method thereof. The effective ingredients of the compound microbial phosphate-solubilizing bacterial agent comprise pseudomonasveronii, rahnellaaquatilis and bacillusmegaterium. Three types of high-efficiency phosphate-solubilizing strains are obtained by screening soil samples in a high-yield wheat field in the north of Anhui Province, and the collection numbers of the pseudomonasveronii, the rahnellaaquatilis and the bacillusmegaterium are CGMCC No. 8741, CGMCC No. 8739 and CGMCC No. 8737 respectively. The preparation method comprises the following steps: blending and inoculating the pseudomonasveronii and the rahnellaaquatilis into an inorganic phosphorus liquid culture medium and culturing for 48 hours at the temperature of 28-30 DEG C and the speed of 180 r / min; inoculating the bacillusmegaterium into an organic phosphorus liquid culture medium and culturing for 96 hours at the temperature of 28-30 DEG C and the speed of 180 r / min; and uniformly blending a fermented bacteria agent and additives and packing after drying to obtain a finished product. The compound microbial phosphate-solubilizing bacterial agent is capable of remarkably increasing the yield of wheat, rape and other crops.

The invention belongs to the technical field of pyruvic acid preparation method, and relates to a method for preparing pyruvic acid through a process of converting DL-lactic acid by using lactic acid oxidase (LOD) and catalase (CAT) in Pseudomonas fluorescens. The invention provides a method for preparing pyruvic acid through a process of converting DL-lactic acid by using lactic acid oxidase (LOD) and catalase (CAT) in microbe bacterial strain (ATCC 948)-Pseudomonas fluorescens crude enzymes liquor. According to the invention, the conversion rate of the products can be raised and the products stability can be maintained.

The present invention relates to processes for the production of microbial glycolipids, mannosylerythritol lipids (MEL), from lignocellulosic carbon source. These processes are characterized in that the use of lignocellulosic materials for the production of a microbial glycolipids, MEL, comprising a fermentation preferably using fungi of the genus Pseudozyma or other microorganisms such as genetically modified fungi or bacteria. The processes for production of microbial glycolipids, MEL comprise three steps: pretreatment of lignocellulosic material; enzymatic hydrolysis; and fermentation. The enzymatic hydrolysis and fermentation may take place sequentially or simultaneously with addition of exogenous enzymes or simultaneously with enzymes produced by the microorganism itself. The produced microbial glycolipids have applications as: biosurfactants; antimicrobials; anticancer agents; wound healing factors; stabilizer agents on storage and purification of proteins or vaccines; drugs and gene deliver agents; antifreeze agents.

The invention relates to a plasmid characterized in that the plasmid comprises a DNA fragment containing a gene coding for an enzyme taking PQQ as the prosthetic group as cloned in a broad-host-range vector defected of conjugative transfer function beforehand and that the plasmid is capable of being expressed in bacteria of the genus Pseudomonas.

The present invention generally relates to a new approach for the therapy of allergic responses, based on targeted elimination of cells expressing the FcεRI receptor by a chimeric cytotoxin Fc2′-3-PE40. A sequence encoding amino acids 301-437 of the Fc region of the mouse IgE molecule was genetically fused to PE40—a truncated form of PE lacking the cell binding domain. The chimeric protein, produced in E. coli, specifically and efficiently kills mouse mast cell lines expressing the FcεRI receptor, as well as primary mast cells derived from bone marrow. The present invention provides a chimeric protein for targeted elimination of FcεRI expressing cells especially useful for the therapy of allergic responses. The said chimeric protein is comprised of a cell targeting moiety for FcεRI expressing cells and a cell killing moiety. The preferred killing moiety is the bacterial toxin Pseudomonas exotoxin (PE). This Pseudomonas exotoxin is a product of Pseudomonas aeruginosa. The present invention also relates to a method for the preparation of said protein. This chimeric protein is prepared by genetically fusing the Fc region of the mouse IgE molecule to PE40, a truncated form of PE lacking the cell binding domain. The present invention also provides pharmaceutical compositions, for the treatment of allergic diseases and for the treatment of hyperplasias and malignancies, comprising as an active ingredient the above mentioned chimeric protein and a conventional adjuvant product.

The invention provides a compound microbial bacterium for an aerobic fermentation stage. The compound microbial bacterium for an aerobic fermentation stage is prepared by culturing, fermenting, drying and mixing Bacillus licheniformis, Bacillus subtilis, saccharomycete, 5406 actinomycete, Trichoderma viride, Aspergillus oryzae and pseudomonad respectively, wherein the compound microbial bacterium comprises the following components in parts by weight: 0.7-1.2 parts of Bacillus licheniformis, 0.7-0.8 part of Bacillus subtilis, 1.1-1.2 parts of saccharomycete, 1.5-2.5 parts of 5406 actinomycete, 1-2 parts of Trichoderma viride, 1-2 parts of Aspergillus oryzae and 0.5-1 part of pseudomonad. The compound microbial bacterium for an aerobic fermentation stage has phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing capabilities, and can quickly decompose organic substances, so that materials are thoroughly decomposed, and oxidation-resistant substances are generated through metabolism, thus generating a complex and stable ecological system; and the heating speed is high in the whole fermentation process, the temperature can be up to 50 DEG C or above within 1-2 days, and undesirable odor starts to disappear within 24 hours.

The present invention relates to the field of recombinant protein production in bacterial hosts. It further relates to expression of soluble, active recombinant protein by using secretion signals to direct the protein to the periplasmic space of a bacterial cell. In particular, the present invention relates to a production process for obtaining soluble hG-CSF protein from a bacterial host.

The invention relates to a feed additive, and particularly relates to a microbial fermentation additive and a preparation method as well as application thereof. The preparation method of the microbial fermentation additive comprises the following steps of mixing bacillus licheniformis, bacillus subtilis, bifidobacterium bifidum, enterococcus faecalis, lactobacillus acidophilus, lactobacillus casei, lactobacillus lactis, lactobacillus plantarum, pediococcus acidilactici, pediococcus pentosaceus, candida utilis, saccharomyces cerevisiae and rhodopseudomonas palustris microbial inoculum according to a proportion by a viable count per unit mass, which is (500 to 600): (500 to 600): (10,000 to 75,000): (10,000 to 73,000): (10,000 to 75,000): (10,000 to 73,000): (10,000 to 73,000): (10,000 to 65,000): (10,000 to 60,000): (10,000 to 75,000): (10,000 to 75,000): (50 to 70): 1, and obtaining the microbial fermentation additive through preparation. According to the microbial fermentation additive and the preparation method as well as the application thereof, straw is subjected to fermentation treatment through a synergistic effect among strains; the fermentation level of the straw can be quickly improved; the deficiency that a single strain secretes enzymes and synthetics is made up; the fermentation quality is improved; meanwhile, by generating many kinds of organic acid and mycoprotein, the palatability of the straw can be improved; the feed intakes of livestock and poultry are improved; the production properties of the livestock and the poultry are obviously improved.

The present invention relates to a new use of a vaccine comprising a fusion protein that comprises the Pseudomonas aeruginosa outer membrane protein I (OprI or OMPI) which is fused with its amino terminal end to the carboxy-terminal end of a carboxy-terminal portion of the Pseudomonas aeruginosa outer membrane protein F (OprF or OMPF), as well as to a new use of a monoclonal or polyclonal antibody against this fusion protein or a pharmaceutical composition thereof.

The invention discloses a pseudomonas strain and a method for preparing a composite surfactant by using the same and relates to a microbial strain and a method for preparing an active agent. The strain is named as pseudomonas which is preserved in China Center for Type Culture Collection (CCTCCNOM) on November 13, 2013 and has a preservation number of CCTCCNO: M2013570; the invention discloses a pseudomonas CCTCCNO: M2013570 and an application thereof. The strain disclosed by the invention can produce the composite surfactant, namely, glycolipid and amino-acid-containing lipoids, during a fermentation process by virtue of a plurality of carbon sources; the strain, as well as the fermentation broth and surfactant extracts thereof have good application prospects in wastewater treatment, treatment of soil heavy metal pollution, preservation and bacteria inhibition of foods such as fruits and vegetables, washing and daily chemical products and the like. The synergistic effects of different biological surfactants can be given and the strain can play a role under various conditions.

The invention discloses a microorganism antioxidant of edible vegetable fat as well as a preparation method thereof. The microorganism antioxidant of edible vegetable fat is prepared by mixing polyglycerol monooleate, alcohol and microorganism antioxidant fermentation liquor in proportion, wherein production strains of the microorganism antioxidant fermentation liquor include at least one of rhodopuesdomonas pulstris, saccharomyces cerevisiae and actic acid bacteria. The preparation method comprises the preparation steps of: preparing rhodopuesdomonas pulstris, saccharomyces cerevisiae, lactobacillus plantarum, streptococcus thermophilus as well as bifidobacterium; weighing the cultured strains according to a proportion to prepare strain stock liquor; mixing the strain stock liquor and culture medium, centrifuging after fermenting, sterilizing and disinfecting to obtain the needed microorganism antioxidant fermentation liquor; and mixing the polyglycerol monooleate, alcohol and microorganism antioxidant fermentation liquor in proportion, dispersing and stewing to obtain the needed microorganism antioxidant. The invention provides a natural antioxidant which is capable of restraining edible vegetable fat oxidization and prolonging a quality guarantee period as well as a preparation method thereof. The microorganism antioxidant of edible vegetable fat disclosed by the invention is enabled to satisfy requirements of high efficiency, safety and low production cost, and mouthfeel-strengthening of the fat.

The invention discloses a pseudomonic acid A producing strain and application thereof. The pseudomonic acid A producing strain is pseudomonas fluorescens, and the preservation number is CGMCC No.7147. The pseudomonas fluorescens has very high pseudomonic acid A fermentability, and the capability of the pseudomonas fluorescens for producing the pseudomonic acid A is many times that of the conventional producing strain, and more than 5 times that of the starting strain; the fermentation unit of the pseudomonas fluorescens can reach more than 2500 mg / L in a 5000 L fermentation tank; moreover, the fermentation product compositions of the pseudomonas CGMCC No.7147 is simple, and has no analogues of the pseudomonic acid A, so that the cost of separating and purifying the pseudomonic acid A from fermentation liquor is reduced greatly.

The invention discloses a method for producing optically pure L-alanine by one pseudomonad and two enzymes, comprising the following steps of: seed culture in a shake flask and fermentation; utilizing pseudomonas to generate two enzymes: an L-aspartase and an L-aspartic acid-Beta-decarboxylase; taking a fumaric acid ammonium salt as a substrate to directly generate the L-alanine; and obtaining the optically pure L-alanine by changing the system reaction condition and adding the L-alanine racemase inhibitor. The method reduces the whole production link and equipment investment for the L-alanine, lightens the environmental pollution, has high yield of L-alanine, has the optical purity of more than or equal to 99.99% and meets the requirements of factories that have special requirements on the L-alanine with high optical purity.

The invention relates to a composite bacterium capable of quickly degrading 17Beta-estradiol. The composite bacterium can be used to quickly degrade steroid estrogen-17Beta-estradiol in livestock breeding waste, reducing environmental pollution and resourcefully using livestock excrement. The composite bacterium that can quickly degrade 17Beta-estradiol is composed of Acinetobacter calcoaceticus LM1 collected under CGMCC No. 10814 and Pseudomonas LY1 collected under CGMCC No. 10815. Mass experiments prove that Acinetobacter calcoaceticus LM1 and Pseudomonas LY1 are mixed according to a cell ratio of 1:1, giving optimal degrading efficiency for treating livestock breeding waste and 17Beta-estradiol in soil.

The invention belongs to the technical field of agricultural microbiology, and particularly relates to the separation screening and fermentation cultivation of phosphorus-dissolving pseudomonas putida L13. A phosphorus-dissolving pseudomonas putida L13 strain with the capacity of dissolving phosphorus is obtained through separation, and the preservation No. is CCTCC No.2010342. The fermentation process comprises the following steps of: (1) taking L13 bacterial strain slope seeds for streak cultivation on an LB (lysogeny broth) culture medium flat plate to obtain first-level seeds; (2) inoculating a first-level seed single bacterial colony into an LB culture medium shake flask to make OD (optical density) 600 be 2.0 to obtain second-level seeds; (3) inoculating the second-level seeds into a shake flask for fermentation cultivation to obtain the pseudomonas putida; or inoculating the second-level seeds into a fermentation tank so as to obtain the pseudomonas putida through optimized cultivation. The pseudomonas putida strain disclosed by the invention grows rapidly, the yield of the pseudomonas putida is high, and the viable count can reach more than 10 billions. The phosphorus-dissolving amount of the phosphorus-dissolving pseudomonas putida in a PKO (Pikovskaya') culture medium in which 1-3g / L of calcium phytate is the unique phosphorus source can reach 120-220mg / L, and the phosphorus-dissolving amount of the phosphorus-dissolving pseudomonas putida in a PKO culture medium in which 4-6g / L of tricalcium phosphate is the unique phosphorus source can reach 300-500mg / L.

The invention relates to a special white shrimp breeding composite bacterium flora which comprises 9% of bdellovibrio BDS01, 4% of bdellovibrio BDS02, 5% of nitrobacterium, 3% of streptococcus thermophilus, 12% of yeast, 1.5% of lactobacillus bulgaricus, 1.5% of leuconostoc, 5% of aerobic denitrification bacteria, 0.5% of pediococcus, 5% of bacillus subtilis BDS-2, 2.5% of rhodobacter D8-35, 1% ofactinomycetes S317, 1% of a bacillus B135, 5% of yeast E7, 5% of acetobacterium balch, 5% of methane producing bacteria, 4% of lactic acid bacteria L2, 3% of aerobic spore-bearing bacilli, 2% of flavobacterium, 5% of bacillus coagulans, 3% of anaerobic lactobacillus, 1% of rhodop seudanonas palustris, 0.5% of pseudoalteromonas, 0.5% of rhodobacter azotoformans, 0.5% of firmicutes, 1% of lipoidase, 0.5% of protease, 1% of cyanobacteria, 3% of aerobic denitrification bacteria, 0.5% of actinomycetes, 0.2% of bacillus natto, 0.5% of viable bacteria of clostridium butyricum, 3% of photosynthetic bacteria and 0.8% of photosynthetic bacteria. Harmful indexes such as CODs (Chemical Oxygen Demands), ammonia nitrogen,nitrite and the like can be effectively controlled.

A Pseudomonas sp. strain TKU015 is deposited under DSMZ GmbH (Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH) Number DSM 21747). The Pseudomonas sp. strain TKU015 can be used to produce chitinase, chitosanase and nattokinase. A method of producing chitinase, chitosanase and nattokinases can use the Pseudomonas sp. strain TKU015.

The invention discloses a genetic engineering strain for producing DHHA (2,3-dihydro-3-hydroxyanthranilic acid) and the application thereof. On the basis of pseudomonas chlororaphis HT66 CCTCC NO: M2013467 secreting phenazinyl-1-carboxamide, a gene phzF in genomes of the strain and derivatives thereof is knocked out in the phenazine synthesizing route, so that an intermediate metabolite DHHA is accumulated and a phenazine compound is not synthesized any more. The genetic engineering strain can be applied to the preparation of the DHHA. The DHHA can be used as an important organic synthetic intermediate and a chiral catalyst, and has an important application value. The genetic engineering strain is safe, reliable, good in stability and short in fermentation period, and is conducive to biosynthesis and industrial application of the DHHA.