51 results about "Alanine racemase" patented technology

The invention discloses an XZ-A26 bacterial strain for producing L-alanine with high yield, which has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.4036 and has the capacity of generating high-concentration L-alanine through fermentation. The XZ-A26 bacterial strain is constructed by the steps of: integrating an L-alanine dehydrogenase gene on thermophilic fatty bacillus chromosome on lactic dehydrogenase position on an escherichia coli ATCC8739 chromosome, then sequentially knocking out a pyruvate formate lyase gene, an alcohol dehydrogenase gene, an acetokinase gene, a fumaric acid reductase gene and an alanine racemase gene of the escherichia coli chromosome, and then carrying out continuous cell culture in a fermenting tank for obtaining agenetic engineering strain. The invention also relates to a construction method of the XZ-A26 bacterial strain and an application of the XZ-A26 bacterial strain in preparation of the L-alanine. According to the invention, the escherichia coli with the preservation number of CGMCC No.4036 for generating the high-concentration L-alanine through fermentation can be constructed by using a metabolic engineering method, and the yield of the L-alanine generated by using the XZ-A26 bacterial strain reaches up to 115g / L. The XZ-A26 bacterial strain is suitable for industrially producing the L-alanine.

Disclosed is an alanine racemase chiral binaphthol derivative having the ability to recognize amino alcohols selectively on the basis of chirality and transform amino acids from an L-form into a D-form. Methods for the optical resolution of amino acid or amino alcohol and for the optical transformation of D- and L-forms of amino acids using the binaphthol derivative are also provided.

The invention discloses a method for constructing a recombinant Bacillus subtilis expressing D-psicose 3-epimerase based on a D-alanine-deficient selection marker, belonging to the technical field of enzyme gene engineering. Taking Bacillus subtilis 1A751 as the starting strain, knocking out the D-alanine racemase gene (dal) on its chromosome to obtain D-alanine-deficient 1A751 (dal-); the DPE of Clostridium ATCC 35704 The enzyme gene was used as a parent, and its upstream fusion P43 promoter was constructed into P43‑DPE, which was constructed into plasmid pUB110 (NCBI‑Gene ID: 9507338) to obtain pUB‑P43‑DPE, and the antibiotic resistance on pUB‑P43‑DPE The sex genes Kan and Blm were replaced by dal to construct pUB-P43-DPE-dal; then transformed into 1A751(dal-) to obtain recombinant Bacillus subtilis 1A751-pUB-P43-DPE-dal, which was named as Bacillus subtilis ( Bacillus subtilis) SK38.001, deposit number CCTCC NO:M 2015257. The total enzyme activity of its fermented liquid reaches 16U / mL, which has important industrial application value.

The invention discloses an XZ-A26 bacterial strain for producing L-alanine with high yield, wherein the preservation number is CGMCC No.4036. The XZ-A26 bacterial strain has the ability of fermenting to produce high-concentration L-alanine and is genetic engineering bacteria prepared by integrating the L-alanine dehydrogenase gene on Escherichia Coli chromosome of a lipase gene from Geobacillus Stearothermophilus at the lactic dehydrogenase of Escherichia coli ATCC 8739 chromosome, then sequentially removing a pyruvate formate-lyase gene, an ethanol dehydrogenase gene, an acetokinase gene, a fumarate reductase gene and an alanine racemase gene of the escherichia coli chromosome and then continuously subculturing in a fermentation tank. The invention also relates to a construction method of the bacterial strain and the application of the bacterial strain in preparing the L-alanine. According to the metabolic engineering method, the escherichia coli CGMCC No.4036 capable of fermenting to produce the high-concentration L-alanineis constructed, and the yield of the L-alanine produced by fermenting the bacterial strain is as high as 115g / L. The invention is suitable for the industrial production of L-alanine.

The invention provides alanine racemase encoding polynucleotides, which are capable of conferring increased nematode resistance in a plant. Specifically, the invention relates to methods of producing transgenic plants with increased nematode resistance, expression vectors comprising polynucleotides encoding alanine racemase, and transgenic plants and seeds generated thereof.

The present disclosure provides recombinant Staphylococcus bacterium (e.g. S. epidermidis) that are dependent on D-alanine for growth. In one aspect, the disclosure features a recombinant Staphylococcus bacterium comprising two inactivated alanine racemase genes (Δalr1Δalr2); and an inactivated D-alanine aminotransferase (dat) gene. In another aspect, the disclosure features a method of making the recombinant Staphylococcus bacterium.

The invention provides a strain for producing high-yield DL-alanine and an application thereof, and particularly provides an engineering strain for producing D-alanine racemase. The strain is esherichia coli and carries an expression vector for expressing Bacillus subtilis D-alanine racemase. The expression strain can be effectively used for producing the DL-alanine at a low cost, and has a higher application value.

The invention discloses genetic engineering bacteria containing L-alanine racemase genes and application of genetic engineering bacteria; the bacterial strain comprises a nucleotide sequence expressed by SEQ ID NO: 1. The genetic engineering bacteria disclosed by the invention can produce DL-alanine through racemizing L-alanine and is characterized by little dosage, short reaction time and multi-batch reutilization; by adoption of the genetic engineering bacteria of The invention, conversation rate in a racemizing procedure reaches to more than 99.0%; product purity reaches to more than 99.5%; and the genetic engineering bacteria disclosed by The invention has good industrialization prospect.