The invention discloses a beef flavor potato chip. The beef flavor potato chip is prepared through the steps of material selection, raw material pretreatment, slicing, color protection, dewatering, oil frying, seasoning, package and the like. Spices of the beef flavor potato chip are prepared from the following ingredients in percentage by weight: 15 to 20 percent of fine beef powder, 5 to 10 percent of minced beef, 5 to 10 percent of fine pork powder, 5 to 9 percent of salt, 5 to 10 percent of white sugar, 5 to 8 percent of monosodium glutamate, 5 to 10 percent of chilli powder, 5 to 10 percent of black pepper powder, 1 to 3 percent of yeast extracts, 0.5 to 0.8 percent of chrysanthemum powder, 5 to 10 percent of xylitol, 0.2 to 0.4 percent of orange peel powder, 1 to 2 percent of turmeric powder, 2 to 3 percent of bighead atractylodes rhizome powder, 2 to 4 percent of hawthorn fruit powder, 1 to 2 percent of salvia miltiorrhiza powder, 0.5 to 1 percent of cortex moutan powder and 1 to 2 percent of agaricus blazei murrill powder. The beef flavor potato chip manufactured according to the invention has the advantages that the flavor is novel, the beef flavor potato chip is healthy and nutritive, the eating is convenient, the process is simple, the technology can be easily mastered, the investment is low, the effect taking speed is high, the general popularization is convenient,and the standardized, normalized and factory production can be easily realized.